Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Biofilm diversity, structure and matrix seasonality in a full-scale cooling tower |
| Abstract | Biofilms commonly colonise cooling water systems, causing equipment damage and interference with the operational requirements of the systems. In this study, next-generation sequencing (NGS), catalysed reporter deposition fluorescence in situ hybridisation (CARD-FISH), lectin staining and microscopy were used to evaluate temporal dynamics in the diversity and structure of biofilms collected seasonally over one year from an open full-scale cooling tower. Water samples were analysed to evaluate the contribution of the suspended microorganisms to the biofilm composition and structure. Alphaproteobacteria dominated the biofilm communities along with Beta- and Gammaproteobacteria. The phototrophic components were mainly cyanobacteria, diatoms and green algae. Bacterial biodiversity decreased from winter to autumn, concurrently with an increase in cyanobacterial and microalgal richness. Differences in structure, spatial organisation and glycoconjugates were observed among assemblages during the year. Overall, microbial variation appeared to be mostly affected by irradiance and water temperature rather than the source of the communities. Variations in biofilms over seasons should be evaluated to develop specific control strategies. |
| Source | Biofouling (Print) |
| Keywords | Biofilmscooling systemsheat exchangerbiodiversitynext-generation sequencingconfocal laser scanning microscope |
| Journal | Biofouling (Print) |
| Editor | Harwood Academic Publishers., New York, Svizzera |
| Year | 2019 |
| Type | Articolo in rivista |
| DOI | 10.1080/08927014.2018.1541454 |
| Authors | L. Di Gregorio, R. Congestri, V. Tandoi, T. R. Neu, S. Rossetti & F. Di Pippo |
| Text | 398760 2019 10.1080/08927014.2018.1541454 ISI Web of Science WOS WOS 000461979400002 Scopus 2 s2.0 85060333604 Biofilms cooling systems heat exchanger biodiversity next generation sequencing confocal laser scanning microscope Biofilm diversity, structure and matrix seasonality in a full scale cooling tower L. Di Gregorio, R. Congestri, V. Tandoi, T. R. Neu, S. Rossetti F. Di Pippo IRSA CNR Universita degli Studi di Roma Tor Vergata Biofilms commonly colonise cooling water systems, causing equipment damage and interference with the operational requirements of the systems. In this study, next generation sequencing NGS , catalysed reporter deposition fluorescence in situ hybridisation CARD FISH , lectin staining and microscopy were used to evaluate temporal dynamics in the diversity and structure of biofilms collected seasonally over one year from an open full scale cooling tower. Water samples were analysed to evaluate the contribution of the suspended microorganisms to the biofilm composition and structure. Alphaproteobacteria dominated the biofilm communities along with Beta and Gammaproteobacteria. The phototrophic components were mainly cyanobacteria, diatoms and green algae. Bacterial biodiversity decreased from winter to autumn, concurrently with an increase in cyanobacterial and microalgal richness. Differences in structure, spatial organisation and glycoconjugates were observed among assemblages during the year. Overall, microbial variation appeared to be mostly affected by irradiance and water temperature rather than the source of the communities. Variations in biofilms over seasons should be evaluated to develop specific control strategies. Published version https //www.scopus.com/record/display.uri eid=2 s2.0 85060333604 origin=resultslist sort=plf f src=s st1=Di Pippo st2=Francesca nlo=1 nlr=20 nls=afprfnm t sid=52bc6f949f35709c6c38b67f63c68c88 sot=anl sdt=aut sl=40 s=AU ID%28%22Di Pippo%2c Francesca%22 15831265700%29 relpos=5 citeCnt=1 searchTerm= Biofilm diversity, structure and matrix seasonality in a full scale cooling tower Biofilm diversity, structure and matrix seasonality in a full scale cooling tower.pdf Articolo in rivista Harwood Academic Publishers. 0892 7014 Biofouling Print Biofouling Print Biofouling Print Biofouling. Print simona.rossetti ROSSETTI SIMONA francesca.dipippo DI PIPPO FRANCESCA |