| Title | Short-term and long-term effects on carbon storage of pulse feeding on acclimated or unacclimated or unacclimated activated sludge |
| Abstract | This study was aimed to investigate the effect of different feeding patterns on the physiological state of the activated sludge and related microbial composition in an SBR (SRT of 2 days, acetate as the sole carbon source, aerobic conditions). The activated sludge was acclimated to two subsequent feeding patterns, namely to continuous feeding throughout the reaction phase and then to pulse feeding. FISH and microscopy staining procedure (Nile blue) were used to investigate the microbial composition, in combination with quantitative determination of storage. At steady state, storage was significant even under continuous feeding whereas pulse feeding brought a strong increase of both rate and yield of storage. Short-term and long-term effects were clearly distinguishable: the immediate adaptation
of biomass coming from continuous feeding to a single spike accounted for a significant portion of the overall increase of both rate and yield of polymer storage that was obtained after long acclimation to pulse feeding. On the contrary, after either type of feeding, both cultures were mainly constituted from the members of Thauera/Azoarcus group. Thus, the same dominant group preferably consumed the acetate via storage or growth depending on acclimation conditions. Our study clearly showed that a progressive increase of storage capacity is not necessarily due to a shift of microbial composition. |
| Source | Water research (Oxf.) 45, pp. 3119–3128 |
| Keywords | Activated sludge; PHA storage; Microbial composition; FISH; Feeding pattern |
| Journal | Water research (Oxf.) |
| Editor | Pergamon Press., New York, Regno Unito |
| Year | 2011 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.watres.2011.03.026 |
| Authors | Ciggin A., Orhon D., Rossetti S., Majone M. |
| Text | 42435 2011 10.1016/j.watres.2011.03.026 Activated sludge; PHA storage; Microbial composition; FISH; Feeding pattern Short term and long term effects on carbon storage of pulse feeding on acclimated or unacclimated or unacclimated activated sludge Ciggin A., Orhon D., Rossetti S., Majone M. Faculty of Civil Engineering, Environmental Engineering Department, Istanbul Technical University, 34469 Maslak, Istanbul, Turkey Turkish Academy of Sciences, Piyade sokak No. 27, 06550 C¸, Ankara, Turkey Istituto di Ricerca Sulle Acque C.N.R., Via Salaria Km 29,300, 00016 Monterotondo, Italy Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy This study was aimed to investigate the effect of different feeding patterns on the physiological state of the activated sludge and related microbial composition in an SBR SRT of 2 days, acetate as the sole carbon source, aerobic conditions . The activated sludge was acclimated to two subsequent feeding patterns, namely to continuous feeding throughout the reaction phase and then to pulse feeding. FISH and microscopy staining procedure Nile blue were used to investigate the microbial composition, in combination with quantitative determination of storage. At steady state, storage was significant even under continuous feeding whereas pulse feeding brought a strong increase of both rate and yield of storage. Short term and long term effects were clearly distinguishable the immediate adaptation of biomass coming from continuous feeding to a single spike accounted for a significant portion of the overall increase of both rate and yield of polymer storage that was obtained after long acclimation to pulse feeding. On the contrary, after either type of feeding, both cultures were mainly constituted from the members of Thauera/Azoarcus group. Thus, the same dominant group preferably consumed the acetate via storage or growth depending on acclimation conditions. Our study clearly showed that a progressive increase of storage capacity is not necessarily due to a shift of microbial composition. 45 Published version Short term and long term effects on carbon storage of pulse feeding on acclimated or unacclimated activated sludge pdf della pubblicazione water_Res_2011.pdf Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. simona.rossetti ROSSETTI SIMONA TA.P07.017.001 Caratterizzazione di biomasse per l ottimizzazione dei processi biologici |
