| Title | Spatial distribution of the culturable bacterial community associated with the invasive alga Caulerpa cylindracea in the Mediterranean Sea |
| Abstract | Understanding the mechanisms underlying the complex seaweed-bacteria associations in nature may provide information on the fitness of an invasive host. This may require the use of different approaches. In this study, we employed, for the first time, the Biolog system-Ecoplates(TM) to analyze the functional diversity of the culturable fraction of the bacterial assemblages associated with the surface of Caulerpa cylindracea, the invasive seaweed of the Mediterranean Sea. Seaweed samples were collected at five sites across the basin. A high similarity in the bacterial activity, expressed as Average Well Color Development (AWCD), among the study sites was observed. Culturable heterotrophic bacteria at 22 degrees C showed mean values ranging from 1.4 x 10(5) CFU g(-1) at Porto Cesareo (Ionian Sea, Italy) to 5.8 x 10(6) CFU g(-1) at Othonoi, Diapontine Island (Ionian Sea, Greece). The analysis of the DNA sequences on isolated bacteria demonstrated that the genera Shewanella, Marinobacter, Vibrio, Granulosicoccus and the family Rhodobacteraceae are consistently present on C cylindracea, irrespective of its geographical origin. The present study provided new insights into the complex association between bacteria and this algal species, suggesting a specific composition and function of the associated culturable bacteria across the basin. (C) 2017 Elsevier Ltd. All rights reserved. |
| Source | Marine environmental research 125, pp. 90–98 |
| Keywords | BioinvasionsAlien seaweedsMetabolic profilesBiolog assayFunctional diversityCulturable heterotrophic bacteria |
| Journal | Marine environmental research |
| Editor | Applied Science Publishers,, Barking, Regno Unito |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.marenvres.2017.02.001 |
| Authors | Stabili, Loredana; Rizzo, Lucia; Pizzolante, Graziano; Alifano, Pietro; Fraschetti, Simonetta |
| Text | 400029 2017 10.1016/j.marenvres.2017.02.001 ISI Web of Science WOS 000397076700010 Bioinvasions Alien seaweeds Metabolic profiles Biolog assay Functional diversity Culturable heterotrophic bacteria Spatial distribution of the culturable bacterial community associated with the invasive alga Caulerpa cylindracea in the Mediterranean Sea Stabili, Loredana; Rizzo, Lucia; Pizzolante, Graziano; Alifano, Pietro; Fraschetti, Simonetta Univ Salento; CNR; Univ Ca Foscari Venezia; CoNISMa Consorzio Nazl Interuniv Sci Mare Understanding the mechanisms underlying the complex seaweed bacteria associations in nature may provide information on the fitness of an invasive host. This may require the use of different approaches. In this study, we employed, for the first time, the Biolog system Ecoplates TM to analyze the functional diversity of the culturable fraction of the bacterial assemblages associated with the surface of Caulerpa cylindracea, the invasive seaweed of the Mediterranean Sea. Seaweed samples were collected at five sites across the basin. A high similarity in the bacterial activity, expressed as Average Well Color Development AWCD , among the study sites was observed. Culturable heterotrophic bacteria at 22 degrees C showed mean values ranging from 1.4 x 10 5 CFU g 1 at Porto Cesareo Ionian Sea, Italy to 5.8 x 10 6 CFU g 1 at Othonoi, Diapontine Island Ionian Sea, Greece . The analysis of the DNA sequences on isolated bacteria demonstrated that the genera Shewanella, Marinobacter, Vibrio, Granulosicoccus and the family Rhodobacteraceae are consistently present on C cylindracea, irrespective of its geographical origin. The present study provided new insights into the complex association between bacteria and this algal species, suggesting a specific composition and function of the associated culturable bacteria across the basin. C 2017 Elsevier Ltd. All rights reserved. 125 Published version Articolo in rivista Applied Science Publishers, 0141 1136 Marine environmental research Marine environmental research Mar. environ. res. Marine environmental research. Journal of marine environmental research loredana.stabili STABILI LOREDANA lucia.rizzo RIZZO LUCIA |
