| Title | Long-term anaerobic digestion of food waste at semi-pilot scale: Relationship between microbial community structure and process performances |
| Abstract | Stability and performance of long term semi-continuous Anaerobic Digestion of food waste at semi-pilot scale is here evaluated based on the integration of multiple lines of evidence. In order to elucidate the main microbial components, the core microbiome dynamics were assessed by high-throughput 16 S rRNA gene sequencing over the reactor operation together with the data related to the AD performances. The experimental reactor, after a successful start-up, was operated for more than 200 days at a moderate OLR (Organic Loading Rate) of 1.6 +/- 0.4 g VS L(-1)d(-1). The availability of readily biodegradable substrate, in particular carbohydrates, favored the fermentative functional redundancy of bacteria promoting the rapid accumulation of acetate first, and propionate afterwards, due to limited methanogenesis. The prolonged operation, despite the moderate OLR, nurtured propionate accumulation, because H-2 concentration exceeded the level capable to render the reaction endergonic, hampering the propionate uptake process. The application of a Pulsed Feeding strategy increased the hydrogenotrophic Methanomicrobiales favoring the consumption of propionate most likely through hydrogen utilization. |
| Source | Biomass & bioenergy 118, pp. 55–64 |
| Keywords | Food wasteAnaerobic digestionFeeding modeHigh-throughput 16S rRNA gene sequencingMicrobial communities |
| Journal | Biomass & bioenergy |
| Editor | Pergamon,, New York, Regno Unito |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.biombioe.2018.08.001 |
| Authors | Tonanzi, Barbara; Gallipoli, Agata; Gianico, Andrea; Montecchio, Daniele; Pagliaccia, Pamela; Di Carlo, Marco; Rossetti, Simona; Braguglia, Camilla M. |
| Text | 397516 2018 10.1016/j.biombioe.2018.08.001 ISI Web of Science WOS 000445897300008 Scopus 2 s2.0 85052450034 Food waste Anaerobic digestion Feeding mode High throughput 16S rRNA gene sequencing Microbial communities Long term anaerobic digestion of food waste at semi pilot scale Relationship between microbial community structure and process performances Tonanzi, Barbara; Gallipoli, Agata; Gianico, Andrea; Montecchio, Daniele; Pagliaccia, Pamela; Di Carlo, Marco; Rossetti, Simona; Braguglia, Camilla M. IRSA CNR Stability and performance of long term semi continuous Anaerobic Digestion of food waste at semi pilot scale is here evaluated based on the integration of multiple lines of evidence. In order to elucidate the main microbial components, the core microbiome dynamics were assessed by high throughput 16 S rRNA gene sequencing over the reactor operation together with the data related to the AD performances. The experimental reactor, after a successful start up, was operated for more than 200 days at a moderate OLR Organic Loading Rate of 1.6 / 0.4 g VS L 1 d 1 . The availability of readily biodegradable substrate, in particular carbohydrates, favored the fermentative functional redundancy of bacteria promoting the rapid accumulation of acetate first, and propionate afterwards, due to limited methanogenesis. The prolonged operation, despite the moderate OLR, nurtured propionate accumulation, because H 2 concentration exceeded the level capable to render the reaction endergonic, hampering the propionate uptake process. The application of a Pulsed Feeding strategy increased the hydrogenotrophic Methanomicrobiales favoring the consumption of propionate most likely through hydrogen utilization. 118 Published version https //www.scopus.com/record/display.uri eid=2 s2.0 85052450034 origin=resultslist Long term anaerobic digestion of food waste at semi pilot scale Manuscript 2018 Long term anaerobic digestion Biom and Bioen.pdf Articolo in rivista Pergamon, 0961 9534 Biomass bioenergy Biomass bioenergy Biomass bioenergy Biomass bioenergy. Biomass and bioenergy PAGLIACCIA PAMELA TONANZI BARBARA DI CARLO MARCO simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA andrea.gianico GIANICO ANDREA daniele.montecchio MONTECCHIO DANIELE agata.gallipoli GALLIPOLI AGATA |
