Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | MICROBIAL ECOLOGY METHODS FOR ASSESSING THE EFFECTS OF XENOBIOTICS IN WATER AND SOIL ECOSYSTEMS |
| Abstract | Microbial communities are valuable indicators of the occurrence of disturbances due to exogenous physico-chemical stressors. The assessment of variations in microbial community structure is of basic importance to permit to evaluate the impact of an environmental stressor. Complex microbial communities may serve as ideal and ecologically relevant toxicity indicators. A number of microbiologically driven processes have been proposed to evaluate the effects of xenobiotics on ecosystems. Proteins, genes, metabolites, or lipids that, when expressed, present a pattern of molecular change in an organism in response to a specific environmental stressor, can be defined as environmental biomarkers. The number of techniques to study microbial communities has increased exponentially over the last 20 years and the advent of culture-independent methods, such as molecular biological techniques, has changed the view of microbial diversity. Among these techniques epifluorescence microscopy techniques, such as direct count of bacterial abundance (DAPI count) and viability (Live/Dead cell viability assay), and Fluorescence In Situ Hybridization (FISH) are very useful to study microbial communities directly from their natural environment. We report some examples of application of these techniques for evaluating the effects of some contaminants (pesticide and pharmaceuticals) to soil and water ecosystems. |
| Source | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 157 (S1-S2), pp. s56–s56 |
| Keywords | Fluorescence in situ Hybridizationenvoronmental samplessoli and water |
| Journal | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology |
| Editor | Elsevier Science,, New York, Stati Uniti d'America |
| Year | 2010 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.cbpa.2010.06.157 |
| Authors | BARRA CARACCIOLO A, GRENNI P |
| Text | 42384 2010 10.1016/j.cbpa.2010.06.157 Fluorescence in situ Hybridization envoronmental samples soli and water MICROBIAL ECOLOGY METHODS FOR ASSESSING THE EFFECTS OF XENOBIOTICS IN WATER AND SOIL ECOSYSTEMS BARRA CARACCIOLO A, GRENNI P BARRA CARACCIOLO A, GRENNI P IRSA CNR Via Salaria km 29,300 Monterotondo Roma Microbial communities are valuable indicators of the occurrence of disturbances due to exogenous physico chemical stressors. The assessment of variations in microbial community structure is of basic importance to permit to evaluate the impact of an environmental stressor. Complex microbial communities may serve as ideal and ecologically relevant toxicity indicators. A number of microbiologically driven processes have been proposed to evaluate the effects of xenobiotics on ecosystems. Proteins, genes, metabolites, or lipids that, when expressed, present a pattern of molecular change in an organism in response to a specific environmental stressor, can be defined as environmental biomarkers. The number of techniques to study microbial communities has increased exponentially over the last 20 years and the advent of culture independent methods, such as molecular biological techniques, has changed the view of microbial diversity. Among these techniques epifluorescence microscopy techniques, such as direct count of bacterial abundance DAPI count and viability Live/Dead cell viability assay , and Fluorescence In Situ Hybridization FISH are very useful to study microbial communities directly from their natural environment. We report some examples of application of these techniques for evaluating the effects of some contaminants pesticide and pharmaceuticals to soil and water ecosystems. 157 http //www.sciencedirect.com/science/article/pii/S1095643310003417 Articolo pubblicato Comparative_Biochemistry_and_Physiology_Part_A.pdf Articolo in rivista Elsevier Science, 1095 6433 Comparative biochemistry and physiology. Part A, Molecular integrative physiology Comparative biochemistry and physiology. Part A, Molecular integrative physiology Comp. biochem. physiol., Part A Mol. integr. physiol. Comparative biochemistry and physiology. Molecular and integrative physiology Molecular integrative physiology CBP. Part A, Molecular integrative physiology anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA TA.P04.005.007 Strumenti di mitigazione dello stress quali quantitativo per i sistemi idrici |