| Title | Phylogeny, physiology and distribution of 'Candidatus Microthrix calida', a new Microthrix species isolated from industrial activated sludge wastewater treatment plants. |
| Abstract | Twelve strains of filamentous bacteria morphologically identified as 'Microthrix parvicella' were isolated from industrial activated sludge wastewater treatment plants. 16S rRNA gene sequences analysis showed that these strains were all closely related to 'Candidatus Microthrix parvicella'. Six of them, however, had a 16S rRNA gene similarity of only 95.7% and 96.7% to 'Candidatus Microthrix parvicella' suggesting the presence of a new species. The name 'Candidatus Microthrix calida' is proposed for this new microorganism. The physiological properties of these six isolates supported the description of a new taxon. The 'Candidatus Microthrix calida' strains produced thin filaments (0.3-0.7 microm diameter), they did not grow on the media supporting the growth of 'Candidatus Microthrix parvicella' and could be cultivated at higher temperature (up to 36.5 degrees C). Preliminary data on substrate uptake were obtained by microautoradiography on pure culture. Two new fluorescence in situ hybridization (FISH) probes, Mpa-T1-1260 specific for 'Candidatus Microthrix calida' and Mpa-all-1410 targeting both Microthrix species, were designed. The presence of Microthrix spp. was investigated in 114 activated sludge plants. 'Microthrix parvicella' morphotype was detected in 23% of the analysed samples and FISH analysis revealed that 'Candidatus Microthrix calida' was present in 5% of them. The remaining 'M. parvicella' filaments were positive with probe Mpa-all-1410 but could not all be identified as 'Candidatus Microthrix parvicella' suggesting the presence of more hitherto undescribed biodiversity within this morphotype. |
| Source | Environmental microbiology (Print) 8 (9), pp. 1552–1563 |
| Keywords | microthrixfilamentous bacteriabulkingactivated sludgeFISH |
| Journal | Environmental microbiology (Print) |
| Editor | Blackwell Science., Oxford, Regno Unito |
| Year | 2006 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1462-2920.2006.01046.x |
| Authors | LEVANTESI C. (1); ROSSETTI S. (1); THELEN K. (2); KRAGELUND C. (3); KROONEMAN J. (4); EIKELBOOM D. (5); NIELSEN H.P. (3); TANDOI V. (1) |
| Text | 42092 2006 10.1111/j.1462 2920.2006.01046.x ISI Web of Science WOS 000239510600007 Scopus 2 s2.0 33746776930 PubMed 16913916 microthrix filamentous bacteria bulking activated sludge FISH Phylogeny, physiology and distribution of Candidatus Microthrix calida , a new Microthrix species isolated from industrial activated sludge wastewater treatment plants. LEVANTESI C. 1 ; ROSSETTI S. 1 ; THELEN K. 2 ; KRAGELUND C. 3 ; KROONEMAN J. 4 ; EIKELBOOM D. 5 ; NIELSEN H.P. 3 ; TANDOI V. 1 1 CNR, Water Research Institute, Via Reno 1, 00198 Rome, Italy; 2 Vermicon AG, Emmy Noether Straße 2, 80992 Munich, Germany; 3 Section of Environmental Engineering, Department of Life Sciences, Aalborg University, Sohngaardsholmsvej 57, DK 9000 Aalborg, Denmark; 4 Bioclear B.V., Rozenburglaan 13, 9727 DL Groningen, the Netherlands; 5 TNO, Deventerweg 38, 7203AK, Zutphen, the Netherlands Twelve strains of filamentous bacteria morphologically identified as Microthrix parvicella were isolated from industrial activated sludge wastewater treatment plants. 16S rRNA gene sequences analysis showed that these strains were all closely related to Candidatus Microthrix parvicella . Six of them, however, had a 16S rRNA gene similarity of only 95.7% and 96.7% to Candidatus Microthrix parvicella suggesting the presence of a new species. The name Candidatus Microthrix calida is proposed for this new microorganism. The physiological properties of these six isolates supported the description of a new taxon. The Candidatus Microthrix calida strains produced thin filaments 0.3 0.7 microm diameter , they did not grow on the media supporting the growth of Candidatus Microthrix parvicella and could be cultivated at higher temperature up to 36.5 degrees C . Preliminary data on substrate uptake were obtained by microautoradiography on pure culture. Two new fluorescence in situ hybridization FISH probes, Mpa T1 1260 specific for Candidatus Microthrix calida and Mpa all 1410 targeting both Microthrix species, were designed. The presence of Microthrix spp. was investigated in 114 activated sludge plants. Microthrix parvicella morphotype was detected in 23% of the analysed samples and FISH analysis revealed that Candidatus Microthrix calida was present in 5% of them. The remaining M. parvicella filaments were positive with probe Mpa all 1410 but could not all be identified as Candidatus Microthrix parvicella suggesting the presence of more hitherto undescribed biodiversity within this morphotype. 8 http //onlinelibrary.wiley.com/doi/10.1111/j.1462 2920.2006.01046.x/abstract levantesi et al environmental microbiology 2006 articolo pubblicato levantesi_environmental_microbiology_2006_.pdf Articolo in rivista Blackwell Science. 1462 2912 Environmental microbiology Print Environmental microbiology Print Environ. microbiol. Print simona.rossetti ROSSETTI SIMONA caterina.levantesi LEVANTESI CATERINA valter.tandoi TANDOI VALTER TA.P07.002.002 Sviluppo di nuovi processi di controllo dell inquinamento idrico. |
