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TitleAnaerobic phenol biodegradation: kinetic study and microbial community shifts under high-concentration dynamic loading
AbstractThe anaerobic biodegradation of phenol has been realised in a sequencing batch reactor (SBR) under anaerobic conditions with phenol as sole carbon and energy source and with glucose as co-substrate. A step-change increase of phenol loading (from 100 up to 2000 mg/L of phenol concentration in the feed solution) has been applied during the acclimation phase in order to progressively induce the development of a specialised microbial consortium. This approach, combined with the dynamic sequence of operations characterising SBRs and with the high biomass retention time, led to satisfactory phenol and COD removal efficiencies with values > 70% for the highest phenol input (2000 mg/L) fed as the single carbon and energy source. Analysis of removal efficiencies and biodegradation rates suggested that the use of glucose as co-substrate did not induce a significant improvement in process performance. Kinetic tests have been performed at different initial phenol (400-1000 mg/L) and glucose (1880-0 mg/L) concentrations to kinetically characterise the developed biomass: estimated kinetic constants are suitable for application and no inhibitory effect due to high concentrations of phenol has been observed in all investigated conditions. The microbial community has been characterised at different operating conditions through molecular tools: results confirm the successful adaptation-operation approach of the microbial consortium showing a gradual increase in richness and diversity and the occurrence and selection of a high proportion of phenol-degrading genera at the end of the experimentation.Key Pointso Anaerobic phenol removal in the range of 70-99% in a sequencing batch reactor.o Negligible effect of co-substrate on removal efficiencies and biodegradation rates.o No biomass inhibition due to phenol concentration in the range of 400-1000 mg/L.o Increasing phenol loads promoted the culture enrichment of phenol-degrading genera.
SourceApplied microbiology and biotechnology
KeywordsPhenolAnaerobic biodegradationKinetic studyMicrobial characterisationInhibition. Acclimation
JournalApplied microbiology and biotechnology
EditorSpringer, Heidelberg ;, Germania
Year2020
TypeArticolo in rivista
DOI10.1007/s00253-020-10696-8
AuthorsMosca Angelucci D.; Clagnan E.; Brusetti L.; Tomei M.C.
Text423919 2020 10.1007/s00253 020 10696 8 Scopus 2 s2.0 85085932405 Phenol Anaerobic biodegradation Kinetic study Microbial characterisation Inhibition . Acclimation Anaerobic phenol biodegradation kinetic study and microbial community shifts under high concentration dynamic loading Mosca Angelucci D.; Clagnan E.; Brusetti L.; Tomei M.C. Water Research Institute, C.N.R, Via Salaria km 29.300, CP 10, Monterotondo Stazione Rome , 00015, Water Research Institute, C.N.R, Via Salaria km 29.300, CP 10, 00015, Monterotondo Stazione Rome , Italy, , Italy; Faculty of Science and Technology, Free University of Bozen/Bolzano, Piazza Universita 5, Bolzano, 39100, Faculty of Science and Technology, Free University of Bozen/Bolzano, Piazza Universita 5, 39100, Bolzano, Italy, , Italy The anaerobic biodegradation of phenol has been realised in a sequencing batch reactor SBR under anaerobic conditions with phenol as sole carbon and energy source and with glucose as co substrate. A step change increase of phenol loading from 100 up to 2000 mg/L of phenol concentration in the feed solution has been applied during the acclimation phase in order to progressively induce the development of a specialised microbial consortium. This approach, combined with the dynamic sequence of operations characterising SBRs and with the high biomass retention time, led to satisfactory phenol and COD removal efficiencies with values > 70% for the highest phenol input 2000 mg/L fed as the single carbon and energy source. Analysis of removal efficiencies and biodegradation rates suggested that the use of glucose as co substrate did not induce a significant improvement in process performance. Kinetic tests have been performed at different initial phenol 400 1000 mg/L and glucose 1880 0 mg/L concentrations to kinetically characterise the developed biomass estimated kinetic constants are suitable for application and no inhibitory effect due to high concentrations of phenol has been observed in all investigated conditions. The microbial community has been characterised at different operating conditions through molecular tools results confirm the successful adaptation operation approach of the microbial consortium showing a gradual increase in richness and diversity and the occurrence and selection of a high proportion of phenol degrading genera at the end of the experimentation.Key Pointso Anaerobic phenol removal in the range of 70 99% in a sequencing batch reactor.o Negligible effect of co substrate on removal efficiencies and biodegradation rates.o No biomass inhibition due to phenol concentration in the range of 400 1000 mg/L.o Increasing phenol loads promoted the culture enrichment of phenol degrading genera. Published version http //www.scopus.com/record/display.url eid=2 s2.0 85085932405 origin=inward Articolo in rivista Springer 0175 7598 Applied microbiology and biotechnology Applied microbiology and biotechnology Appl. microbiol. biotechnol. Applied microbiology and biotechnology. Applied microbiology and biotechnology Print domenica.moscaangelucci MOSCA ANGELUCCI DOMENICA mariaconcetta.tomei TOMEI MARIA CONCETTA