| Title | Biological As(III) oxidation in biofilters by using native groundwater microorganisms |
| Abstract | Arsenic (As) contamination in drinking water represents a worldwide threat to human health. During last decades,
the exploitation of microbial As-transformations has been proposed for bioremediation applications.
Among biological methods for As-contaminated water treatment, microbial As(III)-oxidation is one of the
most promising approaches since it can be coupled to commonly used adsorption removal technologies, without
requiring the addition of chemicals and producing toxic by-products. Despite the As(III) oxidation capability has
been described in several bacterial pure or enrichment cultures, very little is known about the real potentialities
of this processwhenmixed microbial communities, naturally occurring in As contaminatedwaters, are used. This
study highlighted the contribution of native groundwater bacteria to As(III)-oxidation in biofilters, under conditions
suitable for a household-scale treatment system. This work elucidated the influence of a variety of experimental
conditions (i.e., various filling materials, flow rates, As(III) inflow concentration, As(III):As(V) ratio, filter
volumes) on the microbially-mediated As(III)-oxidation process in terms of oxidation efficiency and rate. The
highest oxidation efficiencies (up to 90% in 3 h) were found on coarse sand biofilters treating total initial As
concentration of 100 ?g L-1. The detailed microbial characterization of the As(III) oxidizing biofilms revealed
the occurrence of several OTUs affiliated with families known to oxidize As(III) (e.g., Burkholderiaceae,
Comamonadaceae, Rhodobacteraceae, Xanthomonadaceae). Furthermore, As-related functional genes increased
in biofilter systems in line with the observed oxidative performances. |
| Source | Science of the total environment 651, pp. 93–102 |
| Keywords | Arsenic; Arsenite oxidation; As-related functional genes; Biofilter; Groundwater; Microbiome |
| Journal | Science of the total environment |
| Editor | Elsevier, Lausanne ;, Paesi Bassi |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.scitotenv.2018.09.176 |
| Authors | Crognale S.; Casentini B.; Amalfitano S.; Fazi S.; Petruccioli M.; Rossetti S. |
| Text | 398903 2019 10.1016/j.scitotenv.2018.09.176 Scopus 2 s2.0 85053355488 ISI Web of Science WOS WOS 000447915400009 Arsenic; Arsenite oxidation; As related functional genes; Biofilter; Groundwater; Microbiome Biological As III oxidation in biofilters by using native groundwater microorganisms Crognale S.; Casentini B.; Amalfitano S.; Fazi S.; Petruccioli M.; Rossetti S. Water Research Institute, National Research Council of Italy IRSA CNR , Via Salaria, km 29.300, Monterotondo, Rome, 00015, Italy; Department for Innovation in Agroforestry and Biological systems DIBAF , University of Tuscia, Viterbo, , Italy Arsenic As contamination in drinking water represents a worldwide threat to human health. During last decades, the exploitation of microbial As transformations has been proposed for bioremediation applications. Among biological methods for As contaminated water treatment, microbial As III oxidation is one of the most promising approaches since it can be coupled to commonly used adsorption removal technologies, without requiring the addition of chemicals and producing toxic by products. Despite the As III oxidation capability has been described in several bacterial pure or enrichment cultures, very little is known about the real potentialities of this processwhenmixed microbial communities, naturally occurring in As contaminatedwaters, are used. This study highlighted the contribution of native groundwater bacteria to As III oxidation in biofilters, under conditions suitable for a household scale treatment system. This work elucidated the influence of a variety of experimental conditions i.e., various filling materials, flow rates, As III inflow concentration, As III As V ratio, filter volumes on the microbially mediated As III oxidation process in terms of oxidation efficiency and rate. The highest oxidation efficiencies up to 90% in 3 h were found on coarse sand biofilters treating total initial As concentration of 100 g L 1. The detailed microbial characterization of the As III oxidizing biofilms revealed the occurrence of several OTUs affiliated with families known to oxidize As III e.g., Burkholderiaceae, Comamonadaceae, Rhodobacteraceae, Xanthomonadaceae . Furthermore, As related functional genes increased in biofilter systems in line with the observed oxidative performances. 651 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85053355488 origin=inward Biological As III oxidation in biofilters by using native groundwater microorganisms Biological As III oxidation in biofilters by using native groundwater microorganisms Crognale_STOTEN 18.pdf Articolo in rivista Elsevier 0048 9697 Science of the total environment Science of the total environment Sci. total environ. simona.rossetti ROSSETTI SIMONA stefano.fazi FAZI STEFANO stefano.amalfitano AMALFITANO STEFANO barbara.casentini CASENTINI BARBARA simona.crognale CROGNALE SIMONA |
