| Title | A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants |
| Abstract | The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum ?-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 10 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status. |
| Source | Environment international 144 |
| Keywords | Antibiotic resistanceColiformsESBLsWastewater treatmentWater reuse |
| Journal | Environment international |
| Editor | Pergamon., New York, Stati Uniti d'America |
| Year | 2020 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.envint.2020.106035 |
| Authors | Marano, Roberto B.M.; Fernandes, Telma; Manaia, Célia M.; Nunes, Olga; Morrison, Donald; Berendonk, Thomas U.; Kreuzinger, Norbert; Telson, Tanel; Corno, Gianluca; Fatta-Kassinos, Despo; Merlin, Christophe; Topp, Edward; Jurkevitch, Edouard; Henn, Leonie; Scott, Andrew; Heß, Stefanie; Slipko, Katarzyna; Laht, Mailis; Kisand, Veljo; Di Cesare, Andrea; Karaolia, Popi; Michael, Stella G.; Petre, Alice L.; Rosal, Roberto; Pruden, Amy; Riquelme, Virginia; Agüera, Ana; Esteban, Belen; Luczkiewicz, Aneta; Kalinowska, Agnieszka; Leonard, Anne; Gaze, William H.; Adegoke, Anthony A.; Stenstrom, Thor A.; Pollice, Alfieri; Salerno, Carlo; Schwermer, Carsten U.; Krzeminski, Pawel; Guilloteau, Hélène; Donner, Erica; Drigo, Barbara; Libralato, Giovanni; Guida, Marco; Bürgmann, Helmut; Beck, Karin; Garelick, Hemda; Tacão, Marta; Henriques, Isabel; Martínez-Alcalá, Isabel; Guillén-Navarro, Jose M.; Popowska, Magdalena; Piotrowska, Marta; Quintela-Baluja, Marcos; Bunce, Joshua T.; Polo-López, Maria I.; Nahim-Granados, Samira; Pons, Marie Noëlle; Milakovic, Milena; Udikovic-Kolic, Nikolina; Ory, Jérôme; Ousmane, Traore; Caballero, Pilar; Oliver, Antoni; Rodriguez-Mozaz, Sara; Balcazar, Jose L.; Jäger, Thomas; Schwartz, Thomas; Yang, Ying; Zou, Shichun; Lee, Yunho; Yoon, Younggun; Herzog, Bastian; Mayrhofer, Heidrun; Prakash, Om; Nimonkar, Yogesh; Heath, Ester; Baraniak, Anna; Abreu-Silva, Joana; Choudhury, Manika; Munoz, Leonardo P.; Krizanovic, Stela; Brunetti, Gianluca; Maile-Moskowitz, Ayella; Brown, Connor; Cytryn, Eddie |
| Text | 428865 2020 10.1016/j.envint.2020.106035 Scopus 2 s2.0 85089582870 Antibiotic resistance Coliforms ESBLs Wastewater treatment Water reuse A global multinational survey of cefotaxime resistant coliforms in urban wastewater treatment plants Marano, Roberto B.M.; Fernandes, Telma; Manaia, Celia M.; Nunes, Olga; Morrison, Donald; Berendonk, Thomas U.; Kreuzinger, Norbert; Telson, Tanel; Corno, Gianluca; Fatta Kassinos, Despo; Merlin, Christophe; Topp, Edward; Jurkevitch, Edouard; Henn, Leonie; Scott, Andrew; Heß, Stefanie; Slipko, Katarzyna; Laht, Mailis; Kisand, Veljo; Di Cesare, Andrea; Karaolia, Popi; Michael, Stella G.; Petre, Alice L.; Rosal, Roberto; Pruden, Amy; Riquelme, Virginia; Aguera, Ana; Esteban, Belen; Luczkiewicz, Aneta; Kalinowska, Agnieszka; Leonard, Anne; Gaze, William H.; Adegoke, Anthony A.; Stenstrom, Thor A.; Pollice, Alfieri; Salerno, Carlo; Schwermer, Carsten U.; Krzeminski, Pawel; Guilloteau, Helene; Donner, Erica; Drigo, Barbara; Libralato, Giovanni; Guida, Marco; Burgmann, Helmut; Beck, Karin; Garelick, Hemda; Tacão, Marta; Henriques, Isabel; Martinez Alcala, Isabel; Guillen Navarro, Jose M.; Popowska, Magdalena; Piotrowska, Marta; Quintela Baluja, Marcos; Bunce, Joshua T.; Polo Lopez, Maria I.; Nahim Granados, Samira; Pons, Marie Noelle; Milakovic, Milena; Udikovic Kolic, Nikolina; Ory, Jerome; Ousmane, Traore; Caballero, Pilar; Oliver, Antoni; Rodriguez Mozaz, Sara; Balcazar, Jose L.; Jager, Thomas; Schwartz, Thomas; Yang, Ying; Zou, Shichun; Lee, Yunho; Yoon, Younggun; Herzog, Bastian; Mayrhofer, Heidrun; Prakash, Om; Nimonkar, Yogesh; Heath, Ester; Baraniak, Anna; Abreu Silva, Joana; Choudhury, Manika; Munoz, Leonardo P.; Krizanovic, Stela; Brunetti, Gianluca; Maile Moskowitz, Ayella; Brown, Connor; Cytryn, Eddie Laboratoire Microorganismes Genome et Environnement; Universite Clermont Auvergne; University of Exeter Medical School; Catalan Institute for Water Research; CIEMAT Plataforma Solar de Almeria; Istituto di Ricerca Sulle Acque, Bari; Universidade Catolica Portuguesa; Universite de Lorraine; Eesti Keskkonnauuringute Keskus; University of Cyprus; University of Tartu; Gwangju Institute of Science and Technology; University of South Australia; Universidad de Alcala; Karlsruhe Institute of Technology, Campus North; Virginia Polytechnic Institute and State University; Gda sk University of Technology; Norsk institutt for vannforskning; Universidade de Aveiro; Jozef Stefan Institute; University of Uyo; Sun Yat Sen University; Universidade de Coimbra; Agriculture et Agroalimentaire Canada; Technical University of Munich; Technische Universitat Dresden; Edinburgh Napier University; Technische Universitat Wien; Universita degli Studi di Napoli Federico II; Universidad de Almeria; National Medicines Institute, Warsaw; National Centre for Cell Science India; Middlesex University; University of Warsaw; Western University; Hebrew University of Jerusalem; Centre Hospitalier Universitaire de Clermont Ferrand; Universidade do Porto; Agricultural Research Organization of Israel; Newcastle University, United Kingdom; Durban University of Technology; Eawag Swiss Federal Institute of Aquatic Science and Technology; Institute Ruder Boskovic; UMR 7564 University of Lorraine CNRS LCPME ; CNR IRSA Molecular Ecology Group; Laboratori EMATSA The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants WWTPs by targeting cefotaxime resistant CTX R coliforms as indicators. The rationale for this approach was i coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii CTX R coliforms are clinically relevant, associated with extended spectrum lactamases ESBLs , and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly <0.1% to 38.3% , being positively correlated p < 0.001 with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX R coliforms, loads over 10 colony forming units per mL were occasionally observed in final effluents. We demonstrate that CTX R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status. 144 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85089582870 origin=inward Articolo in rivista Pergamon. 0160 4120 Environment international Environment international Environ. int. Environment international. alfieri.pollice POLLICE ALFIERI gianluca.corno CORNO GIANLUCA andrea.dicesare DI CESARE ANDREA carlo.salerno SALERNO CARLO |
