Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Flow cytometric analysis of benthic prokaryotes attached to sediment particles |
| Abstract | Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity. |
| Source | Journal of microbiological methods 79 (2), pp. 246–249 |
| Journal | Journal of microbiological methods |
| Editor | Elsevier Science Ireland, Shannon, Paesi Bassi |
| Year | 2009 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.mimet.2009.09.005 |
| Authors | AMALFITANO S., FAZI S., PUDDU A. |
| Text | 42342 2009 10.1016/j.mimet.2009.09.005 ISI Web of Science WOS 000271792600018 Flow cytometric analysis of benthic prokaryotes attached to sediment particles AMALFITANO S., FAZI S., PUDDU A. Water Research Institute IRSA CNR , Via Salaria Km 29.300 00016 Monterotondo Rome, Italy Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity. 79 FLOW CYTOMETRIC Amalfitano_2009_JMM_Cytometry.pdf Articolo in rivista Elsevier Science Ireland 0167 7012 Journal of microbiological methods Journal of microbiological methods J. microbiol. methods Journal of microbiological methods. stefano.fazi FAZI STEFANO stefano.amalfitano AMALFITANO STEFANO alberto.puddu PUDDU ALBERTO TA.P02.002.003 Ruolo di comunita microbiche nella degradazione della sostanza organica e nella circolazione del carbonio nei sistemi acquatici |