Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | IN SITU ANALYSIS OF NATIVE MICROBIAL COMMUNITIES IN COMPLEX SAMPLES WITH HIGH PARTICULATE LOADS |
| Abstract | In the present study a procedure combining a cell extraction method and Fluorescence In Situ Hybridization (FISH)for molecular monitoring and quanti fication of bacteria in soil and aquifer samples is presented.FISH was applied to bacterial cells extracted from the matrix by density gradient centrifugation.This separation method was applied to soil and aquifer samples and produced high cell recovery of 76.5%±4.4 and 78.0%±3.2,respectively.FISH,performed on the harvested cells,permitted a perfect visualization and quanti fication of bacteria.This approach is therefore promising for in situ detection of indigenous bacterial communities in complex samples. |
| Source | FEMS microbiology letters 253, pp. 55–58 |
| Journal | FEMS microbiology letters |
| Editor | Elsevier/North-Holland, Amsterdam, Paesi Bassi |
| Year | 2005 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.femsle.2005.09.018 |
| Authors | BARRA CARACCIOLO A.; GRENNI P.; CUPO C.; ROSSETTI S. |
| Text | 42161 2005 10.1016/j.femsle.2005.09.018 PubMed 16213678 ISI Web of Science WOS 000233366900007 IN SITU ANALYSIS OF NATIVE MICROBIAL COMMUNITIES IN COMPLEX SAMPLES WITH HIGH PARTICULATE LOADS BARRA CARACCIOLO A.; GRENNI P.; CUPO C.; ROSSETTI S. Water Research Institute, CNR, Rome, Italy In the present study a procedure combining a cell extraction method and Fluorescence In Situ Hybridization FISH for molecular monitoring and quanti fication of bacteria in soil and aquifer samples is presented.FISH was applied to bacterial cells extracted from the matrix by density gradient centrifugation.This separation method was applied to soil and aquifer samples and produced high cell recovery of 76.5%±4.4 and 78.0%±3.2,respectively.FISH,performed on the harvested cells,permitted a perfect visualization and quanti fication of bacteria.This approach is therefore promising for in situ detection of indigenous bacterial communities in complex samples. 253 http //www.ncbi.nlm.nih.gov/pubmed/16213678 Articolo pubblicato FEMSLetter2005.pdf Articolo in rivista Elsevier/North Holland 0378 1097 FEMS microbiology letters FEMS microbiology letters FEMS microbiol. lett. FEMS microbiology letters. Federation of European Microbiological Societies microbiology letters CUPO CINZIA paola.grenni GRENNI PAOLA simona.rossetti ROSSETTI SIMONA anna.barracaracciolo BARRA CARACCIOLO ANNA TA.P04.005.005 Gestione integrata delle informazioni ambientali |