Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Physiological Profiling and Functional Diversity of Groundwater Microbial Communities in a Municipal Solid Waste Landfill Area |
| Abstract | The disposal of municipal solid wastes in landfills represents a major threat for aquifer environments at the global scale. The aim of this study was to explore how groundwater geochemical characteristics can influence the microbial community functioning and the potential degradation patterns of selected organic substrates in response to different levels of landfill-induced alterations. Groundwaters collected from a landfill area were monitored by assessing major physical-chemical parameters and the microbiological contamination levels (total coliforms and fecal indicators--Colilert-18). The aquatic microbial community was further characterized by flow cytometry and Biolog EcoPlatesTM assay. Three groundwater conditions (i.e., pristine, mixed, and altered) were identified according to their distinct geochemical profiles. The altered groundwaters showed relatively higher values of organic matter concentration and total cell counts, along with the presence of fecal indicator bacteria, in comparison to samples from pristine and mixed conditions. The kinetic profiles of the Biolog substrate degradation showed that the microbial community thriving in altered conditions was relatively more efficient in metabolizing a larger number of organic substrates, including those with complex molecular structures. We concluded that the assessment of physiological profiling and functional diversity at the microbial community level could represent a supportive tool to understand the potential consequences of the organic contamination of impacted aquifers, thus complementing the current strategies for groundwater management. |
| Source | Water (Basel) 11 (2624), pp. 2624 |
| Keywords | Biolog EcoPlatesflow cytometrymicrobial communitymetabolic fingerprintgroundwater qualityhydrogeochemistry |
| Journal | Water (Basel) |
| Editor | Molecular Diversity Preservation International, Basel, |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.3390/w11122624 |
| Authors | Marco Melita , Stefano Amalfitano , Elisabetta Preziosi , Stefano Ghergo , Eleonora Frollini , Daniele Parrone, Annamaria Zoppini |
| Text | 413020 2019 10.3390/w11122624 ISI Web of Science WOS WOS 000507378600196 Scopus 2 s2.0 85076672384 Biolog EcoPlates flow cytometry microbial community metabolic fingerprint groundwater quality hydrogeochemistry Physiological Profiling and Functional Diversity of Groundwater Microbial Communities in a Municipal Solid Waste Landfill Area Marco Melita , Stefano Amalfitano , Elisabetta Preziosi , Stefano Ghergo , Eleonora Frollini , Daniele Parrone, Annamaria Zoppini Water Research Institute, National Research Council IRSA CNR , 00015 Monterotondo, Rome, Italy The disposal of municipal solid wastes in landfills represents a major threat for aquifer environments at the global scale. The aim of this study was to explore how groundwater geochemical characteristics can influence the microbial community functioning and the potential degradation patterns of selected organic substrates in response to different levels of landfill induced alterations. Groundwaters collected from a landfill area were monitored by assessing major physical chemical parameters and the microbiological contamination levels total coliforms and fecal indicators Colilert 18 . The aquatic microbial community was further characterized by flow cytometry and Biolog EcoPlatesTM assay. Three groundwater conditions i.e., pristine, mixed, and altered were identified according to their distinct geochemical profiles. The altered groundwaters showed relatively higher values of organic matter concentration and total cell counts, along with the presence of fecal indicator bacteria, in comparison to samples from pristine and mixed conditions. The kinetic profiles of the Biolog substrate degradation showed that the microbial community thriving in altered conditions was relatively more efficient in metabolizing a larger number of organic substrates, including those with complex molecular structures. We concluded that the assessment of physiological profiling and functional diversity at the microbial community level could represent a supportive tool to understand the potential consequences of the organic contamination of impacted aquifers, thus complementing the current strategies for groundwater management. 11 Published version https //www.mdpi.com/2073 4441/11/12/2624 07/12/2019 This work was financially supported by the Regione Lazio Area Ciclo Integrato Rifiuti , Italy Det. G9473 of 30/7/2015 and MAD s.r.l., Italy contract 1471/2018 . Physiological Profiling and Functional Diversity of Groundwater Microbial Communities in a Municipal Solid Waste Landfill Area reprint melita et al.water2019.pdf Articolo in rivista Molecular Diversity Preservation International 2073 4441 Water Basel Water Basel Water Basel Water. Basel danieleparrone PARRONE DANIELE eleonorafrollini FROLLINI ELEONORA marcomelita MELITA MARCO stefano.ghergo GHERGO STEFANO elisabetta.preziosi PREZIOSI ELISABETTA stefano.amalfitano AMALFITANO STEFANO annamaria.zoppini ZOPPINI ANNAMARIA TA.P04.032.002 Ruolo delle comunita microbiche nei cicli biogeochimici e nella degradazione degli inquinanti DTA.AD002.026.001 GESTIONE SOSTENIBILE DEGLI ECOSISTEMI ACQUATICI E RISPOSTA AGLI IMPATTI DI ORIGINE NATURALE ED ANTROPICA MONTEROTONDO BRUGHERIO BARI |