| Title | A NEW FLUORESCENT OLIGONUCLEOTIDE PROBE FOR IN SITU DETECTION OF S-TRIAZINE-DEGRADING RHODOCOCCUS WRATISLAVIENSIS IN CONTAMINATED GROUNDWATER AND SOIL SAMPLES |
| Abstract | A bacterial strain (FPA1) capable of using terbuthylazine, simazine, atrazine, 2-hydroxysimazine, deethylatrazine, isopropylamine or ethylamine as its sole carbon source was isolated from a shallow aquifer chronically contaminated with s-triazine herbicides. Based on its 16S rDNA sequence analysis, the strain FPA1 was identified as Rhodococcus wratislaviensis. The disappearance time of 50% of the initial terbuthylazine concentration in the presence of this strain (DT50) was 62 days. This strain was also able to mineralise the [Uring 14C] triazine-ring, albeit at a slow rate. A 16S rRNA target oligonucleotide probe (RhLu) was designed, and the FISH protocol was optimised, in order to detect R. wratislaviensis in striazine- contaminated sites. The RhLu probe gave a positive signal (expressed as % of total DAPI-positive cells) in both the groundwater (2.19 ±0.41%) and soil (2.10 ±0.96%) samples analysed. Using the RhLu probe, R. wratislaviensis can be readily detected, and its population dynamics can be easily monitored, in soil and in water ecosystems contaminated with s-triazine. To the best of our knowledge, this is the first report showing the isolation, from groundwater, of a bacterial strain able to degrade s-triazines. |
| Source | Water research (Oxf.) 43 (12), pp. 2999–3008 |
| Keywords | TerbuthylazineBiodegradationGroundwaterFISH Probe |
| Journal | Water research (Oxf.) |
| Editor | Pergamon Press., New York, Regno Unito |
| Year | 2009 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.watres.2009.04.022 |
| Authors | GRENNI P. (a); GIBELLO A. (b); BARRA CARACCIOLO A. (a); FAJARDO C. (b); NANDE M. (b); SACCA' M.L. (a); MARTINEZ-INIGO M.J. (c); CICCOLI R. (d); MARTIN M. (b) |
| Text | 42307 2009 10.1016/j.watres.2009.04.022 PubMed 19476963 ISI Web of Science WOS 000267580500006 Terbuthylazine Biodegradation Groundwater FISH Probe A NEW FLUORESCENT OLIGONUCLEOTIDE PROBE FOR IN SITU DETECTION OF S TRIAZINE DEGRADING RHODOCOCCUS WRATISLAVIENSIS IN CONTAMINATED GROUNDWATER AND SOIL SAMPLES GRENNI P. a ; GIBELLO A. b ; BARRA CARACCIOLO A. a ; FAJARDO C. b ; NANDE M. b ; SACCA M.L. a ; MARTINEZ INIGO M.J. c ; CICCOLI R. d ; MARTIN M. b a Water Research Institute National Research Council, Area della Ricerca di Roma 1 Montelibretti, via Salaria km 29,300, CP10 00016 Monterotondo, Rome, Italy; b Facultad de Veterinaria, Complutense University Avenida Puerta de Hierro s/n, 28040 Madrid, Spain; c Instituto Madrileno de Investigacion y Desarrollo Rural, Agrario y Alimentario IMIDRA , Finca El Encin Km 38,2 A II Apdo 127, 28800 Madrid, Spain; d ENEA Centro Ricerche Casaccia Via Anguillarese, Rome, Italy A bacterial strain FPA1 capable of using terbuthylazine, simazine, atrazine, 2 hydroxysimazine, deethylatrazine, isopropylamine or ethylamine as its sole carbon source was isolated from a shallow aquifer chronically contaminated with s triazine herbicides. Based on its 16S rDNA sequence analysis, the strain FPA1 was identified as Rhodococcus wratislaviensis. The disappearance time of 50% of the initial terbuthylazine concentration in the presence of this strain DT50 was 62 days. This strain was also able to mineralise the Uring 14C triazine ring, albeit at a slow rate. A 16S rRNA target oligonucleotide probe RhLu was designed, and the FISH protocol was optimised, in order to detect R. wratislaviensis in striazine contaminated sites. The RhLu probe gave a positive signal expressed as % of total DAPI positive cells in both the groundwater 2.19 ±0.41% and soil 2.10 ±0.96% samples analysed. Using the RhLu probe, R. wratislaviensis can be readily detected, and its population dynamics can be easily monitored, in soil and in water ecosystems contaminated with s triazine. To the best of our knowledge, this is the first report showing the isolation, from groundwater, of a bacterial strain able to degrade s triazines. 43 http //www.ncbi.nlm.nih.gov/pubmed/19476963 Articolo pubblicato WR_Rhodococcus_pubblicato.pdf Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA TA.P04.005.007 Strumenti di mitigazione dello stress quali quantitativo per i sistemi idrici |
