Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | The Jellyfish Rhizostoma pulmo (Cnidaria): Biochemical Composition of Ovaries and Antibacterial Lysozyme-like Activity of the Oocyte Lysate |
| Abstract | Jellyfish outbreaks in marine coastal areas represent an emergent problem worldwide, with negative consequences on human activities and ecosystem functioning. However, potential positive effects of jellyfish biomass proliferation may be envisaged as a natural source of bioactive compounds of pharmaceutical interest. We investigated the biochemical composition of mature female gonads and lysozyme antibacterial activity of oocytes in the Mediterranean barrel jellyfish Rhizostoma pulmo. Chemical characterization was performed by means of multinuclear and multidimensional NMR spectroscopy. The ovaries of R. pulmo were mainly composed of water (93.7 +/- 1.9% of wet weight), with organic matter (OM) and dry weight made respectively of proteins (761.76 +/- 25.11 mu g mg(-1) and 45.7 +/- 1.5%), lipids (192.17 +/- 10.56 mu g mg(-1) and 9.6 +/- 0.6%), and carbohydrates (59.66 +/- 2.72 mu g mg(-1) and 3.7 +/- 0.3%). The aqueous extract of R. pulmo gonads contained free amino acids, organic acids, and derivatives; the lipid extract was composed of triglycerides (TG), polyunsaturated fatty acids (PUFAs), diunsaturated fatty acids (DUFAs), monounsaturated fatty acids (MUFAs), saturated fatty acids (SFAs), and minor components such as sterols and phospholipids. The R. pulmo oocyte lysate exhibited an antibacterial lysozyme-like activity (mean diameter of lysis of 9.33 +/- 0.32 mm corresponding to 1.21 mg/mL of hen egg-white lysozyme). The occurrence of defense molecules is a crucial mechanism to grant healthy development of mature eggs and fertilized embryos (and the reproductive success of the species) by preventing marine bacterial overgrowth. As a corollary, these results call for future investigations for an exploitation of R. pulmo biomasses as a resource of bioactive metabolites of biotechnological importance including pharmaceuticals and nutrition. |
| Source | Marine drugs 17 (1) |
| Keywords | antibacterial activityNMR spectroscopybiochemical characterizationjellyfish blooms |
| Journal | Marine drugs |
| Editor | MDPI,, Basel, |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.3390/md17010017 |
| Authors | Stabili, Loredana; Rizzo, Lucia; Fanizzi, Francesco Paolo; Angile, Federica; Del Coco, Laura; Girelli, Chiara Roberta; Lomartire, Silvia; Piraino, Stefano; Basso, Lorena |
| Text | 400032 2019 10.3390/md17010017 ISI Web of Science WOS 000458053200017 antibacterial activity NMR spectroscopy biochemical characterization jellyfish blooms The Jellyfish Rhizostoma pulmo Cnidaria Biochemical Composition of Ovaries and Antibacterial Lysozyme like Activity of the Oocyte Lysate Stabili, Loredana; Rizzo, Lucia; Fanizzi, Francesco Paolo; Angile, Federica; Del Coco, Laura; Girelli, Chiara Roberta; Lomartire, Silvia; Piraino, Stefano; Basso, Lorena Univ Salento; CNR; CoNISMa; Stn Zool Anton Dohrn Jellyfish outbreaks in marine coastal areas represent an emergent problem worldwide, with negative consequences on human activities and ecosystem functioning. However, potential positive effects of jellyfish biomass proliferation may be envisaged as a natural source of bioactive compounds of pharmaceutical interest. We investigated the biochemical composition of mature female gonads and lysozyme antibacterial activity of oocytes in the Mediterranean barrel jellyfish Rhizostoma pulmo. Chemical characterization was performed by means of multinuclear and multidimensional NMR spectroscopy. The ovaries of R. pulmo were mainly composed of water 93.7 / 1.9% of wet weight , with organic matter OM and dry weight made respectively of proteins 761.76 / 25.11 mu g mg 1 and 45.7 / 1.5% , lipids 192.17 / 10.56 mu g mg 1 and 9.6 / 0.6% , and carbohydrates 59.66 / 2.72 mu g mg 1 and 3.7 / 0.3% . The aqueous extract of R. pulmo gonads contained free amino acids, organic acids, and derivatives; the lipid extract was composed of triglycerides TG , polyunsaturated fatty acids PUFAs , diunsaturated fatty acids DUFAs , monounsaturated fatty acids MUFAs , saturated fatty acids SFAs , and minor components such as sterols and phospholipids. The R. pulmo oocyte lysate exhibited an antibacterial lysozyme like activity mean diameter of lysis of 9.33 / 0.32 mm corresponding to 1.21 mg/mL of hen egg white lysozyme . The occurrence of defense molecules is a crucial mechanism to grant healthy development of mature eggs and fertilized embryos and the reproductive success of the species by preventing marine bacterial overgrowth. As a corollary, these results call for future investigations for an exploitation of R. pulmo biomasses as a resource of bioactive metabolites of biotechnological importance including pharmaceuticals and nutrition. 17 Published version Articolo in rivista MDPI, 1660 3397 Marine drugs Marine drugs Mar. drugs Marine drugs. loredana.stabili STABILI LOREDANA lucia.rizzo RIZZO LUCIA |