Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE TO DETECT SIMAZINE DEGRADING BACTERIA IN SOIL SAMPLES |
| Abstract | We propose a new approach to evaluate the natural attenuation capacity of soil by using fluorescence in situ hybridization (FISH). A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s-triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol. Two agricultural soils (Lodi and Henares) with a history of simazine treatments, and two natural soils (Soto and Monza), without previous exposure to simazine, were studied. AtzB1 probe-target cells were found only in the agricultural soils and, in a greater percentage, in the Lodi soil, compared to the Henares one. Moreover, the greatest percentage of AtzB1 probe-target cells in Lodi was accompanied by a greater mineralization rate, compared to the Henares soil. The FISH method used in this study was suitable for the detection of simazine-degrading bacteria and could be a useful indicator of the potential of soil bioremediation. |
| Source | Chemosphere 71, pp. 703–710 |
| Journal | Chemosphere |
| Editor | Elsevier, Oxford, Regno Unito |
| Year | 2008 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.chemosphere.2007.10.071 |
| Authors | MARTIN M. (a); GIBELLO A. (a); LOBO C. (b); NANDE M.(a); GARBI C.(a); FAJARDO C. (a); BARRA CARACCIOLO A. (c); GRENNI P. (c); MARTINEZ INIGO M.J. (b) |
| Text | 42141 2008 10.1016/j.chemosphere.2007.10.071 ISI Web of Science WOS 000254793600012 APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE TO DETECT SIMAZINE DEGRADING BACTERIA IN SOIL SAMPLES MARTIN M. a ; GIBELLO A. a ; LOBO C. b ; NANDE M. a ; GARBI C. a ; FAJARDO C. a ; BARRA CARACCIOLO A. c ; GRENNI P. c ; MARTINEZ INIGO M.J. b a Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain b Instituto Madrileno de Investigacion y Desarrollo Rural, Agrario y Alimentario IMIDRA ,Finca El Enc n Km 38,2 A II Apdo 127, 28800 Madrid, Spain c Water Research Institute, National Research Council Via Reno, 1 00198 Rome, Italy We propose a new approach to evaluate the natural attenuation capacity of soil by using fluorescence in situ hybridization FISH . A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol. Two agricultural soils Lodi and Henares with a history of simazine treatments, and two natural soils Soto and Monza , without previous exposure to simazine, were studied. AtzB1 probe target cells were found only in the agricultural soils and, in a greater percentage, in the Lodi soil, compared to the Henares one. Moreover, the greatest percentage of AtzB1 probe target cells in Lodi was accompanied by a greater mineralization rate, compared to the Henares soil. The FISH method used in this study was suitable for the detection of simazine degrading bacteria and could be a useful indicator of the potential of soil bioremediation. 71 Articolo pubblicato ChemosphereAtzB2008.pdf Articolo in rivista Elsevier 0045 6535 Chemosphere Chemosphere Chemosphere Chemosphere. Chemosphere. Environmental chemistry, Chemosphere. Persistent organic pollutants and dioxins, Chemosphere. Environmental toxicology and risk assessment, Chemosphere. Science for Environmental toxicology, paola.grenni GRENNI PAOLA anna.barracaracciolo BARRA CARACCIOLO ANNA TA.P04.005.007 Strumenti di mitigazione dello stress quali quantitativo per i sistemi idrici |