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DatoValore
TitleIMPROVED QUANTIFICATION OF DEHALOCOCCOIDES SPECIES BY FLUORESCENCE IN SITU HYBRIDIZATION AND CATALYZED REPORTER DEPOSITION
AbstractChlorinated ethenes contamination of soil and groundwater is a widespread problem in most industrialized countries. To date, there is a general consensus in the literature that members of the genus Dehalococcoides are required for complete dechlorination of these compounds. The availability of specific identification tools to track their distribution in the field is therefore a topic of particular relevance in environmental studies. These microorganisms have been successfully visualized by fluorescence in situ hybridization (FISH) in highly active dechlorinating cultures. However, FISH detection of Dehalococcoides under low activity conditions can be strongly hampered by their small cell size and low ribosome content. In this study, catalyzed reporter deposition (CARD)-FISH was employed as an alternative detection method. In a trichloroethene (TCE) dechlorinating enrichment culture, CARD-FISH, using proteinase K as a permeabilization pre-treatment, was found to be significantly superior to conventional FISH in terms of both microscopic visualization and quantification efficiency (about 30%). An application of this method on contaminated aquifer samples is also presented and discussed
SourceSystematic and applied microbiology (Print) 31 (1), pp. 62–67
KeywordsFISH; CARD-FISH; Dehalococcoides spp.; TCE dehalogenation
JournalSystematic and applied microbiology (Print)
EditorElsevier., Jena, Germania
Year2008
TypeArticolo in rivista
DOI10.1016/j.syapm.2007.11.001
AuthorsFAZI S. (a); AULENTA F. (b); MAJONE M. (b); ROSSETTI S. (a)
Text42270 2008 10.1016/j.syapm.2007.11.001 ISI Web of Science WOS 000254993600008 Scopus 2 s2.0 39949085767 FISH; CARD FISH; Dehalococcoides spp.; TCE dehalogenation IMPROVED QUANTIFICATION OF DEHALOCOCCOIDES SPECIES BY FLUORESCENCE IN SITU HYBRIDIZATION AND CATALYZED REPORTER DEPOSITION FAZI S. a ; AULENTA F. b ; MAJONE M. b ; ROSSETTI S. a a Water Research Institute, IRSA CNR, Via Reno 1, 00198 Rome, Italy b Department of Chemistry Stanislao Cannizzaro , Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy Chlorinated ethenes contamination of soil and groundwater is a widespread problem in most industrialized countries. To date, there is a general consensus in the literature that members of the genus Dehalococcoides are required for complete dechlorination of these compounds. The availability of specific identification tools to track their distribution in the field is therefore a topic of particular relevance in environmental studies. These microorganisms have been successfully visualized by fluorescence in situ hybridization FISH in highly active dechlorinating cultures. However, FISH detection of Dehalococcoides under low activity conditions can be strongly hampered by their small cell size and low ribosome content. In this study, catalyzed reporter deposition CARD FISH was employed as an alternative detection method. In a trichloroethene TCE dechlorinating enrichment culture, CARD FISH, using proteinase K as a permeabilization pre treatment, was found to be significantly superior to conventional FISH in terms of both microscopic visualization and quantification efficiency about 30% . An application of this method on contaminated aquifer samples is also presented and discussed 31 Improved quantification Fazi_SAM_08.pdf Articolo in rivista Elsevier. 0723 2020 Systematic and applied microbiology Print Systematic and applied microbiology Print Syst. appl. microbiol. Print Systematic and applied microbiology. Print simona.rossetti ROSSETTI SIMONA stefano.fazi FAZI STEFANO TA.P07.014.005 Risanamento di acque di falda TA.P02.002.003 Ruolo di comunita microbiche nella degradazione della sostanza organica e nella circolazione del carbonio nei sistemi acquatici