Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Non-endogenous ketocarotenoid accumulation in engineered Synechocystis sp. PCC 6803 |
| Abstract | The cyanobacterium Synechocystis sp. PCC 6803 is a model species commonly employed for biotechnological applications. It is naturally able to accumulate zeaxanthin (Zea) and echinenone (Ech), but not astaxanthin (Asx), which is the highest value carotenoid produced by microalgae, with a wide range of applications in pharmaceutical, cosmetics, food and feed industries. With the aim of finding an alternative and sustainable biological source for the production of Asx and other valuable hydroxylated and ketolated intermediates, the carotenoid biosynthetic pathway of Synechocystis sp. PCC 6803 has been engineered by introducing the 4,4 -carotene oxygenase (CrtW) and 3,3 -carotene hydroxylase (CrtZ) genes from Brevundimonas sp. SD-212 under the control of a temperature-inducible promoter. The expression of exogenous CrtZ led to an increased accumulation of Zea at the expense of Ech, while the expression of exogenous CrtW promoted the production of non-endogenous canthaxanthin and an increase in the Ech content with a concomitant strong reduction of -carotene (-car). When both Brevundimonas sp. SD-212 genes were coexpressed, significant amounts of non-endogenous Asx were obtained accompanied by a strong decrease in -car content. Asx accumulation was higher (approximately 50% of total carotenoids) when CrtZ was cloned upstream of CrtW, but still significant (approximately 30%) when the position of genes was inverted. Therefore, the engineered strains constitute a useful tool for investigating the ketocarotenoid biosynthetic pathway in cyanobacteria and an excellent starting point for further optimisation and industrial exploitation of these organisms for the production of added-value compounds. |
| Source | Physiologia plantarum (Kbh., 1948) 166 (1), pp. 403–412 |
| Keywords | Synechocystis sp. PCC 6803carotenoidhplcastaxanthin |
| Journal | Physiologia plantarum (Kbh., 1948) |
| Editor | Blackwell, Oxford, Regno Unito |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1111/ppl.12900 |
| Authors | Menin Barbara; Santabarbara Stefano; Lami Andrea; Musazzi Simona; Monteleone Francesca Villafiorita; Casazza Anna Paola |
| Text | 407829 2019 10.1111/ppl.12900 ISI Web of Science WOS 000466108300032 Scopus 2 s2.0 85060346472 Synechocystis sp. PCC 6803 carotenoid hplc astaxanthin Non endogenous ketocarotenoid accumulation in engineered Synechocystis sp. PCC 6803 Menin Barbara; Santabarbara Stefano; Lami Andrea; Musazzi Simona; Monteleone Francesca Villafiorita; Casazza Anna Paola IBBA CNR; IBF CNR; IRSA CNR The cyanobacterium Synechocystis sp. PCC 6803 is a model species commonly employed for biotechnological applications. It is naturally able to accumulate zeaxanthin Zea and echinenone Ech , but not astaxanthin Asx , which is the highest value carotenoid produced by microalgae, with a wide range of applications in pharmaceutical, cosmetics, food and feed industries. With the aim of finding an alternative and sustainable biological source for the production of Asx and other valuable hydroxylated and ketolated intermediates, the carotenoid biosynthetic pathway of Synechocystis sp. PCC 6803 has been engineered by introducing the 4,4 carotene oxygenase CrtW and 3,3 carotene hydroxylase CrtZ genes from Brevundimonas sp. SD 212 under the control of a temperature inducible promoter. The expression of exogenous CrtZ led to an increased accumulation of Zea at the expense of Ech, while the expression of exogenous CrtW promoted the production of non endogenous canthaxanthin and an increase in the Ech content with a concomitant strong reduction of carotene car . When both Brevundimonas sp. SD 212 genes were coexpressed, significant amounts of non endogenous Asx were obtained accompanied by a strong decrease in car content. Asx accumulation was higher approximately 50% of total carotenoids when CrtZ was cloned upstream of CrtW, but still significant approximately 30% when the position of genes was inverted. Therefore, the engineered strains constitute a useful tool for investigating the ketocarotenoid biosynthetic pathway in cyanobacteria and an excellent starting point for further optimisation and industrial exploitation of these organisms for the production of added value compounds. 166 Published version https //onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12900 Non endogenous ketocarotenoid accumulation in engineered Synechocystis sp. PCC 6803 pdf Physiologia Plantarum 2019 Menin.pdf Articolo in rivista Blackwell 0031 9317 Physiologia plantarum Kbh., 1948 Physiologia plantarum Kbh., 1948 Physiol. plant. Kbh., 1948 Physiologia plantarum. Kbh., 1948 annapaola.casazza CASAZZA ANNA PAOLA barbara.menin MENIN BARBARA francesca.villafioritamonteleone VILLAFIORITA MONTELEONE FRANCESCA stefano.santabarbara SANTABARBARA STEFANO andrea.lami LAMI ANDREA simona.musazzi MUSAZZI SIMONA DFM.AD004.155.004 CYAnobacteria platform Optimised for bioproduction CYAO IRSA |