Scheda di dettaglio – i prodotti della ricerca

DatoValore
TitleKinetic and phylogenetic characterization of an anaerobic dechlorinating microbial community
AbstractA laboratory scale sequencing batch reactor (SBR) operating for enhanced biological phosphorus removal (EBPR) and fed with a mixture of volatile fatty acids (VFAs) showed stable and efficient EBPR capacity over a four year period. Phosphorus (P), poly–b–hydroxyalkanoate (PHA) and glycogen cycling consistent with classical anaerobic/aerobic EBPR were demonstrated with the order of anaerobic VFA uptake being propionate, acetate then butyrate. The SBR was operated without pH control and 63.67 ± 13.86 mg P/L was released anaerobically. The P% of the sludge fluctuated between 6% and 10% over the operating period (average of 8.04 ± 1.31%). Four main morphological types of floc-forming bacteria were observed in the sludge during one year of intensive microscopic observation. Two of them were mainly responsible for anaerobic/aerobic P and PHA transformations. Fluorescence in situ hybridisation (FISH) and post-FISH chemical staining for intracellular polyphosphate and PHA were used to determine that “Candidatus Accumulibacter phosphatis” was the most abundant polyphosphate accumulating organism (PAO), forming large clusters of coccobacilli (1.0-1.5 µm) and comprising 53% of the sludge bacteria. Also by these methods, large coccobacillus-shaped gammaproteobacteria (2.5-3.5 µm) from a recently described novel cluster were glycogen accumulating organisms (GAOs) comprising 13% of the bacteria. Tetrad forming organisms (TFOs) consistent with the “G bacterium” morphotype were alphaproteobacteria, but not Amaricoccus spp., and comprised 25% of all bacteria. According to chemical staining, TFOs were occasionally able to store PHA anaerobically and utilise it aerobically
KeywordsEBPRPAOGAOactivated sludgeFISH
Year2003
TypeArticolo in rivista
AuthorsROSSETTI S., BLACKALL L.L., MAJONE M., HUGENHOLTZ P., PLUMB J., TANDOI V.
Text42070 2003 EBPR PAO GAO activated sludge FISH Kinetic and phylogenetic characterization of an anaerobic dechlorinating microbial community ROSSETTI S., BLACKALL L.L., MAJONE M., HUGENHOLTZ P., PLUMB J., TANDOI V. 1,6 Water Research Institute, Via Reno 1, 00198, Rome, Italy; 2,4,5 Department of Microbiology and Parasitology, The University of Queensland, Brisbane, 4072, Australia 3 Department of Chemistry, University of Rome “La Sapienza”, P.le Aldo Moro 5, 00185 Rome, Italy A laboratory scale sequencing batch reactor SBR operating for enhanced biological phosphorus removal EBPR and fed with a mixture of volatile fatty acids VFAs showed stable and efficient EBPR capacity over a four year period. Phosphorus P , poly–b–hydroxyalkanoate PHA and glycogen cycling consistent with classical anaerobic/aerobic EBPR were demonstrated with the order of anaerobic VFA uptake being propionate, acetate then butyrate. The SBR was operated without pH control and 63.67 ± 13.86 mg P/L was released anaerobically. The P% of the sludge fluctuated between 6% and 10% over the operating period average of 8.04 ± 1.31% . Four main morphological types of floc forming bacteria were observed in the sludge during one year of intensive microscopic observation. Two of them were mainly responsible for anaerobic/aerobic P and PHA transformations. Fluorescence in situ hybridisation FISH and post FISH chemical staining for intracellular polyphosphate and PHA were used to determine that “Candidatus Accumulibacter phosphatis” was the most abundant polyphosphate accumulating organism PAO , forming large clusters of coccobacilli 1.0 1.5 µm and comprising 53% of the sludge bacteria. Also by these methods, large coccobacillus shaped gammaproteobacteria 2.5 3.5 µm from a recently described novel cluster were glycogen accumulating organisms GAOs comprising 13% of the bacteria. Tetrad forming organisms TFOs consistent with the “G bacterium” morphotype were alphaproteobacteria, but not Amaricoccus spp., and comprised 25% of all bacteria. According to chemical staining, TFOs were occasionally able to store PHA anaerobically and utilise it aerobically 149 Articolo in rivista simona.rossetti ROSSETTI SIMONA valter.tandoi TANDOI VALTER