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DatoValore
TitleMonitoring, isolation and characterization of Microthrix parvicella strains from a Chinese wastewater treatment plant
AbstractMicrothrix parvicella is a filamentous bacterium that frequently causes severe bulking events in wastewater treatment plants (WWTPs) worldwide. In this study, sludge properties and dynamics of filamentous bacteria in a Beijing WWTP seasonally suffering from M. parvicella bulking were continuously monitored over a duration of 15 months, and the correlations between M. parvicella and operating parameters were evaluated. The predominance of M. parvicella was observed at low temperatures (14-18.8 degrees C) with the relative abundance of around 30% (estimated by both qPCR and FISH analysis). Using micromanipulation technology, 545 filaments of M. parvicella were micromanipulated from bulking sludge (SVI> 180 mL g(-1)) on six different media. After 3-month purification and enrichment, six strains, phylogenetically closely related to Candidatus Microthrix parvicella, were successfully acquired on R(2)A medium (20 degrees C) in pure cultures. Considering the limitation and extremely slow growth rate of M. parvicella filaments, newly isolated strains represent valuable sources for further investigations on the physiology and behavior of this filamentous bacterium, with the focus on the establishment of bulking control strategy.
SourceWater science and technology 79 (7), pp. 1406–1416
Keywordsfilamentous bacteriaisolationmicromanipulationMicrothrix parvicellasludge bulking
JournalWater science and technology
EditorIWA Publishing, London,
Year2019
TypeArticolo in rivista
DOI10.2166/wst.2019.136
AuthorsFan, Niansi; Yang, Min; Rossetti, Simona; Levantesi, Caterina; Qi, Rong
Text417987 2019 10.2166/wst.2019.136 ISI Web of Science WOS 000468740500018 filamentous bacteria isolation micromanipulation Microthrix parvicella sludge bulking Monitoring, isolation and characterization of Microthrix parvicella strains from a Chinese wastewater treatment plant Fan, Niansi; Yang, Min; Rossetti, Simona; Levantesi, Caterina; Qi, Rong Hangzhou Normal Univ; Chinese Acad Sci; CNR Microthrix parvicella is a filamentous bacterium that frequently causes severe bulking events in wastewater treatment plants WWTPs worldwide. In this study, sludge properties and dynamics of filamentous bacteria in a Beijing WWTP seasonally suffering from M. parvicella bulking were continuously monitored over a duration of 15 months, and the correlations between M. parvicella and operating parameters were evaluated. The predominance of M. parvicella was observed at low temperatures 14 18.8 degrees C with the relative abundance of around 30% estimated by both qPCR and FISH analysis . Using micromanipulation technology, 545 filaments of M. parvicella were micromanipulated from bulking sludge SVI> 180 mL g 1 on six different media. After 3 month purification and enrichment, six strains, phylogenetically closely related to Candidatus Microthrix parvicella, were successfully acquired on R 2 A medium 20 degrees C in pure cultures. Considering the limitation and extremely slow growth rate of M. parvicella filaments, newly isolated strains represent valuable sources for further investigations on the physiology and behavior of this filamentous bacterium, with the focus on the establishment of bulking control strategy. 79 Published version Articolo in rivista IWA Publishing 0273 1223 Water science and technology Water science and technology Water science and technology. Water sci. technol. simona.rossetti ROSSETTI SIMONA caterina.levantesi LEVANTESI CATERINA