Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum |
| Abstract | Background Oleaginous yeasts are able to accumulate very high levels of neutral lipids especially under condition of excess of carbon and nitrogen limitation (medium with high C/N ratio). This makes necessary the use of two-steps processes in order to achieve high level of biomass and lipid. To simplify the process, the decoupling of lipid synthesis from nitrogen starvation, by establishing a cytosolic acetyl-CoA formation pathway alternative to the one catalysed by ATP-citrate lyase, can be useful. Results In this work, we introduced a new cytoplasmic route for acetyl-CoA (AcCoA) formation in Rhodosporidium azoricum by overexpressing genes encoding for homologous phosphoketolase (Xfpk) and heterologous phosphotransacetylase (Pta). The engineered strain PTAPK4 exhibits higher lipid content and produces higher lipid concentration than the wild type strain when it was cultivated in media containing different C/N ratios. In a bioreactor process performed on glucose/xylose mixture, to simulate an industrial process for lipid production from lignocellulosic materials, we obtained an increase of 89% in final lipid concentration by the engineered strain in comparison to the wild type. This indicates that the transformed strain can produce higher cellular biomass with a high lipid content than the wild type. The transformed strain furthermore evidenced the advantage over the wild type in performing this process, being the lipid yields 0.13 and 0.05, respectively. Conclusion Our results show that the overexpression of homologous Xfpk and heterologous Pta activities in R. azoricum creates a new cytosolic AcCoA supply that decouples lipid production from nitrogen starvation. This metabolic modification allows improving lipid production in cultural conditions that can be suitable for the development of industrial bioprocesses using lignocellulosic hydrolysates. |
| Source | Microbial cell factories 18 (1) |
| Keywords | Rhodosporidium azoricumLipid productionOleaginous yeastsPhosphoketolasesPhosphotransacetylaseLignocellulosic hydrolysatesRenewable resources |
| Journal | Microbial cell factories |
| Editor | BioMed Central,, London, Regno Unito |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1186/s12934-019-1250-6 |
| Authors | Donzella, Silvia; Cucchetti, Daniela; Capusoni, Claudia; Rizzi, Aurora; Galafassi, Silvia; Chiara, Gambaro; Compagno, Concetta |
| Text | 421128 2019 10.1186/s12934 019 1250 6 ISI Web of Science WOS 000497380200002 Rhodosporidium azoricum Lipid production Oleaginous yeasts Phosphoketolases Phosphotransacetylase Lignocellulosic hydrolysates Renewable resources Engineering cytoplasmic acetyl CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum Donzella, Silvia; Cucchetti, Daniela; Capusoni, Claudia; Rizzi, Aurora; Galafassi, Silvia; Chiara, Gambaro; Compagno, Concetta Univ Milan; Versalis SPA; Eni SpA; CNR Background Oleaginous yeasts are able to accumulate very high levels of neutral lipids especially under condition of excess of carbon and nitrogen limitation medium with high C/N ratio . This makes necessary the use of two steps processes in order to achieve high level of biomass and lipid. To simplify the process, the decoupling of lipid synthesis from nitrogen starvation, by establishing a cytosolic acetyl CoA formation pathway alternative to the one catalysed by ATP citrate lyase, can be useful. Results In this work, we introduced a new cytoplasmic route for acetyl CoA AcCoA formation in Rhodosporidium azoricum by overexpressing genes encoding for homologous phosphoketolase Xfpk and heterologous phosphotransacetylase Pta . The engineered strain PTAPK4 exhibits higher lipid content and produces higher lipid concentration than the wild type strain when it was cultivated in media containing different C/N ratios. In a bioreactor process performed on glucose/xylose mixture, to simulate an industrial process for lipid production from lignocellulosic materials, we obtained an increase of 89% in final lipid concentration by the engineered strain in comparison to the wild type. This indicates that the transformed strain can produce higher cellular biomass with a high lipid content than the wild type. The transformed strain furthermore evidenced the advantage over the wild type in performing this process, being the lipid yields 0.13 and 0.05, respectively. Conclusion Our results show that the overexpression of homologous Xfpk and heterologous Pta activities in R. azoricum creates a new cytosolic AcCoA supply that decouples lipid production from nitrogen starvation. This metabolic modification allows improving lipid production in cultural conditions that can be suitable for the development of industrial bioprocesses using lignocellulosic hydrolysates. 18 Published version Articolo in rivista BioMed Central, 1475 2859 Microbial cell factories Microbial cell factories Microb. cell fact. Microbial cell factories silvia.galafassi GALAFASSI SILVIA |