| Abstract | The Water Framework Directive (WFD) regulates freshwater and coastal water quality assessment in Europe.
Chemical and ecological water quality status is based on measurements of chemical pollutants in water and biota together with other indicators such as temperature, nutrients, species compositions (phytoplankton, microalgae, benthos and fish) and hydromorphological conditions. However, in the current strategy a link between the chemical and the ecological status is missing. In the present WFD, no microbiological indicators are foreseen for integrating the different anthropogenic pressures, including mixtures of chemicals, nutrients and
temperature changes, to provide a holistic view of the freshwater ecosystem water quality. The main aim of this work was to evaluate if natural microbial populations can be valuable indicators of multiple stressors (e.g. chemical pollutants, temperature, nutrients etc.) to guide preventive and remediation actions by water authorities. A preliminary survey was conducted to identify four sites reflecting a contamination gradient from the source to the mouth of a river suitable to the objectives of the European Marie Curie project, MicroCoKit. The River Tiber (Italy) was selected as a pilot case study to investigate the correlation between bacteria taxa and the chemical status of the river. The main physicochemical parameters, inorganic elements, organic pollutants and naturalmicrobial community compositionwere assessed at four selected sites corresponding to pristine, agricultural, industrial and urban areas for three consecutive years. The overall chemical results indicated a correspondence between different groups of contaminants and the main contamination sources at the selected sampling points. Phylogenetic analysis of the microbial community analyzed by Fluorescence In Situ Hybridization method (FISH) revealed differences among the four sampling sites which could reflect an adaptive bacterial response to the different anthropogenic pressures. |
| Authors | Saccà ML, Ferrero VEV, Loos R, Di Lenola M, Tavazzi S, Grenni P, Ademollo N, Patrolecco L, Huggett J, Barra Caracciolo A, Lettieri T |
| Text | 401919 2019 10.1016/j.scitotenv.2019.04.011 Scopus 2 s2.0 85064086730 ISI Web of Science WoS WOS 000466418300002 Freshwater Anthropogenic pollution Stressors Bioindicators Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river Sacca ML, Ferrero VEV, Loos R, Di Lenola M, Tavazzi S, Grenni P, Ademollo N, Patrolecco L, Huggett J, Barra Caracciolo A, Lettieri T Di Lenola, Grenni, Ademollo, Patrolecco, Barra Caracciolo National Research Council,Water Research Institute, Via Salaria km 29,300, 00015 9 Monterotondo, Rome, Italy b Ferrero Molecular and Cell Biology team, LGC, Queens Road, Teddington, Middlesex TW11 0LY, United Kingdom cLettieri, Loos European Commission, Joint Research Centre JRC , Ispra, Italy d Hugget School of Biosciences Medicine, Faculty of Health Medical Science, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom The Water Framework Directive WFD regulates freshwater and coastal water quality assessment in Europe. Chemical and ecological water quality status is based on measurements of chemical pollutants in water and biota together with other indicators such as temperature, nutrients, species compositions phytoplankton, microalgae, benthos and fish and hydromorphological conditions. However, in the current strategy a link between the chemical and the ecological status is missing. In the present WFD, no microbiological indicators are foreseen for integrating the different anthropogenic pressures, including mixtures of chemicals, nutrients and temperature changes, to provide a holistic view of the freshwater ecosystem water quality. The main aim of this work was to evaluate if natural microbial populations can be valuable indicators of multiple stressors e.g. chemical pollutants, temperature, nutrients etc. to guide preventive and remediation actions by water authorities. A preliminary survey was conducted to identify four sites reflecting a contamination gradient from the source to the mouth of a river suitable to the objectives of the European Marie Curie project, MicroCoKit. The River Tiber Italy was selected as a pilot case study to investigate the correlation between bacteria taxa and the chemical status of the river. The main physicochemical parameters, inorganic elements, organic pollutants and naturalmicrobial community compositionwere assessed at four selected sites corresponding to pristine, agricultural, industrial and urban areas for three consecutive years. The overall chemical results indicated a correspondence between different groups of contaminants and the main contamination sources at the selected sampling points. Phylogenetic analysis of the microbial community analyzed by Fluorescence In Situ Hybridization method FISH revealed differences among the four sampling sites which could reflect an adaptive bacterial response to the different anthropogenic pressures. 673 Published version https //www.scopus.com/record/display.uri eid=2 s2.0 85064086730 origin=resultslist sort=plf f src=s st1= st2= sid=d0816036af23660d29c348f95159211f sot=b sdt=b sl=130 s=TITLE ABS KEY%28Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber River%29 relpos=0 citeCnt=5 searchTerm= 01/04/2019 Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river Paper STOTEN_Tevere2019_pdf.pdf Articolo in rivista Elsevier 0048 9697 Science of the total environment Science of the total environment Sci. total environ. DI LENOLA MARTINA luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA nicoletta.ademollo ADEMOLLO NICOLETTA MICROCOKIT Microbial Community based sequencing analysis linked to anthropogenic pressures MicroCoKit to address the water quality DTA.AD002.010.001 MicroCokit ROMA |
