Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | FILAMENTOUS ALFA-PROTEOBACTERIA ASSOCIATED WITH BULKINGIN INDUSTRIAL WASTEWATER TREATMENT PLANTS |
| Abstract | The phylogeny and distribution of filamentous Alphaproteobacteria, morphologically similar to "Nostocoida limicola" and Eikelboom Type 021N that cause the solids separation problem of bulking in industrial activated sludge plants is described here. A combination of culture-dependent and culture-independent molecular methods has characterized 5 novel species. 16S rRNA targeted oligonucleotide probes were designed for their in situ identification by fluorescence in situ hybridisation (FISH) and used to monitor their presence in 86 WWTPs treating different industrial effluents in four European countries. The involvement of these bacteria in bulking in these plants was confirmed. Filaments hybridising with the ALF-968 probe for the Alphaproteobacteria were present in 65% of the WWTPs examined. They were dominant and therefore probably responsible for bulking in 25.5% of them. The heterogeneous filamentous alphaproteobacterial populations in these communities could be completely identified after application of the oligonucleotide probes used in this study in 91% of the plants containing them. The only filamentous Alphaproteobacteria retrieved in pure culture was isolated from three different industrial WWTPs plants. None of these isolates could grow anaerobically on glucose or denitrify, but all grew aerobically and heterotrophically on a range of carbon sources. Although morphologically similar to the Eikelboom Type 021N morphotype, they were not involved in sulphur metabolism. These bacteria accumulated lipidic storage granules that were associated with their presence under the unbalanced growth conditions existing in these plants. |
| Source | Systematic and applied microbiology (Print) 27(6), pp. 716–727 |
| Keywords | Alphaproteobacteriaactivated sludgeindustrial WWTPsfluorescence in situ hybridization (FISH)bulking |
| Journal | Systematic and applied microbiology (Print) |
| Editor | Elsevier., Jena, Germania |
| Year | 2004 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1462-2920.2006.01046.x |
| Authors | LEVANTESI C. (1); BEIMFOHR C. (2); GEURKINK B. (3); ROSSETTI S. (1); THELEN K. (2); KROONEMAN J. (3); SNAIDR J. (2); VAN DER WAARDE J. (3); TANDOI V. (1) |
| Text | 42216 2004 10.1111/j.1462 2920.2006.01046.x ISI Web of Science WOS 000225647700013 Scopus 2 s2.0 7444226622 PubMed 15612630 Alphaproteobacteria activated sludge industrial WWTPs fluorescence in situ hybridization FISH bulking FILAMENTOUS ALFA PROTEOBACTERIA ASSOCIATED WITH BULKINGIN INDUSTRIAL WASTEWATER TREATMENT PLANTS LEVANTESI C. 1 ; BEIMFOHR C. 2 ; GEURKINK B. 3 ; ROSSETTI S. 1 ; THELEN K. 2 ; KROONEMAN J. 3 ; SNAIDR J. 2 ; VAN DER WAARDE J. 3 ; TANDOI V. 1 1 CNR, Water Research Institute, Rome, Italy; 2 Vermicon AG, Munich, Germany; 3 Bioclear B.V., Groningen, The Netherlands The phylogeny and distribution of filamentous Alphaproteobacteria, morphologically similar to Nostocoida limicola and Eikelboom Type 021N that cause the solids separation problem of bulking in industrial activated sludge plants is described here. A combination of culture dependent and culture independent molecular methods has characterized 5 novel species. 16S rRNA targeted oligonucleotide probes were designed for their in situ identification by fluorescence in situ hybridisation FISH and used to monitor their presence in 86 WWTPs treating different industrial effluents in four European countries. The involvement of these bacteria in bulking in these plants was confirmed. Filaments hybridising with the ALF 968 probe for the Alphaproteobacteria were present in 65% of the WWTPs examined. They were dominant and therefore probably responsible for bulking in 25.5% of them. The heterogeneous filamentous alphaproteobacterial populations in these communities could be completely identified after application of the oligonucleotide probes used in this study in 91% of the plants containing them. The only filamentous Alphaproteobacteria retrieved in pure culture was isolated from three different industrial WWTPs plants. None of these isolates could grow anaerobically on glucose or denitrify, but all grew aerobically and heterotrophically on a range of carbon sources. Although morphologically similar to the Eikelboom Type 021N morphotype, they were not involved in sulphur metabolism. These bacteria accumulated lipidic storage granules that were associated with their presence under the unbalanced growth conditions existing in these plants. 27 6 levantesi et al Systematic Applied Microbiology 2004 articolo pubblicato levantesi_systematic_and_applied_microbiology_2004_.pdf Articolo in rivista Elsevier. 0723 2020 Systematic and applied microbiology Print Systematic and applied microbiology Print Syst. appl. microbiol. Print Systematic and applied microbiology. Print simona.rossetti ROSSETTI SIMONA caterina.levantesi LEVANTESI CATERINA valter.tandoi TANDOI VALTER |