Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbiome dynamics and phaC synthase genes selected in a pilot plant producing polyhydroxyalkanoate from the organic fraction of urban waste |
| Abstract | This study analyses the bacterial population dynamics of a mixed microbial community (MMC) selected in a pilot plant producing polyhydroxyalkanoate (PHA) from the fermentation of the organic fraction of urban waste (OFMSW) and sewage sludge (SS). 165 rRNA gene high-throughput sequencing revealed the occurrence of a variety of PHA accumulating bacteria that ensured a stable PHA production in an open system operating with real substrates and without temperature control. The Volatile Fatty Acids (VIA) changes in the feed and the temperature variation affected the dynamics of the PHA-accumulating bacteria over the plant operation. Remarkably, the higher PHA content was associated to a MMC largely comprising of Hydrogenophaga species during the operation at higher working temperature. The involvement of a heterogeneous PHA-accumulating MMC was associated with a high phaC synthase genes biodiversity confirming the occurrence of a functional redundancy. (C) 2019 Elsevier B.V. All rights reserved. |
| Source | Science of the total environment 689, pp. 765–773 |
| Keywords | Polyhydroxyalkanoates (PHA)Mixed microbial culture (MMC)Organic Fraction of Municipal Solid Waste (OFMSW)Sewage sludge (SS)16S rRNA gene high-throughput sequencing phaC synthase genes |
| Journal | Science of the total environment |
| Editor | Elsevier, Lausanne ;, Paesi Bassi |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.scitotenv.2019.06.491 |
| Authors | Crognale, Simona; Tonanzi, Barbara; Valentino, Francesco; Majone, Mauro; Rossetti, Simona |
| Text | 417986 2019 10.1016/j.scitotenv.2019.06.491 ISI Web of Science WOS 000482379400074 Scopus 2 s2.0 85068254956 Polyhydroxyalkanoates PHA Mixed microbial culture MMC Organic Fraction of Municipal Solid Waste OFMSW Sewage sludge SS 16S rRNA gene high throughput sequencing phaC synthase genes Microbiome dynamics and phaC synthase genes selected in a pilot plant producing polyhydroxyalkanoate from the organic fraction of urban waste Crognale, Simona; Tonanzi, Barbara; Valentino, Francesco; Majone, Mauro; Rossetti, Simona CNR; Univ Roma La Sapienza This study analyses the bacterial population dynamics of a mixed microbial community MMC selected in a pilot plant producing polyhydroxyalkanoate PHA from the fermentation of the organic fraction of urban waste OFMSW and sewage sludge SS . 165 rRNA gene high throughput sequencing revealed the occurrence of a variety of PHA accumulating bacteria that ensured a stable PHA production in an open system operating with real substrates and without temperature control. The Volatile Fatty Acids VIA changes in the feed and the temperature variation affected the dynamics of the PHA accumulating bacteria over the plant operation. Remarkably, the higher PHA content was associated to a MMC largely comprising of Hydrogenophaga species during the operation at higher working temperature. The involvement of a heterogeneous PHA accumulating MMC was associated with a high phaC synthase genes biodiversity confirming the occurrence of a functional redundancy. C 2019 Elsevier B.V. All rights reserved. 689 Published version Microbiome dynamics and phaC synthase genes selected in a pilot plant producing polyhydroxyalkanoate from the organic fraction of urban waste Science of the Total Environment, 689, 765 773, 2019 Crognale et al STOTEN 2019.pdf Articolo in rivista Elsevier 0048 9697 Science of the total environment Science of the total environment Sci. total environ. simona.rossetti ROSSETTI SIMONA simona.crognale CROGNALE SIMONA RES URBIS REsources from URban BIo waSte |