| Title | Water quality and total microbial load: A double-threshold identification procedure intended for space applications |
| Abstract | During longer-lasting future space missions, water renewal by ground-loaded supplies will become increasingly expensive and unmanageable for months. Space exploration by self-sufficient spacecrafts is thus demanding the development of culture-independent microbiological methods for in-flight water monitoring to counteract possible contamination risks. In this study, we aimed at evaluating total microbial load data assessed by selected early-warning techniques with current or promising perspectives for space applications (i.e., HPC, ATP-metry, qPCR, flow cytometry), through the analysis of water sources with constitutively different contamination levels (i.e., chlorinated and unchlorinated tap waters, groundwaters, river waters, wastewaters). Using a data-driven double-threshold identification procedure, we presented new reference values of water quality based on the assessment of the total microbial load. Our approach is suitable to provide an immediate alert of microbial load peaks, thus enhancing the crew responsiveness in case of unexpected events due to water contamination and treatment failure. Finally, the backbone dataset could help in managing water quality and monitoring issues for both space and Earth-based applications. |
| Source | Frontiers in microbiology 9 |
| Keywords | water qualityconfidence valuesmicrobial contaminationaquatic bacteriaHPCATPqPCRflow cytometry |
| Journal | Frontiers in microbiology |
| Editor | Frontiers Research Foundation,, Lausanne, Svizzera |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.3389/fmicb.2018.02903 |
| Authors | Amalfitano S.; Levantesi C.; Garrelly L.; Giacosa D.; Bersani F.; Rossetti S. |
| Text | 398624 2018 10.3389/fmicb.2018.02903 Scopus 2 s2.0 85058186998 ISI Web of Science WOS WOS 000452263200001 water quality confidence values microbial contamination aquatic bacteria HPC ATP qPCR flow cytometry Water quality and total microbial load A double threshold identification procedure intended for space applications Amalfitano S.; Levantesi C.; Garrelly L.; Giacosa D.; Bersani F.; Rossetti S. Water Research Institute, National Research Council of Italy, Monterotondo, Italy; Water Research Institute, National Research Council of Italy, Monterotondo, Italy; GL Biocontrol, Cap Alpha, Clapiers, France; Centro Ricerche SMAT, Societa Metropolitana Acque Torino S.p.A., Turin, Italy During longer lasting future space missions, water renewal by ground loaded supplies will become increasingly expensive and unmanageable for months. Space exploration by self sufficient spacecrafts is thus demanding the development of culture independent microbiological methods for in flight water monitoring to counteract possible contamination risks. In this study, we aimed at evaluating total microbial load data assessed by selected early warning techniques with current or promising perspectives for space applications i.e., HPC, ATP metry, qPCR, flow cytometry , through the analysis of water sources with constitutively different contamination levels i.e., chlorinated and unchlorinated tap waters, groundwaters, river waters, wastewaters . Using a data driven double threshold identification procedure, we presented new reference values of water quality based on the assessment of the total microbial load. Our approach is suitable to provide an immediate alert of microbial load peaks, thus enhancing the crew responsiveness in case of unexpected events due to water contamination and treatment failure. Finally, the backbone dataset could help in managing water quality and monitoring issues for both space and Earth based applications. 9 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85058186998 origin=inward Water quality and total microbial load A double threshold identification procedure intended for space applications Versione pubblicata Amalfitano_2018_FiM_Threshold procedure for space waters.pdf Articolo in rivista Frontiers Research Foundation, 1664 302X Frontiers in microbiology Frontiers in microbiology Front. microbiol. Frontiers in microbiology simona.rossetti ROSSETTI SIMONA caterina.levantesi LEVANTESI CATERINA stefano.amalfitano AMALFITANO STEFANO BIOWYSE Biocontamination Integrated cOntrol of Wet sYstems for Space Exploration |
