| Title | Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process |
| Abstract | Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethoxazole) in animal husbandry practices is showing increasing negative impacts resulting from the release of still metabolically active molecules into agroecosystems. In the present study, cattle manure collected from an AD plant-feeding tank was used as feedstock for AD experiments in which some batches were spiked with 5 mg L-1 of sulfamethoxazole (SMX). Adding the antibiotic affected the microbial community dynamic; in particular, the efficiency of the acidogenic and acetogenic phases of the process corresponded to higher CH4 and H2 production than in the control. SMX was also degraded, and at the end of the experiment (69 days), just 20% of its initial concentration was found. The relative abundance (ARG/16S) of resistance genes sul1, sul2, and the proxy intI1 initially found in the ingestate decreased during the AD in both the spiked and control batches, suggesting that this process lowers the likelihood of antibiotic resistance genes spreading. |
| Source | Frontiers in microbiology 11, pp. 1–12 |
| Keywords | cattle manuresulfamethoxazolebiogasanaerobic digestionmicrobial communityantibiotic degradationARGs |
| Journal | Frontiers in microbiology |
| Editor | Frontiers Research Foundation,, Lausanne, Svizzera |
| Year | 2020 |
| Type | Articolo in rivista |
| DOI | 10.3389/fmicb.2020.537783 |
| Authors | Mazzurco Miritana V, Massini G, Visca A, Grenni P, Patrolecco L, Spataro F, Rauseo J, Garbini GL, Signorini A, Rosa S and Barra Caracciolo A |
| Text | 431574 2020 10.3389/fmicb.2020.537783 cattle manure sulfamethoxazole biogas anaerobic digestion microbial community antibiotic degradation ARGs Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process Mazzurco Miritana V, Massini G, Visca A, Grenni P, Patrolecco L, Spataro F, Rauseo J, Garbini GL, Signorini A, Rosa S and Barra Caracciolo A Water Research Institute, National Research Council, Montelibretti, Italy Department of Energy Technologies, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, Italy Institute of Polar Sciences, National Research Council, Montelibretti, Italy Anaerobic digestion AD treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics e.g., sulfamethoxazole in animal husbandry practices is showing increasing negative impacts resulting from the release of still metabolically active molecules into agroecosystems. In the present study, cattle manure collected from an AD plant feeding tank was used as feedstock for AD experiments in which some batches were spiked with 5 mg L 1 of sulfamethoxazole SMX . Adding the antibiotic affected the microbial community dynamic; in particular, the efficiency of the acidogenic and acetogenic phases of the process corresponded to higher CH4 and H2 production than in the control. SMX was also degraded, and at the end of the experiment 69 days , just 20% of its initial concentration was found. The relative abundance ARG/16S of resistance genes sul1, sul2, and the proxy intI1 initially found in the ingestate decreased during the AD in both the spiked and control batches, suggesting that this process lowers the likelihood of antibiotic resistance genes spreading. 11 Published version Effects of sulfamethoxazole on the microbial community dynamics during the anaerobic digestion process PDF ARTICOLO FrontiersMicrobialEcologySulfaADprocess_11 537783.pdf Articolo in rivista Frontiers Research Foundation, 1664 302X Frontiers in microbiology Frontiers in microbiology Front. microbiol. Frontiers in microbiology VISCA ANDREA GARBINI GIAN LUIGI MAZZURCO MIRITANA VALENTINA luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA francesca.spataro SPATARO FRANCESCA jasmin.rauseo RAUSEO JASMIN DTA.AD002.414.001 Valutazione della Presenza di Antibiotici nei Reflui Zootecnici e nel Digestato di Impianti a Biogas Studio di Strategie per la loro Rimozione |
