Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbiome changes and oxidative capability of an anaerobic PCB dechlorinating enrichment culture after oxygen exposure |
| Abstract | Marine sediments may represent a sink of persistent organic pollutants including polychlorinated biphenyls (PCBs), toxic compounds prone to reductive or oxidative biodegradation pathways depending on the degree of chlorination and the positions of the chlorine atoms on the biphenyl rings. Superficial marine sediments can be subjected to episodic sediment resuspension by boat traffic and wind action causing the exposure of the underlying anaerobic layer to oxygen. Under these dynamic conditions, a deeper knowledge of the adaptation capability of the autochthonous microbial communities towards severe changes of the reaction environment is required. Insights into the metabolic potential of sediment community members may contribute greatly to the definition of efficient and reliable in situ bioremediation strategies. In this study, an anaerobic PCB-dechlorinating microbial consortium, developed from the chronically polluted marine sediment of Mar Piccolo (Taranto, Italy), was used to evaluate the response of the sediment microbiome to the imposition of aerobic conditions after prolonged anaerobic incubation. Compared to the anaerobic control, a dramatic change in microbiome composition, with a marked increase of Alphaproteobacteria of up to 39.2 % of total operational taxonomic units (OTUs) was revealed by high-throughput 16S rRNA gene sequencing. Accordingly, a decrement of low chlorinated PCBs (up to 58.3 ± 7.5 % for PCB 18) and the concomitant appearance of genes coding for PCB-degrading biphenyl dioxygenase (bph) were observed at the end of the aerobic incubation, suggesting the occurrence of oxidative PCB biodegradation processes. |
| Source | New biotechnology (Print) 56, pp. 96–102 |
| Keywords | In situ bioremediationMarine sedimentMicrobiomePolychlorinated biphenyls |
| Journal | New biotechnology (Print) |
| Editor | Elsevier Life Science, Amsterdam, Paesi Bassi |
| Year | 2020 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.nbt.2019.12.004 |
| Authors | Matturro, Bruna; Mascolo, Giuseppe; Rossetti, Simona |
| Text | 422184 2020 10.1016/j.nbt.2019.12.004 Scopus 2 s2.0 85076833430 In situ bioremediation Marine sediment Microbiome Polychlorinated biphenyls Microbiome changes and oxidative capability of an anaerobic PCB dechlorinating enrichment culture after oxygen exposure Matturro, Bruna; Mascolo, Giuseppe; Rossetti, Simona Istituto di Ricerca Sulle Acque, Bari; Istituto di Ricerca sulle Acque, Italy Marine sediments may represent a sink of persistent organic pollutants including polychlorinated biphenyls PCBs , toxic compounds prone to reductive or oxidative biodegradation pathways depending on the degree of chlorination and the positions of the chlorine atoms on the biphenyl rings. Superficial marine sediments can be subjected to episodic sediment resuspension by boat traffic and wind action causing the exposure of the underlying anaerobic layer to oxygen. Under these dynamic conditions, a deeper knowledge of the adaptation capability of the autochthonous microbial communities towards severe changes of the reaction environment is required. Insights into the metabolic potential of sediment community members may contribute greatly to the definition of efficient and reliable in situ bioremediation strategies. In this study, an anaerobic PCB dechlorinating microbial consortium, developed from the chronically polluted marine sediment of Mar Piccolo Taranto, Italy , was used to evaluate the response of the sediment microbiome to the imposition of aerobic conditions after prolonged anaerobic incubation. Compared to the anaerobic control, a dramatic change in microbiome composition, with a marked increase of Alphaproteobacteria of up to 39.2 % of total operational taxonomic units OTUs was revealed by high throughput 16S rRNA gene sequencing. Accordingly, a decrement of low chlorinated PCBs up to 58.3 ± 7.5 % for PCB 18 and the concomitant appearance of genes coding for PCB degrading biphenyl dioxygenase bph were observed at the end of the aerobic incubation, suggesting the occurrence of oxidative PCB biodegradation processes. 56 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85076833430 origin=inward Articolo in rivista Elsevier Life Science 1871 6784 New biotechnology Print New biotechnology Print New biotechnology. Print simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA giuseppe.mascolo MASCOLO GIUSEPPE |