| Title | UPTAKE AND ELIMINATION OF 4-NONYLPHENOL BY THE CLAM TAPES PHILIPPINARUM |
| Abstract | The present study is the first dealing with the accumulation and elimination of 4-nonylphenol (NP) by the Manila clam, Tapes philippinarum. Specimens of T. philippinarum were exposed to NP-spiked seawater, and the NP contents in whole soft tissue, gills, digestive gland, and haemolymph were measured. Actual NP concentrations in seawater and microalgae (Isochrysis galbana) used for feeding were also determined, and the algal bioconcentration factor (BCF) value (640 ml/g) was calculated. Volatilisation was the main cause of dissipation of NP from experimental tanks, attaining up to 78% of the NP added. NP accumulated by algae used for feeding was negligible with respect to the total added NP, and we concluded that in our experiments, clams accumulated NP mainly from water and not food intake. Accumulation followed a two-compartment, first-order rate coefficient model, with an uptake rate coefficient of 13.8 +/- 0.6 mL g(-1)h(-1)(fresh weight [fw]) and an elimination coefficient of 0.0070 +/- 0.0005 h(-1). Ninety percent of the steady state was reached after 14 days of exposure, and the BCF value at the steady state was 1958 +/- 158 mL g(-1) fw (1.8 +/- 0.2.105 based on lipid weight). Slightly less than 50% of NP bioaccumulated through water was allocated into the gills, whereas the rest was found in the digestive gland. In the first 8 hours, clams eliminated 51% of the NP accumulated, and only 2% of the NP accumulated was detected in the clams at the end of the elimination phase (day 14). Two-compartment first-order decay model described the elimination of the accumulated NP by considering the clam as two compartments each with a different elimination rate. The sudden elimination of NP in the initial hours can be attributed to the elimination of NP accumulated into the gills and readily available for excretion (k (e) = 0.30 +/- 0.07 h(-1)). The slower step of the elimination process should be the mobilisation of NP accumulated in internal organs, which must be carried into the haemolymph for excretion (k (e) = 0.0091 +/- 0.0002 h(-1)). Because T. philippinarum has been demonstrated to accumulate NP dissolved in water, high NP levels can be hypothesised in clams from highly contaminated environments. This research was performed according to all national and international guidelines for animal welfare. |
| Source | Archives of environmental contamination and toxicology (Print) 53, pp. 571–578 |
| Journal | Archives of environmental contamination and toxicology (Print) |
| Editor | Springer., New York, Stati Uniti d'America |
| Year | 2007 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00244-006-0250-9 |
| Authors | LIETTI E., MARIN M.G., MATOZZO V., POLESELLO S., VALSECCHI S. |
| Text | 42139 2007 10.1007/s00244 006 0250 9 ISI Web of Science WOS 000250138000008 UPTAKE AND ELIMINATION OF 4 NONYLPHENOL BY THE CLAM TAPES PHILIPPINARUM LIETTI E., MARIN M.G., MATOZZO V., POLESELLO S., VALSECCHI S. LIETTI E., POLESELLO S., VALSECCHI S. CNR, Water Res Inst, I 20047 Milan, Italy MARIN M.G., MATOZZO V. Univ Padua, Dept Biol, I 35131 Padua, Italy The present study is the first dealing with the accumulation and elimination of 4 nonylphenol NP by the Manila clam, Tapes philippinarum. Specimens of T. philippinarum were exposed to NP spiked seawater, and the NP contents in whole soft tissue, gills, digestive gland, and haemolymph were measured. Actual NP concentrations in seawater and microalgae Isochrysis galbana used for feeding were also determined, and the algal bioconcentration factor BCF value 640 ml/g was calculated. Volatilisation was the main cause of dissipation of NP from experimental tanks, attaining up to 78% of the NP added. NP accumulated by algae used for feeding was negligible with respect to the total added NP, and we concluded that in our experiments, clams accumulated NP mainly from water and not food intake. Accumulation followed a two compartment, first order rate coefficient model, with an uptake rate coefficient of 13.8 / 0.6 mL g 1 h 1 fresh weight fw and an elimination coefficient of 0.0070 / 0.0005 h 1 . Ninety percent of the steady state was reached after 14 days of exposure, and the BCF value at the steady state was 1958 / 158 mL g 1 fw 1.8 / 0.2.105 based on lipid weight . Slightly less than 50% of NP bioaccumulated through water was allocated into the gills, whereas the rest was found in the digestive gland. In the first 8 hours, clams eliminated 51% of the NP accumulated, and only 2% of the NP accumulated was detected in the clams at the end of the elimination phase day 14 . Two compartment first order decay model described the elimination of the accumulated NP by considering the clam as two compartments each with a different elimination rate. The sudden elimination of NP in the initial hours can be attributed to the elimination of NP accumulated into the gills and readily available for excretion k e = 0.30 / 0.07 h 1 . The slower step of the elimination process should be the mobilisation of NP accumulated in internal organs, which must be carried into the haemolymph for excretion k e = 0.0091 / 0.0002 h 1 . Because T. philippinarum has been demonstrated to accumulate NP dissolved in water, high NP levels can be hypothesised in clams from highly contaminated environments. This research was performed according to all national and international guidelines for animal welfare. 53 articolo Liettietal2007ArchivEnvTox_Clam.pdf Articolo in rivista Springer. 0090 4341 Archives of environmental contamination and toxicology Print Archives of environmental contamination and toxicology Print Arch. environ. contam. toxicol. Print stefano.polesello POLESELLO STEFANO saramaria.valsecchi VALSECCHI SARA MARIA TA.P03.011.004 Macrocostituenti inorganici ed inquinanti organici emergenti nel ciclo idrologico in aree remote e densamente abitate |
