Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Functional Response of MBR Microbial Consortia to Substrate Stress as Revealed by Metaproteomics |
| Abstract | Bacterial consortia have a primary role in the biological degradations occurring in activated sludge for wastewater treatment, for their capacities to metabolize the polluting matter. Therefore, the knowledge of the main metabolic pathways for the degradation of pollutants becomes critical for a correct design and operation of wastewater treatment plants. The metabolic activity of the different bacterial groups in activated sludge is commonly investigated through respirometry. Furthermore, in the last years, the development of "omic" approaches has offered more opportunities to integrate or substitute the conventional microbiological assays and to deeply understand the taxonomy and dynamics of complex microbial consortia. In the present work, an experimental membrane bioreactor (MBR) was set up and operated for the treatment of municipal wastewater, and the effects of a sudden decrease of the organic supply on the activated sludge were investigated. Both respirometric and metaproteomic approaches revealed a resistance of autotrophic bacteria to the substrate stress, and particularly of nitrifying bacteria. Furthermore, metaproteomics allowed the identification of the taxonomy of the microbial consortium based on its protein expression, unveiling the prevalence of Sorangium and Nitrosomonas genera both before and after the organic load decrease. Moreover, it confirmed the results obtained through respirometry and revealed a general expression of proteins involved in metabolism and transport of nitrogen, or belonging to nitrifying species like Nitrosomonas europeae, Nitrosomonas sp. AL212, or Nitrospira defluvii. |
| Source | Microbial ecology |
| Keywords | MBRMetaproteomicsRespirometryProteinsNitrifiers |
| Journal | Microbial ecology |
| Editor | Springer-Verlag,, New York,, Stati Uniti d'America |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00248-019-01360-4 |
| Authors | Carlo Salerno, Giovanni Berardi, Giuseppe Laera, Alfieri Pollice |
| Text | 402130 2019 10.1007/s00248 019 01360 4 Scopus 2 s2.0 85071622912 ISI Web of Science WOS WOS 000495294900008 MBR Metaproteomics Respirometry Proteins Nitrifiers Functional Response of MBR Microbial Consortia to Substrate Stress as Revealed by Metaproteomics Carlo Salerno, Giovanni Berardi, Giuseppe Laera, Alfieri Pollice IRSA CNR Bacterial consortia have a primary role in the biological degradations occurring in activated sludge for wastewater treatment, for their capacities to metabolize the polluting matter. Therefore, the knowledge of the main metabolic pathways for the degradation of pollutants becomes critical for a correct design and operation of wastewater treatment plants. The metabolic activity of the different bacterial groups in activated sludge is commonly investigated through respirometry. Furthermore, in the last years, the development of omic approaches has offered more opportunities to integrate or substitute the conventional microbiological assays and to deeply understand the taxonomy and dynamics of complex microbial consortia. In the present work, an experimental membrane bioreactor MBR was set up and operated for the treatment of municipal wastewater, and the effects of a sudden decrease of the organic supply on the activated sludge were investigated. Both respirometric and metaproteomic approaches revealed a resistance of autotrophic bacteria to the substrate stress, and particularly of nitrifying bacteria. Furthermore, metaproteomics allowed the identification of the taxonomy of the microbial consortium based on its protein expression, unveiling the prevalence of Sorangium and Nitrosomonas genera both before and after the organic load decrease. Moreover, it confirmed the results obtained through respirometry and revealed a general expression of proteins involved in metabolism and transport of nitrogen, or belonging to nitrifying species like Nitrosomonas europeae, Nitrosomonas sp. AL212, or Nitrospira defluvii. Published version https //link.springer.com/article/10.1007%2Fs00248 019 01360 4 10/02/2019 Paper in formato PDF 2019 Salerno substrate stress response metaproteomics .pdf Articolo in rivista Springer Verlag, 0095 3628 Microbial ecology Microbial ecology Microb Ecol Microbial ecology. alfieri.pollice POLLICE ALFIERI giuseppe.laera LAERA GIUSEPPE giovanni.berardi BERARDI GIOVANNI carlo.salerno SALERNO CARLO |