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TitleAnaerobic digestion of mixed urban biowaste: The microbial community shift towards stability
AbstractAnaerobic digestion is applied worldwide to treat food waste (FW) with the aim of obtaining renewable bioenergy by exploiting the methane gas produced. However, there are several problems in practical applications, primarily due to system instability. Although exhaustive knowledge regarding anaerobic microbial community composition has been established, few studies have investigated long-term correlations between microbial consortia, operative conditions and feedstock characteristics. Here, microbial community shifts as a response to feedstock variations were investigated in long-term semi-continuous systems, which were evaluated by an in situ cell detection method and 16S rRNA gene amplicon sequencing. FW digestion showed progressive system instability caused by the inhibition of methanogens, which resulted in volatile fatty acid accumulation and process failure at the low organic loading rate (OLR). Conversely, by co-digesting FW with waste-activated sludge (WAS), a stable process with methane yields of up to 0.27 Nm kgVS for OLR = 1.7 gVS Ld was achieved. This stabilizing effect was not related to the buffering capacity of WAS, but to its capacity to avoid volatile fatty acid accumulation and falls in pH by overcoming methanogenic activity inhibition. WAS addition promoted the establishment of a stable and active archaeal population in anaerobic co-digestion (AcoD) reactors. The continuous supply of trace elements together with the seeding of microbial functional groups were the main drivers that positively affected process stability.
SourceNew biotechnology (Print) 55, pp. 108–117
KeywordsAnaerobic co-digestionFood wasteWaste activated sludgeinhibitiontrace elements16S rRNA gene amplicon sequencing
JournalNew biotechnology (Print)
EditorElsevier Life Science, Amsterdam, Paesi Bassi
Year2020
TypeArticolo in rivista
DOI10.1016/j.nbt.2019.10.008
AuthorsTonanzi B.; Braguglia C.M.; Gallipoli A.; Montecchio D.; Pagliaccia P.; Rossetti S.; Gianico A.
Text420311 2020 10.1016/j.nbt.2019.10.008 Scopus 2 s2.0 85074172277 ISI Web of Science WOS WOS 000505030800014 Anaerobic co digestion Food waste Waste activated sludge inhibition trace elements 16S rRNA gene amplicon sequencing Anaerobic digestion of mixed urban biowaste The microbial community shift towards stability Tonanzi B.; Braguglia C.M.; Gallipoli A.; Montecchio D.; Pagliaccia P.; Rossetti S.; Gianico A. Water Research Institute IRSA CNR, Area della Ricerca RM1, Via Salaria km 29.300, Monterotondo Roma , 00015, Water Research Institute IRSA CNR, Area della Ricerca RM1, Via Salaria km 29.300, 00015 Monterotondo Roma , Italy, , Italy Anaerobic digestion is applied worldwide to treat food waste FW with the aim of obtaining renewable bioenergy by exploiting the methane gas produced. However, there are several problems in practical applications, primarily due to system instability. Although exhaustive knowledge regarding anaerobic microbial community composition has been established, few studies have investigated long term correlations between microbial consortia, operative conditions and feedstock characteristics. Here, microbial community shifts as a response to feedstock variations were investigated in long term semi continuous systems, which were evaluated by an in situ cell detection method and 16S rRNA gene amplicon sequencing. FW digestion showed progressive system instability caused by the inhibition of methanogens, which resulted in volatile fatty acid accumulation and process failure at the low organic loading rate OLR . Conversely, by co digesting FW with waste activated sludge WAS , a stable process with methane yields of up to 0.27 Nm kgVS for OLR = 1.7 gVS Ld was achieved. This stabilizing effect was not related to the buffering capacity of WAS, but to its capacity to avoid volatile fatty acid accumulation and falls in pH by overcoming methanogenic activity inhibition. WAS addition promoted the establishment of a stable and active archaeal population in anaerobic co digestion AcoD reactors. The continuous supply of trace elements together with the seeding of microbial functional groups were the main drivers that positively affected process stability. 55 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85074172277 origin=inward 15/10/2019 Pubblicato online il 18/11/2019 Anaerobic digestion of mixed urban biowaste The microbial community shift towards stability Manuscript 2020 Tonanzi et al., NewBiotechnol AD.pdf Articolo in rivista Elsevier Life Science 1871 6784 New biotechnology Print New biotechnology Print New biotechnology. Print PAGLIACCIA PAMELA TONANZI BARBARA simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA andrea.gianico GIANICO ANDREA daniele.montecchio MONTECCHIO DANIELE agata.gallipoli GALLIPOLI AGATA