Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Carbamazepine is degraded by the bacterial strain Labrys portucalensis F11 |
| Abstract | The occurrence of pharmaceuticals in the environment is a topic of concern. Carbamazepine (CBZ) is a widespread antiepileptic drug and due to its physical-chemical characteristics minimal removal is achieved in conventional water treatments, and thus has been suggested as a molecular marker of wastewater contamination in surface water and groundwater. The present study reports the biotransformation of CBZ by the bacterial strain Labrys portucalensis F11. When supplied as a sole carbon source, a 95.4% biotransformation of 42.69 ?M CBZ was achieved in 30 days. In co-metabolism with acetate, complete biotransformation was attained at a faster rate. Following a target approach, the detection and identification of 14 intermediary metabolites was achieved through UPLC-QTOF/MS/MS. Biotransformation of CBZ by the bacterial strain is mostly based on oxidation, loss of -CHNO group and ketone formation reactions; a biotransformation pathway with two routes is proposed. The toxicity of untreated and treated CBZ solutions was assessed using Vibrio Fischeri and Lepidium sativum acute toxicity tests and Toxi-Chromo Test. The presence of CBZ and/or its degradations products in solution resulted in moderate toxic effect on Vibrio Fischeri, whereas the other organisms were not affected. To the best of our knowledge this is the first report that proposes the metabolic degradation pathway of CBZ by a single bacterial strain. |
| Source | Science of the total environment 690, pp. 739–747 |
| Keywords | BiotransformationCarbamazepineCo-metabolismLabrys portucalensis F11MetabolitesToxicity |
| Journal | Science of the total environment |
| Editor | Elsevier, Lausanne ;, Paesi Bassi |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.scitotenv.2019.06.461 |
| Authors | Bessa, Vânia S.; Moreira, Irina S.; Murgolo, Sapia; Mascolo, Giuseppe; Castro, Paula M.L. |
| Text | 405419 2019 10.1016/j.scitotenv.2019.06.461 Scopus 2 s2.0 85068582180 Biotransformation Carbamazepine Co metabolism Labrys portucalensis F11 Metabolites Toxicity Carbamazepine is degraded by the bacterial strain Labrys portucalensis F11 Bessa, Vania S.; Moreira, Irina S.; Murgolo, Sapia; Mascolo, Giuseppe; Castro, Paula M.L. Istituto di Ricerca Sulle Acque, Bari; Universidade Catolica Portuguesa, Porto The occurrence of pharmaceuticals in the environment is a topic of concern. Carbamazepine CBZ is a widespread antiepileptic drug and due to its physical chemical characteristics minimal removal is achieved in conventional water treatments, and thus has been suggested as a molecular marker of wastewater contamination in surface water and groundwater. The present study reports the biotransformation of CBZ by the bacterial strain Labrys portucalensis F11. When supplied as a sole carbon source, a 95.4% biotransformation of 42.69 M CBZ was achieved in 30 days. In co metabolism with acetate, complete biotransformation was attained at a faster rate. Following a target approach, the detection and identification of 14 intermediary metabolites was achieved through UPLC QTOF/MS/MS. Biotransformation of CBZ by the bacterial strain is mostly based on oxidation, loss of CHNO group and ketone formation reactions; a biotransformation pathway with two routes is proposed. The toxicity of untreated and treated CBZ solutions was assessed using Vibrio Fischeri and Lepidium sativum acute toxicity tests and Toxi Chromo Test. The presence of CBZ and/or its degradations products in solution resulted in moderate toxic effect on Vibrio Fischeri, whereas the other organisms were not affected. To the best of our knowledge this is the first report that proposes the metabolic degradation pathway of CBZ by a single bacterial strain. 690 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85068582180 origin=inward paper paper con Universita Catolica di Porto 2019 STOTEN con Vania .pdf Articolo in rivista Elsevier 0048 9697 Science of the total environment Science of the total environment Sci. total environ. sapia.murgolo MURGOLO SAPIA giuseppe.mascolo MASCOLO GIUSEPPE |