Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Isolation and Characterization in a Soil Conditioned With Foaming Agents of a Bacterial Consortium Able to Degrade Sodium Lauryl Ether Sulfate |
| Abstract | The anionic surfactant Sodium Lauryl Ether Sulfate (SLES) is the principal component of several commercial foaming products for soil conditioning in the tunneling industry. Huge amounts of spoil material are produced during the excavation process and the presence of SLES can affect its re-use as a by-product. Anionic surfactants can be a risk for ecosystems if occurring in the environment at toxic concentrations. SLES biodegradability is a key issue if the excavated soil is to be reused. The aim of this study was to identify bacteria able to degrade SLES, so that it could potentially be used in bioaugmentation techniques. Enrichment cultures were performed using bacterial populations from spoil material collected in a tunnel construction site as the inoculum. A bacterial consortium able to grow in a few hours with SLES concentrations from 125 mg/L to 2 g/L was selected and then identified by Next Generation Sequencing analysis. Most of bacteria identified belonged to Gamma-Proteobacteria (99%) and Pseudomonas (ca 90%) was the predominant genus. The bacterial consortium was able to degrade 94% of an initial SLES concentration of 250 mg/L in 9 h. A predictive functional analysis using the PICRUSt2 software showed the presence of esterase enzymes, responsible for SLES degradation. The bacterial consortium selected could be useful for its possible seeding (bioaugmentation) on spoil material from tunneling excavation. |
| Source | Frontiers in microbiology |
| Keywords | anionic surfactantfoaming agentsbiodegradationtunnelingspoil materialbioaugmentation |
| Journal | Frontiers in microbiology |
| Editor | Frontiers Research Foundation,, Lausanne, Svizzera |
| Year | 2020 |
| Type | Articolo in rivista |
| DOI | 10.3389/fmicb.2020.01542 |
| Authors | Ludovica Rolando, Paola Grenni, Jasmin Rauseo, Tanita Pescatore, Luisa Patrolecco, Gian Luigi Garbini, Andrea Visca and Anna Barra Caracciolo |
| Text | 425710 2020 10.3389/fmicb.2020.01542 anionic surfactant foaming agents biodegradation tunneling spoil material bioaugmentation Isolation and Characterization in a Soil Conditioned With Foaming Agents of a Bacterial Consortium Able to Degrade Sodium Lauryl Ether Sulfate Ludovica Rolando, Paola Grenni, Jasmin Rauseo, Tanita Pescatore, Luisa Patrolecco, Gian Luigi Garbini, Andrea Visca and Anna Barra Caracciolo 1 Water Research Institute National Research Council IRSA CNR , Monterotondo, Italy, 2 Department of Ecological and Biological Sciences, Tuscia University, Viterbo, Italy, 3 Institute of Polar Sciences National Research Council ISP CNR , Monterotondo, Italy The anionic surfactant Sodium Lauryl Ether Sulfate SLES is the principal component of several commercial foaming products for soil conditioning in the tunneling industry. Huge amounts of spoil material are produced during the excavation process and the presence of SLES can affect its re use as a by product. Anionic surfactants can be a risk for ecosystems if occurring in the environment at toxic concentrations. SLES biodegradability is a key issue if the excavated soil is to be reused. The aim of this study was to identify bacteria able to degrade SLES, so that it could potentially be used in bioaugmentation techniques. Enrichment cultures were performed using bacterial populations from spoil material collected in a tunnel construction site as the inoculum. A bacterial consortium able to grow in a few hours with SLES concentrations from 125 mg/L to 2 g/L was selected and then identified by Next Generation Sequencing analysis. Most of bacteria identified belonged to Gamma Proteobacteria 99% and Pseudomonas ca 90% was the predominant genus. The bacterial consortium was able to degrade 94% of an initial SLES concentration of 250 mg/L in 9 h. A predictive functional analysis using the PICRUSt2 software showed the presence of esterase enzymes, responsible for SLES degradation. The bacterial consortium selected could be useful for its possible seeding bioaugmentation on spoil material from tunneling excavation. Published version 15/06/2020 Isolation and Characterization in a Soil Conditioned With Foaming Agents of a Bacterial Consortium Able to Degrade Sodium Lauryl Ether Sulfate pdf con doi FrontiersinMicrobialEcologyRolandoetal2020.pdf Articolo in rivista Frontiers Research Foundation, 1664 302X Frontiers in microbiology Frontiers in microbiology Front. microbiol. Frontiers in microbiology ROLANDO LUDOVICA tanitapescatore PESCATORE TANITA VISCA ANDREA GARBINI GIAN LUIGI luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA jasmin.rauseo RAUSEO JASMIN ICT.P07.016.003 Sistemi e strumenti di gestione dei Registri nell Internet di nuova generazione WKP1 |