| Title | IDENTITY, ABUNDANCE AND ECOPHYSIOLOGY OF FILAMENTOUS CHLOROFLEXI SPECIES PRESENT IN ACTIVATED SLUDGE TREATMENT PLANTS |
| Abstract | Filamentous Chloroflexi species are often present in activated sludge wastewater treatment plants in relatively low numbers, although bulking incidences caused by Chloroflexi filaments have been observed. A new species-specific gene probe for FISH was designed and using phylum-, subdivision-, morphotype 1851- and species-specific gene probes, the abundance of Chloroflexi filaments were monitored in samples from 126 industrial wastewater treatment plants from five European countries. Chloroflexi filaments were present in 50% of the samples, although in low quantities. In most treatment plants the filaments could only be identified with phylum or subdivision probes, indicating the presence of great undescribed biodiversity. The ecophysiology of various Chloroflexi filaments was investigated by a suite of in situ methods. The experiments revealed that Chloroflexi constituted a specialized group of filamentous bacteria only active under aerobic conditions consuming primarily carbohydrates. Many exo-enzymes were excreted, e.g. chitinase, glucuronidase and galactosidase, suggesting growth on complex polysaccharides. The surface of Chloroflexi filaments appeared to be hydrophilic compared to other filaments present. These results are generally supported by physiological studies of two new isolates. Based on the results obtained in this study, the potential role of filamentous Chloroflexi species in activated sludge is discussed. |
| Source | FEMS microbiology, ecology (print) 59, pp. 671–682 |
| Keywords | Chloroflexifilamentous bacteriaactivated sludgeecophysiology. |
| Journal | FEMS microbiology, ecology (print) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2007 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1574-6941.2006.00251.x |
| Authors | KRAGELUND C. (1); LEVANTESI C. (2); BORGER A. (3); THELEN K. (4); EIKELBOOM D. (5); TANDOI V. (2); KONG Y. (1); VAN DER WAARDE J. (6); KROONEMAN J. (7); ROSSETTI S. (2); THOMSEN T.R. (1); NIELSEN P.H. (1) |
| Text | 42090 2007 10.1111/j.1574 6941.2006.00251.x ISI Web of Science WOS 000244719400013 PubMed 17381520 Scopus 2 s2.0 33847699407 Chloroflexi filamentous bacteria activated sludge ecophysiology. IDENTITY, ABUNDANCE AND ECOPHYSIOLOGY OF FILAMENTOUS CHLOROFLEXI SPECIES PRESENT IN ACTIVATED SLUDGE TREATMENT PLANTS KRAGELUND C. 1 ; LEVANTESI C. 2 ; BORGER A. 3 ; THELEN K. 4 ; EIKELBOOM D. 5 ; TANDOI V. 2 ; KONG Y. 1 ; VAN DER WAARDE J. 6 ; KROONEMAN J. 7 ; ROSSETTI S. 2 ; THOMSEN T.R. 1 ; NIELSEN P.H. 1 1 Section of Environmental Engineering, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark; 2 CNR, Water Res Institute, Rome, Italy; 3 TNO MEP; Department of Environmental Engineering; Laan van Apeldoorn, The Netherlands; 4 VERMICON AG, Munich, Germany; 5 ASIS vof, Zutphen, The Netherlands; 6 SNV Kameroen, Koeriersdienst Ministerie van Buitenlandse Zaken, Den Haag, The Netherlands; 7 BIOCLEAR Environmental Biotechnology, Groningen, The Netherlands Filamentous Chloroflexi species are often present in activated sludge wastewater treatment plants in relatively low numbers, although bulking incidences caused by Chloroflexi filaments have been observed. A new species specific gene probe for FISH was designed and using phylum , subdivision , morphotype 1851 and species specific gene probes, the abundance of Chloroflexi filaments were monitored in samples from 126 industrial wastewater treatment plants from five European countries. Chloroflexi filaments were present in 50% of the samples, although in low quantities. In most treatment plants the filaments could only be identified with phylum or subdivision probes, indicating the presence of great undescribed biodiversity. The ecophysiology of various Chloroflexi filaments was investigated by a suite of in situ methods. The experiments revealed that Chloroflexi constituted a specialized group of filamentous bacteria only active under aerobic conditions consuming primarily carbohydrates. Many exo enzymes were excreted, e.g. chitinase, glucuronidase and galactosidase, suggesting growth on complex polysaccharides. The surface of Chloroflexi filaments appeared to be hydrophilic compared to other filaments present. These results are generally supported by physiological studies of two new isolates. Based on the results obtained in this study, the potential role of filamentous Chloroflexi species in activated sludge is discussed. 59 http //onlinelibrary.wiley.com/doi/10.1111/j.1574 6941.2006.00251.x/abstract kragelund et al fems microbiology ecology 2007 articolo pubblicato kragelund_fems_microbiology_ecology_2007_.pdf Articolo in rivista Elsevier 0168 6496 FEMS microbiology, ecology print FEMS microbiology, ecology print FEMS microbiol. ecol. print FEMS microbiology, ecology. print Federation of European Microbiological Societies microbiology, ecology print simona.rossetti ROSSETTI SIMONA caterina.levantesi LEVANTESI CATERINA valter.tandoi TANDOI VALTER TA.P07.002.002 Sviluppo di nuovi processi di controllo dell inquinamento idrico. |
