Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | A simple method for rapid purification of phycobiliproteins from Arthrospira platensis and Porphyridium cruentum biomass |
| Abstract | Membrane chromatography was exploited to purify allophycocyanin, phycocyanin and B-phycoerythrin from aqueous extracts of fresh or freeze-dried biomass of Arthrospira platensis (Cyanobacteria) and Porphyridium cruentum (Rhodophyta). The method can be tuned to obtain products having various degree of purity and avoids expensive and time consuming column chromatography and ultrafiltration steps. A commercial polyvinylidene fluoride (PVDF) microfiltration membrane (hydrophilic and low protein binding) was used as an ammonium sulphate responsive stationary phase to carry out the purification process. Analytical grade purity was achieved for phycocyanin (purity = 4.2-4.5, yield = 75-82 %) and B-phycoerythrin (purity = 4.5-4.8, yield = 69-71 %). Good purification, even if lower than analytical grade, was achieved for allophycocyanin (purity = 3.3-3.7, yield = 42-47%). |
| Source | Algal Research 44, pp. Article number 101685 |
| Keywords | Phycobiliprotein purificationMembrane chromatographyHydrophilic microfiltration polyvinylidene fluoride (PVDF) membraneAmmonium sulphateArthrospiraPorphyridium |
| Journal | Algal Research |
| Editor | Elsevier, Netherlands, |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.algal.2019.101685 |
| Authors | Lauceri R., Chini Zittelli G., Torzillo G. |
| Text | 408739 2019 10.1016/j.algal.2019.101685 Scopus 2 s2.0 85074213713 ISI Web of Science WOS 000498792900009 Phycobiliprotein purification Membrane chromatography Hydrophilic microfiltration polyvinylidene fluoride PVDF membrane Ammonium sulphate Arthrospira Porphyridium A simple method for rapid purification of phycobiliproteins from Arthrospira platensis and Porphyridium cruentum biomass Lauceri R., Chini Zittelli G., Torzillo G. Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Largo Tonolli 50, Verbania, Pallanza, VB, 28922, Italy; Consiglio Nazionale delle Ricerche, Istituto per la Bioeconomia, Via Madonna del Piano 10, Sesto, Fiorentino, FI, 50019, Italy Membrane chromatography was exploited to purify allophycocyanin, phycocyanin and B phycoerythrin from aqueous extracts of fresh or freeze dried biomass of Arthrospira platensis Cyanobacteria and Porphyridium cruentum Rhodophyta . The method can be tuned to obtain products having various degree of purity and avoids expensive and time consuming column chromatography and ultrafiltration steps. A commercial polyvinylidene fluoride PVDF microfiltration membrane hydrophilic and low protein binding was used as an ammonium sulphate responsive stationary phase to carry out the purification process. Analytical grade purity was achieved for phycocyanin purity = 4.2 4.5, yield = 75 82 % and B phycoerythrin purity = 4.5 4.8, yield = 69 71 % . Good purification, even if lower than analytical grade, was achieved for allophycocyanin purity = 3.3 3.7, yield = 42 47% . 44 Published version https //doi.org/10.1016/j.algal.2019.101685 26/09/2019 A simple method for rapid purification of phycobiliproteins from Arthrospira platensis and Porphyridium cruentum biomass A_simple_method_for_rapid_puri_cation_of_phycobiliproteins_from_Arthrospira_platensis_and_Porphyridium_cruentum_biomass_2019.pdf Articolo in rivista Elsevier 2211 9264 Algal Research Algal Research rosaria.lauceri LAUCERI ROSARIA graziella.chinizittelli CHINI ZITTELLI GRAZIELLA giuseppe.torzillo TORZILLO GIUSEPPE |