Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
| Abstract | The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus. |
| Source | Fermentation (Basel) 4 (3) |
| Keywords | Fluorescence in situ Hybridization (FISH)Hardwood spent sulfite liquorMixed microbial culturesPolyhydroxyalkanoatesShort-chain organic acids |
| Journal | Fermentation (Basel) |
| Editor | MDPI AG, Basel, Svizzera |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.3390/fermentation4030058 |
| Authors | Queirós, Diogo; Rangel, Catarina; Lemos, Paulo Costa; Rossetti, Simona; Serafim, Luísa Seuanes |
| Text | 417988 2018 10.3390/fermentation4030058 Scopus 2 s2.0 85062238618 Fluorescence in situ Hybridization FISH Hardwood spent sulfite liquor Mixed microbial cultures Polyhydroxyalkanoates Short chain organic acids Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates producing organisms Queiros, Diogo; Rangel, Catarina; Lemos, Paulo Costa; Rossetti, Simona; Serafim, Luisa Seuanes Istituto di Ricerca sulle Acque, Italy; Universidade de Aveiro; Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa The effectiveness of polyhydroxyalkanoates PHAs production process from a waste stream is determined by the selection of a suitable mixed microbial culture MMC . In this work, a feedstock from the paper industry, hardwood spent sulfite liquor HSSL , supplemented with short chain organic acids SCOAs to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% 3HB 3HV 76 24 . The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified Betaproteobacteria 44.7 ± 2.7% , Alphaproteobacteria 13.6 ± 1.3% and Gammaproteobacteria 2.40 ± 1.1% and Bacteroidetes 9.20 ± 3.8% . Inside the Betaproteobacteria class, Acidovorax 71% was the dominant genus. 4 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85062238618 origin=inward Articolo in rivista MDPI AG 2311 5637 Fermentation Basel Fermentation Basel Fermentation Fermentation simona.rossetti ROSSETTI SIMONA |