| Title | MICROBIAL CHARACTERISATION OF POLYHYDROXYALKANOATES STORING POPULATIONS SELECTED UNDER DIFFERENT OPERATING CONDITIONS USING A CELL-SORTING RT-PCR APPROACH |
| Abstract | The identity of polyhydroxyalkanoates (PHA) storing bacteria selected under aerobic dynamic feeding conditions, using propionate as carbon source (reactor P), was determined by applying reverse transcriptase-polymerase chain reaction (RT-PCR) on micromanipulated cells and confirmed by fluorescence in situ hybridisation (FISH). Four genera, Amaricoccus, Azoarcus, Thauera and Paraccoccus were detected, the latter only rarely present. All the biomass was involved in PHA storage as shown by Nile Blue staining. By quantitative FISH, their specific amount was determined in this and two other systems using acetate as the carbon substrate (sequencing batch reactor [SBR] A and A1). SBR A and reactor P had the same sludge retention time (SRT, 10 days), while reactor A1 was operated with the SRT of 1 day and the double organic loading rate (OLR). Systems fed with acetate (41.1 +/- 2.2 and 49.4 +/- 1.4% total Bacteria, for A and A1, respectively) became enriched in Thauera independently on the SRT and OLR, while it was only present in a minor amount when propionate was used as a substrate (1.9 +/- 0.2% total Bacteria). Amaricoccus was present in both reactors operated at 10 days SRT, favoured in the one fed with propionate (61.4 +/- 1.9% total bacteria), and almost completely removed at the SRT of 1 day. Azoarcus cells were found in all the analysed systems (3.9 +/- 0.3, 23.3 +/- 1.5 and 45.9 +/- 1.5 for P, A and A1, respectively), while Paracoccus was scarcely present. |
| Source | Applied microbiology and biotechnology 78 (2), pp. 351–360 |
| Keywords | PHA production . Aerobic dynamic feeding . Mixed cultures . RT-PCR . FISH |
| Journal | Applied microbiology and biotechnology |
| Editor | Springer, Heidelberg ;, Germania |
| Year | 2008 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00253-007-1301-5 |
| Authors | LEMOS P.c (1); LEVANTESI C. (2); SERAFIM L.S. (1); ROSSETTI S. (2); REIS M.A.M. (1); TANDOI V. (2) |
| Text | 42276 2008 10.1007/s00253 007 1301 5 ISI Web of Science WOS 000252614200018 Scopus 2 s2.0 38649129394 PubMed 18193421 PHA production . Aerobic dynamic feeding . Mixed cultures . RT PCR . FISH MICROBIAL CHARACTERISATION OF POLYHYDROXYALKANOATES STORING POPULATIONS SELECTED UNDER DIFFERENT OPERATING CONDITIONS USING A CELL SORTING RT PCR APPROACH LEMOS P.c 1 ; LEVANTESI C. 2 ; SERAFIM L.S. 1 ; ROSSETTI S. 2 ; REIS M.A.M. 1 ; TANDOI V. 2 1 REQUIMTE/CQFB, Chemistry Department, FCT/UNL, 2829 516 Caparica, Portugal; 2 Water Research Institute, CNR, Via Reno 1, 00198 Roma, Italy The identity of polyhydroxyalkanoates PHA storing bacteria selected under aerobic dynamic feeding conditions, using propionate as carbon source reactor P , was determined by applying reverse transcriptase polymerase chain reaction RT PCR on micromanipulated cells and confirmed by fluorescence in situ hybridisation FISH . Four genera, Amaricoccus, Azoarcus, Thauera and Paraccoccus were detected, the latter only rarely present. All the biomass was involved in PHA storage as shown by Nile Blue staining. By quantitative FISH, their specific amount was determined in this and two other systems using acetate as the carbon substrate sequencing batch reactor SBR A and A1 . SBR A and reactor P had the same sludge retention time SRT, 10 days , while reactor A1 was operated with the SRT of 1 day and the double organic loading rate OLR . Systems fed with acetate 41.1 / 2.2 and 49.4 / 1.4% total Bacteria, for A and A1, respectively became enriched in Thauera independently on the SRT and OLR, while it was only present in a minor amount when propionate was used as a substrate 1.9 / 0.2% total Bacteria . Amaricoccus was present in both reactors operated at 10 days SRT, favoured in the one fed with propionate 61.4 / 1.9% total bacteria , and almost completely removed at the SRT of 1 day. Azoarcus cells were found in all the analysed systems 3.9 / 0.3, 23.3 / 1.5 and 45.9 / 1.5 for P, A and A1, respectively , while Paracoccus was scarcely present. 78 http //www.springerlink.com/content/372x671201ut71r3/ MUD=MP lemos et al applied microbiology biotechnology 2008 Articolo pubblicato lemos_applied_microbiology_and_biotechnology_2008_.pdf Articolo in rivista Springer 0175 7598 Applied microbiology and biotechnology Applied microbiology and biotechnology Appl. microbiol. biotechnol. Applied microbiology and biotechnology. Applied microbiology and biotechnology Print caterina.levantesi LEVANTESI CATERINA simona.rossetti ROSSETTI SIMONA valter.tandoi TANDOI VALTER TA.P07.002.002 Sviluppo di nuovi processi di controllo dell inquinamento idrico. |
