Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | First Insight on the Mucus of the Annelid Myxicola infundibulum (Polychaeta, Sabellidae) as a Potential Prospect for Drug Discovery |
| Abstract | Many marine organisms, including invertebrates, produce mucosal matrices having different functions. Besides mechanical protection, the mucus of many invertebrates contains specific compounds to make the animal poisonous and/or distasteful or irritating. The presence of antibiotic molecules is more advantageous for some invertebrates to contrast bacterial attack. In the present study we investigated the mucus of the Mediterranean annelid species Myxicola infundibulum living in a gelatinous envelope made up of dense mucus. Antimicrobial lysozyme-like and antioxidant activities were investigated to highlight the potential interest of the worm mucus as a source of bioactive compounds for biotechnological applications. In order to understand which kind of compounds could be responsible for the detected activities, the mucus of M. infundibulum was chemically characterized in terms of elemental composition, protein, lipid and carbohydrate content. Further chemical characterization was achieved by the advanced analytical technique of multinuclear and multidimensional NMR spectroscopy. NMR spectroscopy revealed the scarcity of lipids which preferentially resulted of alcoholic origin, or otherwise hydroxylate and several aminoacids (valine, leucine and alanine) in the aqueous extract in relation to the protein nature of M. infundibulum mucus. The mucus indeed is mainly composed by water (94% +/- 0.7%) whereas its dry weight is made of proteins (36% +/- 2.3%) followed by lipids (2.9% +/- 0.07%) and carbohydrates (2% +/- 0.31%). The mucus exerted a natural antibacterial lysozyme-like activity corresponding to 1.14 mg mL(-1) of hen egg-white lysozyme and an antioxidant activity corresponding to 483.00 +/- 79.22 nmolTE (Trolox equivalent)/mL sample as Trolox Equivalent Antioxidant Capacity (TEAC) and 276.26 +/- 50.76 nmolTE/mL sample as Oxygen Radical Absorbance Capacity (ORAC). Therefore, our findings have potential implications due to the ongoing explosion of antibiotic resistant infections and the need to discover antibacterial agents. Additionally, the observed antioxidant activity is intriguing taking into account the need to find natural antioxidants useful for human health. |
| Source | Marine drugs 17 (7), pp. 396 |
| Keywords | mucusPolychaetaNMR spectroscopyantioxidant activityantibacterial activity |
| Journal | Marine drugs |
| Editor | MDPI,, Basel, |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.3390/md17070396 |
| Authors | Stabili, Loredana; Licciano, Margherita; Giangrande, Adriana; Gerardi, Carmela; De Pascali, Sandra Angelica; Fanizzi, Francesco Paolo |
| Text | 409842 2019 10.3390/md17070396 ISI Web of Science WOS 000478650600021 mucus Polychaeta NMR spectroscopy antioxidant activity antibacterial activity First Insight on the Mucus of the Annelid Myxicola infundibulum Polychaeta, Sabellidae as a Potential Prospect for Drug Discovery Stabili, Loredana; Licciano, Margherita; Giangrande, Adriana; Gerardi, Carmela; De Pascali, Sandra Angelica; Fanizzi, Francesco Paolo Univ Salento;CNR ISPA UOS Lecce, CNR Istituto di Ricerca sulle Acque Taranto Many marine organisms, including invertebrates, produce mucosal matrices having different functions. Besides mechanical protection, the mucus of many invertebrates contains specific compounds to make the animal poisonous and/or distasteful or irritating. The presence of antibiotic molecules is more advantageous for some invertebrates to contrast bacterial attack. In the present study we investigated the mucus of the Mediterranean annelid species Myxicola infundibulum living in a gelatinous envelope made up of dense mucus. Antimicrobial lysozyme like and antioxidant activities were investigated to highlight the potential interest of the worm mucus as a source of bioactive compounds for biotechnological applications. In order to understand which kind of compounds could be responsible for the detected activities, the mucus of M. infundibulum was chemically characterized in terms of elemental composition, protein, lipid and carbohydrate content. Further chemical characterization was achieved by the advanced analytical technique of multinuclear and multidimensional NMR spectroscopy. NMR spectroscopy revealed the scarcity of lipids which preferentially resulted of alcoholic origin, or otherwise hydroxylate and several aminoacids valine, leucine and alanine in the aqueous extract in relation to the protein nature of M. infundibulum mucus. The mucus indeed is mainly composed by water 94% / 0.7% whereas its dry weight is made of proteins 36% / 2.3% followed by lipids 2.9% / 0.07% and carbohydrates 2% / 0.31% . The mucus exerted a natural antibacterial lysozyme like activity corresponding to 1.14 mg mL 1 of hen egg white lysozyme and an antioxidant activity corresponding to 483.00 / 79.22 nmolTE Trolox equivalent /mL sample as Trolox Equivalent Antioxidant Capacity TEAC and 276.26 / 50.76 nmolTE/mL sample as Oxygen Radical Absorbance Capacity ORAC . Therefore, our findings have potential implications due to the ongoing explosion of antibiotic resistant infections and the need to discover antibacterial agents. Additionally, the observed antioxidant activity is intriguing taking into account the need to find natural antioxidants useful for human health. 17 Published version First Insight on the Mucus of the Annelid Myxicola infundibulum Polychaeta, Sabellidae as a Potential Prospect for Drug Discovery pdf articolo marinedrugs2019.pdf Articolo in rivista MDPI, 1660 3397 Marine drugs Marine drugs Mar. drugs Marine drugs. loredana.stabili STABILI LOREDANA carmela.gerardi GERARDI CARMELA AG.P05.007.001 Biotecnologie per la qualita e sicurezza degli alimenti |