| Abstract | The beta lactamase gene bla, responsible of the resistance to cephalosporins, has been detected in microbes from hospitals to open waters. We studied the seasonality and stability of bla in Lake Maggiore over 3 years and the role of potential inputs of allochthonous bacteria and/or antibiotic pollution in promoting its occurrence. bla was mainly present from January to July in the pelagic microbial community and the gene occurrence was significantly related to low water temperature. To evaluate its temporal stability in the bacterial community over a short period, we measured bla daily over the course of 6 days. The gene was below the limit of quantification except for one sampling when its abundance peaked, suggesting a point contamination. The bacterial community of the lake in which bla was detected suggests that at least two distinct bacterial populations contained the gene. The occurrence of known bla containing genera and the occurrence of the gene, however, did not overlap. Furthermore, the experimental addition of cefotaxime to lake water incubations did not promote abundance of the gene. These data imply that bla was present in the environmental microbial community. Increases of gene abundances were likely caused by environmental parameters other than antibiotic contamination. |
| Text | 420911 2019 10.1007/s10750 019 04043 w Scopus 2 s2.0 85070351177 antibiotic resistance lake sediment Seasonality of the antibiotic resistance gene bla CTX M in temperate Lake Maggiore Eckert E.M.; Di Cesare A.; Malki L. S.; Villiger J.; Pernthaler J.; Callieri C.; Bertoni R.; Corno G. Microbial Ecology Group MEG , National Research Council of Italy Institute of Water Research CNR IRSA , Largo Tonolli 50, Verbania, 28921, Microbial Ecology Group MEG , National Research Council of Italy Institute of Water Research CNR IRSA , Largo Tonolli 50, 28921, Verbania, Italy, , Italy; Limnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Limnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland, , Switzerland The beta lactamase gene bla, responsible of the resistance to cephalosporins, has been detected in microbes from hospitals to open waters. We studied the seasonality and stability of bla in Lake Maggiore over 3 years and the role of potential inputs of allochthonous bacteria and/or antibiotic pollution in promoting its occurrence. bla was mainly present from January to July in the pelagic microbial community and the gene occurrence was significantly related to low water temperature. To evaluate its temporal stability in the bacterial community over a short period, we measured bla daily over the course of 6 days. The gene was below the limit of quantification except for one sampling when its abundance peaked, suggesting a point contamination. The bacterial community of the lake in which bla was detected suggests that at least two distinct bacterial populations contained the gene. The occurrence of known bla containing genera and the occurrence of the gene, however, did not overlap. Furthermore, the experimental addition of cefotaxime to lake water incubations did not promote abundance of the gene. These data imply that bla was present in the environmental microbial community. Increases of gene abundances were likely caused by environmental parameters other than antibiotic contamination. 843 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85070351177 origin=inward Seasonality of the antibiotic resistance gene bla CTX M in temperate Lake Maggiore Eckert et al. Hydrobiol 2019 TOP.pdf Articolo in rivista Kluwer Academic Publishers 0018 8158 Hydrobiologia The Hague. Print Hydrobiologia The Hague. Print Hydrobiologia The Hague. Print Hydrobiologia. The Hague. Print Hydrobiologia Dordrecht The Hague. Print Hydrobiologia Boston The Hague. Print Hydrobiologia London The Hague. Print roberto.bertoni BERTONI ROBERTO cristiana.callieri CALLIERI CRISTIANA gianluca.corno CORNO GIANLUCA andrea.dicesare DI CESARE ANDREA estermaria.eckert ECKERT ESTER MARIA |
