| Title | VITELLOGENIN INDUCTION IN THE CLAM TAPES PHYLIPPINARUM AFTER EXPOSURE TO 4-NONYLPHENOL |
| Abstract | Nonylphenol (NP) is reported to exert estrogenic effects in marine organisms, being able to mimic the action of endogenous estrogens. As a consequence, NP can induce vitellogenin (Vg) synthesis, the major precursor of the egg-yolk proteins. In our previous study [Matozzo and Marin, 2005. Environ. Res., 97, 43-49], we found that exposure for 7 days of the clams Tapes philippinarum to NP caused a dose-related induction of Vg in both haemolymph and digestive gland of males. In the present study, Vg induction was evaluated in sexually undifferentiated clams exposed to NP: for 7 days to 0, 0 + acetone, 0.025, 0.05, 0.1, and 0.2 mg NP/L and for 14 days to 0, 0 + acetone, 0.025, 0.05, and 0.1 mg NP/L. Vg was determined in both haemolymph and digestive gland by the alkali-labile phosphate (ALP) assay. In the haemolymph Ca2+ levels were also measured. Exposure for 7 days to 0.2 mg NP/L resulted in significant increases (p < 0.05) in ALP in both aemolymph and digestive gland with respect to controls, whereas no difference was observed in Ca2+ levels. After 14 days exposure, ALP significantly (p < 0.05) increased in digestive gland from 0.05 and 0.1 mg NP/Lexposed clams. Increases (p < 0.05) in Ca2+ levels were recorded in haemolymph from clams exposed
to 0.025 and 0.05 mg NP/L. Results obtained demonstrates that NP induces Vg synthesis in T. philippinarum also far from the reproductive period. As endocrine disruption may cause fertility reduction and alteration in sex ratio, a condition of potential risk for clam populations in estuarine areas is highlighted. This study was supported by grants from Co.Ri.La (Second Research Programme, 2004-2006). |
| Source | Marine environmental research 62 (S1), pp. 232–233 |
| Journal | Marine environmental research |
| Editor | Applied Science Publishers,, Barking, Regno Unito |
| Year | 2006 |
| Type | Articolo in rivista |
| Authors | MATOZZO V., TOMEI A., VALSECCHI S., POLESELLO S., MARIN M.G. |
| Text | 42098 2006 VITELLOGENIN INDUCTION IN THE CLAM TAPES PHYLIPPINARUM AFTER EXPOSURE TO 4 NONYLPHENOL MATOZZO V., TOMEI A., VALSECCHI S., POLESELLO S., MARIN M.G. Department of Biology, University of Padova, Via U. Bassi, 58/B 35131 Padova, Italy; CNR IRSA, Via della Mornera, 25 20047 Brugherio MI , Italy Nonylphenol NP is reported to exert estrogenic effects in marine organisms, being able to mimic the action of endogenous estrogens. As a consequence, NP can induce vitellogenin Vg synthesis, the major precursor of the egg yolk proteins. In our previous study Matozzo and Marin, 2005. Environ. Res., 97, 43 49 , we found that exposure for 7 days of the clams Tapes philippinarum to NP caused a dose related induction of Vg in both haemolymph and digestive gland of males. In the present study, Vg induction was evaluated in sexually undifferentiated clams exposed to NP for 7 days to 0, 0 acetone, 0.025, 0.05, 0.1, and 0.2 mg NP/L and for 14 days to 0, 0 acetone, 0.025, 0.05, and 0.1 mg NP/L. Vg was determined in both haemolymph and digestive gland by the alkali labile phosphate ALP assay. In the haemolymph Ca2 levels were also measured. Exposure for 7 days to 0.2 mg NP/L resulted in significant increases p < 0.05 in ALP in both aemolymph and digestive gland with respect to controls, whereas no difference was observed in Ca2 levels. After 14 days exposure, ALP significantly p < 0.05 increased in digestive gland from 0.05 and 0.1 mg NP/Lexposed clams. Increases p < 0.05 in Ca2 levels were recorded in haemolymph from clams exposed to 0.025 and 0.05 mg NP/L. Results obtained demonstrates that NP induces Vg synthesis in T. philippinarum also far from the reproductive period. As endocrine disruption may cause fertility reduction and alteration in sex ratio, a condition of potential risk for clam populations in estuarine areas is highlighted. This study was supported by grants from Co.Ri.La Second Research Programme, 2004 2006 . 62 Articolo in rivista Applied Science Publishers, 0141 1136 Marine environmental research Marine environmental research Mar. environ. res. Marine environmental research. Journal of marine environmental research stefano.polesello POLESELLO STEFANO saramaria.valsecchi VALSECCHI SARA MARIA TA.P03.011.004 Macrocostituenti inorganici ed inquinanti organici emergenti nel ciclo idrologico in aree remote e densamente abitate |
