Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Metaproteome analysis of sewage sludge from membrane bioreactors. |
| Abstract | Microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2-D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-D protein patterns obtained from different sludges collected from membrane bioreactors showed - besides common spots - significant differences. Selected proteins were identified with nano- HPLC-ESI-MS/MS. All membrane biological reactor (MBR) sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia-oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacterium Saprospira grandis in MBR1 was surprising. |
| Source | Proteomics (Weinh., Print) 11, pp. 2738–2744 |
| Keywords | De novo sequencingMembrane bioreactorMetaproteomicsMicrobial communitiesMicrobiologyProtein extraction |
| Journal | Proteomics (Weinh., Print) |
| Editor | Wiley-VCH-Verl., Weinheim, Germania |
| Year | 2011 |
| Type | Articolo in rivista |
| DOI | 10.1002/pmic.201000590 |
| Authors | Kuhn R., Benndorf D., Rapp E., Reichl U., Palese L.L., Pollice A. |
| Text | 42420 2011 10.1002/pmic.201000590 De novo sequencing Membrane bioreactor Metaproteomics Microbial communities Microbiology Protein extraction Metaproteome analysis of sewage sludge from membrane bioreactors. Kuhn R., Benndorf D., Rapp E., Reichl U., Palese L.L., Pollice A. BTU Cottbus, Department of Biotechnology of Wastewater Treatment, Cottbus, Germany Otto von Guericke University, Bioprocess Engineering, Magdeburg, Germany Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Magdeburg, Germany Department of Medical Biochemistry, Biology and Physics, University of Bari, Policlinico, Bari, Italy Microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2 D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2 D protein patterns obtained from different sludges collected from membrane bioreactors showed besides common spots significant differences. Selected proteins were identified with nano HPLC ESI MS/MS. All membrane biological reactor MBR sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacterium Saprospira grandis in MBR1 was surprising. 11 Published version Articolo in rivista Wiley VCH Verl. 1615 9853 Proteomics Weinh., Print Proteomics Weinh., Print Proteomics Weinh., Print Proteomics. Weinh., Print KUHN RAMONA alfieri.pollice POLLICE ALFIERI TA.P07.002.004 Processi biotecnologici e processi a membrana |