Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbial Community Changes in a Chlorinated Solvents Polluted Aquifer Over the Field Scale Treatment With Poly-3-Hydroxybutyrate as Amendment |
| Abstract | This study investigated the organohalide-respiring bacteria (OHRB) and the supporting microbial populations operating in a pilot scale plant employing poly-3-hydroxybutyrate (PHB), a biodegradable polymer produced by bacteria from waste streams, for the in situ bioremediation of groundwater contaminated by chlorinated solvents. The bioremediation was performed in ground treatment units, including PHB reactors as slow release source of electron donors, where groundwater extracted from the wells flows through before the re-infiltration to the low permeability zones of the aquifer. The coupling of the biological treatment with groundwater recirculation allowed to drastically reducing the contamination level and the remediation time by efficiently stimulating the growth of autochthonous OHRB and enhancing the mobilization of the pollutants. Quantitative PCR performed along the external treatment unit showed that the PHB reactor may efficiently act as an external incubator to growing Dehalococcoides mccartyi, known to be capable of fully converting chlorinated ethenes to innocuous end-products. The slow release source of electron donors for the bioremediation process allowed the establishment of a stable population of D. mccartyi, mainly carrying bvcA and vcrA genes which are implicated in the metabolic conversion of vinyl chloride to harmless ethene. Next generation sequencing was performed to analyze the phylogenetic diversity of the groundwater microbiome before and after the bioremediation treatment and allowed the identification of the microorganisms working closely with organohalide-respiring bacteria. |
| Source | Frontiers in microbiology 9 |
| Keywords | reductive dehalogenationD. mccartyiPHBreductive dehalogenase genesgroundwater circulation wellsbioremediation |
| Journal | Frontiers in microbiology |
| Editor | Frontiers Research Foundation,, Lausanne, Svizzera |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.3389/fmicb.2018.01664 |
| Authors | Matturro, Bruna; Pierro, Lucia; Frascadore, Emanuele; Papini, Marco Petrangeli; Rossetti, Simona |
| Text | 398617 2018 10.3389/fmicb.2018.01664 ISI Web of Science WOS 000439569400006 Scopus 2 s2.0 85050335299 reductive dehalogenation D. mccartyi PHB reductive dehalogenase genes groundwater circulation wells bioremediation Microbial Community Changes in a Chlorinated Solvents Polluted Aquifer Over the Field Scale Treatment With Poly 3 Hydroxybutyrate as Amendment Matturro, Bruna; Pierro, Lucia; Frascadore, Emanuele; Papini, Marco Petrangeli; Rossetti, Simona IRSA CNR; Sapienza Univ Rome This study investigated the organohalide respiring bacteria OHRB and the supporting microbial populations operating in a pilot scale plant employing poly 3 hydroxybutyrate PHB , a biodegradable polymer produced by bacteria from waste streams, for the in situ bioremediation of groundwater contaminated by chlorinated solvents. The bioremediation was performed in ground treatment units, including PHB reactors as slow release source of electron donors, where groundwater extracted from the wells flows through before the re infiltration to the low permeability zones of the aquifer. The coupling of the biological treatment with groundwater recirculation allowed to drastically reducing the contamination level and the remediation time by efficiently stimulating the growth of autochthonous OHRB and enhancing the mobilization of the pollutants. Quantitative PCR performed along the external treatment unit showed that the PHB reactor may efficiently act as an external incubator to growing Dehalococcoides mccartyi, known to be capable of fully converting chlorinated ethenes to innocuous end products. The slow release source of electron donors for the bioremediation process allowed the establishment of a stable population of D. mccartyi, mainly carrying bvcA and vcrA genes which are implicated in the metabolic conversion of vinyl chloride to harmless ethene. Next generation sequencing was performed to analyze the phylogenetic diversity of the groundwater microbiome before and after the bioremediation treatment and allowed the identification of the microorganisms working closely with organohalide respiring bacteria. This study investigated the organohalide respiring bacteria OHRB and the supporting microbial populations operating in a pilot scale plant employing poly 3 hydroxybutyrate PHB , a biodegradable polymer produced by bacteria from waste streams, for the in situ bioremediation of groundwater contaminated by chlorinated solvents. The bioremediation was performed in ground treatment units, including PHB reactors as slow release source of electron donors, where groundwater extracted from the wells flows through before the re infiltration to the low permeability zones of the aquifer. The coupling of the biological treatment with groundwater recirculation allowed to drastically reducing the contamination level and the remediation time by efficiently stimulating the growth of autochthonous OHRB and enhancing the mobilization of the pollutants. Quantitative PCR performed along the external treatment unit showed that the PHB reactor may efficiently act as an external incubator to growing Dehalococcoides mccartyi, known to be capable of fully converting chlorinated ethenes to innocuous end products. The slow release source of electron donors for the bioremediation process allowed the establishment of a stable population of D. mccartyi, mainly carrying bvcA and vcrA genes which are implicated in the metabolic conversion of vinyl chloride to harmless ethene. Next generation sequencing was performed to analyze the phylogenetic diversity of the groundwater microbiome before and after the bioremediation treatment and allowed the identification of the microorganisms working closely with organohalide respiring bacteria. 9 Published version https //www.frontiersin.org/articles/10.3389/fmicb.2018.01664/full Matturro et al., 2018 Articolo Matturro et al.,2018.pdf Articolo in rivista Frontiers Research Foundation, 1664 302X Frontiers in microbiology Frontiers in microbiology Front. microbiol. Frontiers in microbiology simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |