| Title | STRUCTURE ANALYSIS AND PERFORMANCE OF A MICROBIAL COMMUNITY FROM A CONTAMINATED AQUIFER INVOLVED IN THE COMPLETE REDUCTIVE DECHLORINATION OF 1,1,2,2-TETRACHLOROETHANE TO ETHENE |
| Abstract | An anaerobic microcosm set up with aquifer material from a 1,1,2,2-tetrachloroethane (TeCA) contaminated site and amended with butyrate showed a complete TeCA dechlorination to ethene. A structure analysis of the microbial community was performed by fluorescence in situ hybridization (FISH) with already available and on purpose designed probes from sequences retrieved through 16S rDNA clone library construction. FISH was chosen as identification tool to evaluate in situ whether the retrieved sequences belong to primary bacteria responsible for the biodegradative reactions. FISH probes identified up to 80% of total bacteria and revealed the absence or the marginal presence of known TeCA degraders and the abundance of two well-known H2-utilizing halorespiring bacteria, Sulfurospirillum (32.4 ± 8.6% of total bacteria) and Dehalococcoides spp. (14.8±2.8), thereby providing a strong indication of their involvement in the dechlorination processes. These results were supported by the kinetic and thermodynamic analysis which provided indications that hydrogen was the actual electron donor for TeCA dechlorination. The specific probes, developed in this study, for known dechlorinators (i.e., Geobacter, Dehalobacter, and Sulfurospirillum species) represent a valuable tool for any future in situ bioremediation study as well as a quick and specific investigation tool for tracking their distribution in the field. |
| Source | Biotechnology and bioengineering (Print) 100 (2), pp. 240–249 |
| Keywords | tetrachloroethanereductive dechlorinationfluorescence in situ hybridizationbioremediation |
| Journal | Biotechnology and bioengineering (Print) |
| Editor | Wiley [etc.], New York, Stati Uniti d'America |
| Year | 2008 |
| Type | Articolo in rivista |
| DOI | 10.1002/bit.21776 |
| Authors | ROSSETTI S. (1); AULENTA F. (2); MAJONE M. (2); CROCETTI G. (3); TANDOI V. (1) |
| Text | 42285 2008 10.1002/bit.21776 ISI Web of Science WOS 000255503200005 tetrachloroethane reductive dechlorination fluorescence in situ hybridization bioremediation STRUCTURE ANALYSIS AND PERFORMANCE OF A MICROBIAL COMMUNITY FROM A CONTAMINATED AQUIFER INVOLVED IN THE COMPLETE REDUCTIVE DECHLORINATION OF 1,1,2,2 TETRACHLOROETHANE TO ETHENE ROSSETTI S. 1 ; AULENTA F. 2 ; MAJONE M. 2 ; CROCETTI G. 3 ; TANDOI V. 1 1 Water Research Institute, IRSA CNR, Via Reno 1, 00198 Rome, Italy; 2 Department of Chemistry, Sapienza University of Rome, Rome, Italy; 3 Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane, Australia An anaerobic microcosm set up with aquifer material from a 1,1,2,2 tetrachloroethane TeCA contaminated site and amended with butyrate showed a complete TeCA dechlorination to ethene. A structure analysis of the microbial community was performed by fluorescence in situ hybridization FISH with already available and on purpose designed probes from sequences retrieved through 16S rDNA clone library construction. FISH was chosen as identification tool to evaluate in situ whether the retrieved sequences belong to primary bacteria responsible for the biodegradative reactions. FISH probes identified up to 80% of total bacteria and revealed the absence or the marginal presence of known TeCA degraders and the abundance of two well known H2 utilizing halorespiring bacteria, Sulfurospirillum 32.4 ± 8.6% of total bacteria and Dehalococcoides spp. 14.8±2.8 , thereby providing a strong indication of their involvement in the dechlorination processes. These results were supported by the kinetic and thermodynamic analysis which provided indications that hydrogen was the actual electron donor for TeCA dechlorination. The specific probes, developed in this study, for known dechlorinators i.e., Geobacter, Dehalobacter, and Sulfurospirillum species represent a valuable tool for any future in situ bioremediation study as well as a quick and specific investigation tool for tracking their distribution in the field. 100 Structure analysis and performance of a microbial community from a contaminated aquifer involved in the complete reductive dechlorination of 1,1,2,2 tetrachloroethane to ethane Rossetti_2008.pdf Articolo in rivista Wiley etc. 0006 3592 Biotechnology and bioengineering Print Biotechnology and bioengineering Print Biotechnol. bioeng. Print Biotechnology and bioengineering. Print Biotechnology bioengineering Print Combinatorial chemistry Print simona.rossetti ROSSETTI SIMONA valter.tandoi TANDOI VALTER TA.P07.014.005 Risanamento di acque di falda |
