| Title | Structural characteristics of microbial biofilms in marine environment: colonization of macro-plastics deployed in the Ross Sea (Antarctica) |
| Abstract | To date, microbial biofilm communities in Terra Nova Bay (Ross Sea, Antarctica)are still scarcely explored regarding their structure and function; moreover, no data are currently available regarding possible effects of environmental and/or anthropic forcing on microbial colonization. Funded by the Italian National Programme of Antarctic Research (PNRA16_00105), the project ANT-Biofilm started in November 2017 with the overall objective of studying microbial colonization of benthic environments, focusing on microbial abundance, activity and diversity, and larval settlement in response to natural or anthropogenic disturbances.
Here we present the preliminary results on the structural characteristics and diversity of the microbial communities associated to two artificial substrates deployed in a coastal Antarctic environment of the Ross Sea, close to the Italian Research Station Mario Zucchelli. Polyethylene (PVC) panels and polyethylene (PE) coupons were placed at -5 and -20 m of depth in two sites in Terra Nova Bay (Road Bay and Tethys Bay, differently impacted by anthropic pressure) and collected after 3, 9 and 12 months during the 33 Italian Expedition. Bacterial community composition was studied at single cell level, by Catalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) combined with Confocal Laser Scanning Microscopy (CLSM). Moreover 16s rRNA gene sequencing was applied to compare the diversity of the planktonic and substrate-associated microbial communities. |
| Source | Ocean4biotech meeting, 20/12/2019 |
| Keywords | microbial biofilmplasticAntarcticaCARD-FISH |
| Year | 2019 |
| Type | Contributo in atti di convegno |
| Authors | Fazi S., Scarinci R., Severini M. , Papale M., Lo Giudice A., Azzaro M., Dell'Acqua O., Caruso G. |
| Text | 414382 2019 microbial biofilm plastic Antarctica CARD FISH Structural characteristics of microbial biofilms in marine environment colonization of macro plastics deployed in the Ross Sea Antarctica Fazi S., Scarinci R., Severini M. , Papale M., Lo Giudice A., Azzaro M., Dell Acqua O., Caruso G. 1 IRSA CNR, Water Research Institute, Italian National Research Council CNR , Via Salaria km 29.300 CP10 00015 Monterotondo Rome 2 ISP CNR, Institute of Polar Sciences, CNR, Spianata S. Raineri 86 98122 Messina 3 DISTAV, Department of Earth, Environmental and Life Sciences, University of Genoa, Corso Europa, 26, 16132 Genoa To date, microbial biofilm communities in Terra Nova Bay Ross Sea, Antarctica are still scarcely explored regarding their structure and function; moreover, no data are currently available regarding possible effects of environmental and/or anthropic forcing on microbial colonization. Funded by the Italian National Programme of Antarctic Research PNRA16_00105 , the project ANT Biofilm started in November 2017 with the overall objective of studying microbial colonization of benthic environments, focusing on microbial abundance, activity and diversity, and larval settlement in response to natural or anthropogenic disturbances. Here we present the preliminary results on the structural characteristics and diversity of the microbial communities associated to two artificial substrates deployed in a coastal Antarctic environment of the Ross Sea, close to the Italian Research Station Mario Zucchelli. Polyethylene PVC panels and polyethylene PE coupons were placed at 5 and 20 m of depth in two sites in Terra Nova Bay Road Bay and Tethys Bay, differently impacted by anthropic pressure and collected after 3, 9 and 12 months during the 33 Italian Expedition. Bacterial community composition was studied at single cell level, by Catalysed Reporter Deposition Fluorescence In Situ Hybridization CARD FISH combined with Confocal Laser Scanning Microscopy CLSM . Moreover 16s rRNA gene sequencing was applied to compare the diversity of the planktonic and substrate associated microbial communities. Preprint Ocean4biotech meeting 20/12/2019 Internazionale Contributo Contributo in atti di convegno maria.papale PAPALE MARIA gabriella.caruso CARUSO GABRIELLA maurizio.azzaro AZZARO MAURIZIO stefano.fazi FAZI STEFANO angelina.logiudice LO GIUDICE ANGELINA DTA.AD002.343.001 Colonizzazione microbica di ambienti bentonici in Antartide risposte di abbondanze, diversita e attivita microbiche ed insediamento larvale a disturbi naturali o antropici e ricerca di metaboliti secondari Programma Nazionale di Ricerca in Antarti |
