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TitleChAMBRe: A new atmospheric simulation chamber for aerosol modelling and bio-aerosol research
AbstractAtmospheric simulation chambers are exploratory platforms used to study various atmospheric processes at realistic but controlled conditions. We describe here a new facility specifically designed for the research on atmospheric bio-aerosol as well as the protocols to produce, inject, expose and collect bio-aerosols. ChAMBRe (Chamber for Aerosol Modelling and Bio-aerosol Research) is installed at the Physics Department of the University of Genoa, Italy, and it is a node of the EUROCHAMP-2020 consortium. The chamber is made of stainless steel with a total volume of about 2.2m3. The lifetime of aerosol particle with dimension from a few hundreds of nanometres to a few microns varies from about 2 to 10 h. Characteristic parts of the facility are the equipment and the procedures to grow, inject, and extract bacterial strains in the chamber volume while preserving their viability. Bacteria are part of the atmospheric ecosystem and have impact on several levels as: health related issues, cloud formation, and geochemistry. ChAMBRe will host experiments to study the bacterial viability vs. the air quality level, i.e. the atmospheric concentration of gaseous and aerosol pollutants. In this article, we report the results of the characterization tests as well as of the first experiments performed on two bacterial strains belonging to the Grampositive and Gram-negative groups. A reproducibility at the 10% level has been obtained in repeated injections and collection runs with a clean atmosphere, assessing this way the chamber sensitivity for systematic studies on bacterial viability vs. environmental conditions.
SourceAtmospheric measurement techniques (Print) 11, pp. 5885–5900
Keywordsatmospheric simulation chamber
JournalAtmospheric measurement techniques (Print)
EditorCopernicus Publications, Göttingen, Germania
Year2018
TypeArticolo in rivista
DOI10.5194/amt-11-5885-2018
AuthorsMassabo D.; Giulia Danelli S.; Brotto P.; Comite A.; Costa C.; Di Cesare A.; Francois Doussin J.; Ferraro F.; Formenti P.; Gatta E.; Negretti L.; Oliva M.; Parodi F.; Vezzulli L.; Prati P.
Text420958 2018 10.5194/amt 11 5885 2018 Scopus 2 s2.0 85055553906 atmospheric simulation chamber ChAMBRe A new atmospheric simulation chamber for aerosol modelling and bio aerosol research Massabo D.; Giulia Danelli S.; Brotto P.; Comite A.; Costa C.; Di Cesare A.; Francois Doussin J.; Ferraro F.; Formenti P.; Gatta E.; Negretti L.; Oliva M.; Parodi F.; Vezzulli L.; Prati P. Dipartimento di Fisica, Universita di Genova, via Dodecaneso 33, Genoa, 16146, , , Italy; Dipartimento di Fisica, Universita di Genova, via Dodecaneso 33, Genoa, 16146, , , Italy; INFN, via Dodecaneso 33, Genoa, 16146, , Italy; PMTEN Srl, Piazza della Vittoria 7/14, Genoa, 16121, , Italy; Dipartimento di Chimica e Chimica Industriale, Universita di Genova, via Dodecaneso 31, Genoa, 16146, , , Italy; Dipartimento di Chimica e Chimica Industriale, Universita di Genova, via Dodecaneso 31, Genoa, 16146, , , Italy; Dipartimento di Scienze della Terra dell Ambiente e della Vita, Universita di Genova, Corso Europa 26, Genoa, 16132, , , Italy; Dipartimento di Scienze della Terra dell Ambiente e della Vita, Universita di Genova, Corso Europa 26, Genoa, 16132, , , Italy; LISA, UMR, Universite Paris Est Creteil, Avenue du General de Gaulle, Creteil, 94000, , , , France; LISA, UMR, Universite Paris Est Creteil, Avenue du General de Gaulle, Creteil, 94000, , , , France; LISA, UMR, Universite Paris Est Creteil, Avenue du General de Gaulle, Creteil, 94000, , , , France Atmospheric simulation chambers are exploratory platforms used to study various atmospheric processes at realistic but controlled conditions. We describe here a new facility specifically designed for the research on atmospheric bio aerosol as well as the protocols to produce, inject, expose and collect bio aerosols. ChAMBRe Chamber for Aerosol Modelling and Bio aerosol Research is installed at the Physics Department of the University of Genoa, Italy, and it is a node of the EUROCHAMP 2020 consortium. The chamber is made of stainless steel with a total volume of about 2.2m3. The lifetime of aerosol particle with dimension from a few hundreds of nanometres to a few microns varies from about 2 to 10 h. Characteristic parts of the facility are the equipment and the procedures to grow, inject, and extract bacterial strains in the chamber volume while preserving their viability. Bacteria are part of the atmospheric ecosystem and have impact on several levels as health related issues, cloud formation, and geochemistry. ChAMBRe will host experiments to study the bacterial viability vs. the air quality level, i.e. the atmospheric concentration of gaseous and aerosol pollutants. In this article, we report the results of the characterization tests as well as of the first experiments performed on two bacterial strains belonging to the Grampositive and Gram negative groups. A reproducibility at the 10% level has been obtained in repeated injections and collection runs with a clean atmosphere, assessing this way the chamber sensitivity for systematic studies on bacterial viability vs. environmental conditions. 11 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85055553906 origin=inward Articolo in rivista Copernicus Publications 1867 1381 Atmospheric measurement techniques Print Atmospheric measurement techniques Print Atmos. meas. tech. Print Atmospheric measurement techniques. Print AMT Print Print andrea.dicesare DI CESARE ANDREA