Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbial functional responses to water quality alterations in a landfill-impacted aquifer |
| Abstract | Aquifers represent the largest accessible and fragile resource of freshwater on Earth. The municipal solid waste disposal in landfills, followed by the dispersion of leachate, is among the major threats for groundwater ecosystems, which harbour different microbial taxa generally adapted to low availability of organic carbon and nutrients. The aim of this study was to explore how groundwater quality can influence major microbial community characteristics, and particularly the functional diversity and metabolic responsiveness to a spectrum of organic carbon inputs. We hypothesized that the landfill-induced water quality changes will represent relevant stress factor for the ecological dynamics of groundwater microbial communities. A comprehensive study of a deep aquifer underlying a municipal solid waste landfill was performed in Central Italy across a network of twelve piezometers. Along with major physical-chemical parameters, the microbial community structure and the microbiological contamination (total coliforms and Escherichia coli), were assessed respectively by flow cytometry and the test Colilert-18. The BIOLOG EcoPlates(TM) assay was used to analyze the metabolic potential and the organic substrate utilization profiles. Three groundwater groups with distinct hydrogeochemical characteristics (pristine, mixed and altered conditions) were identified across the landfill area. Relatively higher total cell counts and the presence of E. coli under altered conditions suggested the occurrence of land-derived inputs. The kinetic analysis of the BIOLOG profiles showed that the aquatic microorganisms in samples from altered conditions were relatively more efficient in metabolizing a larger number of organic substrates, including complex structural molecules of xenobiotic origin. Our outcomes showed that the landfill-induced groundwater quality changes can directly affect the oxidation-reduction potential and the overall carbon cycle. Moreover, a multidisciplinary methodological approach is likely to represent a supportive tool to better characterize the impact of groundwater quality alterations, with potential consequences on the ecosystem functioning. |
| Source | SAME - 16th Symposium of Aquatic Microbial Ecology, POTSDAM, GERMANY, 1st - 6th SEPTEMBER 2019 |
| Keywords | Biolog Ecoplatesflow cytometrymicrobial communitymetabolic fingerprintgroundwater qualityhydrogeochemistry |
| Year | 2019 |
| Type | Poster |
| Authors | Melita M, Amalfitano S, Preziosi E, Ghergo S, Frollini E, Parrone D, Zoppini A |
| Text | 413033 2019 Biolog Ecoplates flow cytometry microbial community metabolic fingerprint groundwater quality hydrogeochemistry Microbial functional responses to water quality alterations in a landfill impacted aquifer Melita M, Amalfitano S, Preziosi E, Ghergo S, Frollini E, Parrone D, Zoppini A IRSA CNR Aquifers represent the largest accessible and fragile resource of freshwater on Earth. The municipal solid waste disposal in landfills, followed by the dispersion of leachate, is among the major threats for groundwater ecosystems, which harbour different microbial taxa generally adapted to low availability of organic carbon and nutrients. The aim of this study was to explore how groundwater quality can influence major microbial community characteristics, and particularly the functional diversity and metabolic responsiveness to a spectrum of organic carbon inputs. We hypothesized that the landfill induced water quality changes will represent relevant stress factor for the ecological dynamics of groundwater microbial communities. A comprehensive study of a deep aquifer underlying a municipal solid waste landfill was performed in Central Italy across a network of twelve piezometers. Along with major physical chemical parameters, the microbial community structure and the microbiological contamination total coliforms and Escherichia coli , were assessed respectively by flow cytometry and the test Colilert 18. The BIOLOG EcoPlates TM assay was used to analyze the metabolic potential and the organic substrate utilization profiles. Three groundwater groups with distinct hydrogeochemical characteristics pristine, mixed and altered conditions were identified across the landfill area. Relatively higher total cell counts and the presence of E. coli under altered conditions suggested the occurrence of land derived inputs. The kinetic analysis of the BIOLOG profiles showed that the aquatic microorganisms in samples from altered conditions were relatively more efficient in metabolizing a larger number of organic substrates, including complex structural molecules of xenobiotic origin. Our outcomes showed that the landfill induced groundwater quality changes can directly affect the oxidation reduction potential and the overall carbon cycle. Moreover, a multidisciplinary methodological approach is likely to represent a supportive tool to better characterize the impact of groundwater quality alterations, with potential consequences on the ecosystem functioning. Published version SAME 16th Symposium of Aquatic Microbial Ecology POTSDAM, GERMANY 1st 6th SEPTEMBER 2019 Internazionale Contributo Microbial functional responses to water quality alterations in a landfill impacted aquifer copia poster SAME SAME2019_Melita_poster.pdf Poster danieleparrone PARRONE DANIELE eleonorafrollini FROLLINI ELEONORA marcomelita MELITA MARCO stefano.ghergo GHERGO STEFANO elisabetta.preziosi PREZIOSI ELISABETTA stefano.amalfitano AMALFITANO STEFANO annamaria.zoppini ZOPPINI ANNAMARIA DTA.AD002.479.001 Gestione sostenibile ed efficiente delle risorse naturali, degli ecosistemi e della biodiversita |