| Abstract | Photosynthetic performance of algal-bacterial biofilms from an Italian wastewater treatment plant was studied in a flow-lane photobioreactor at different irradiances, temperatures, and flow regime to evaluate the effects of these environmental parameters on biofilms' functioning, in view of application of these communities in wastewater biological treatment. Pulse amplitude modulated fluorescence was used to estimate the effective quantum yield of PSII (?F/F') of the light-acclimated biofilms and to perform rapid light curves (RLCs) for the determination of the photosynthetic parameters (rel.ETR, ?, I ). Chl a, ash free dry weight (AFDW), and dry weight (DW) were measured to assess phototrophic and whole biofilm biomass development over time. From the analysis of photosynthetic parameter variation with light intensity, temperature and flow rate, it was possible to identify the set of experimental values favoring biofilm photosynthetic activity. Biomass increased over time, especially at the highest irradiances, where substrata were fastly colonized and mature biofilms developed at all temperatures and flow conditions tested. |
| Text | 437873 2019 10.3390/microorganisms7080252 Scopus 2 s2.0 85074360865 ISI Web of Science WOS WOS 000443144800012 phototrophic biofilm; wastewater; photosynthesis; photobioreactor; Pulse Amplitude Modulated fluorescence; biofilm biomass Wastewater biofilm photosynthesis in photobioreactors Guzzon A.; Di Pippo F.; Congestri R. LBA Laboratory of Biology of Algae, Department of Biology, University of Rome Tor Vergata , Rome, 00133, LBA Laboratory of Biology of Algae, Department of Biology, University of Rome Tor Vergata , 00133, Rome, Italy, , Italy; CNR IRSA, National Research Council, Water Research Institute, Area della Ricerca di Roma 1, Montelibretti, RM, 00010, CNR IRSA, National Research Council, Water Research Institute, Area della Ricerca di Roma 1, 00010, Montelibretti , RM , Italy, , Italy Photosynthetic performance of algal bacterial biofilms from an Italian wastewater treatment plant was studied in a flow lane photobioreactor at different irradiances, temperatures, and flow regime to evaluate the effects of these environmental parameters on biofilms functioning, in view of application of these communities in wastewater biological treatment. Pulse amplitude modulated fluorescence was used to estimate the effective quantum yield of PSII F/F of the light acclimated biofilms and to perform rapid light curves RLCs for the determination of the photosynthetic parameters rel.ETR, , I . Chl a, ash free dry weight AFDW , and dry weight DW were measured to assess phototrophic and whole biofilm biomass development over time. From the analysis of photosynthetic parameter variation with light intensity, temperature and flow rate, it was possible to identify the set of experimental values favoring biofilm photosynthetic activity. Biomass increased over time, especially at the highest irradiances, where substrata were fastly colonized and mature biofilms developed at all temperatures and flow conditions tested. 7 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85074360865 origin=inward Articolo in rivista Molecular Diversity Preservation International 2076 2607 Microorganisms Microorganisms francesca.dipippo DI PIPPO FRANCESCA |
