Scheda di dettaglio – i prodotti della ricerca
Dato | Valore |
---|---|
Title | Lipids of the ultra-thin square halophilic archaeon Haloquadratum walsbyi |
Abstract | The lipid composition of the extremely halophilic archaeon Haloquadratum walsbyi was investigated by thin-layer chromatography and electrospray ionization-mass spectrometry. The analysis of neutral lipids showed the presence of vitamin MK-8, squalene, carotene, bacterioruberin and several retinal isomers. The major polar lipids were phosphatidylglycerophosphate methyl ester, phosphatidylglycerosulfate, phosphatidylglycerol and sulfated diglycosyl diether lipid. Among cardiolipins, the tetra-phytanyl or dimeric phospholipids, only traces of bisphosphatidylglycerol were detected. When the cells were exposed to hypolonic medium, no changes in the membrane lipid composition occurred. Distinguishing it from other extreme halophiles of the Halobacteriaceae family, the osmotic stress did not induce the neo-synthesis of cardiolipins in H. walsbyi. The difference may depend oil the three-laminar structure of the cell wall, which differs significantly from that of other Haloarchaea. |
Source | Archaea 2 (3), pp. 177–183 |
Keywords | lipid |
Journal | Archaea |
Editor | Hindawi Publishing Corporation, , |
Year | 2008 |
Type | Articolo in rivista |
DOI | 10.1155/2008/870191 |
Authors | Lobasso, Simona; Lopalco, Patrizia; Mascolo, Giuseppe; Corcelli, Angela |
Text | 397359 2008 10.1155/2008/870191 ISI Web of Science WOS BCI200900070159 lipid Lipids of the ultra thin square halophilic archaeon Haloquadratum walsbyi Lobasso, Simona; Lopalco, Patrizia; Mascolo, Giuseppe; Corcelli, Angela Univ Bari, Dept Med Biochem Biol and Phys, Bari, Italy The lipid composition of the extremely halophilic archaeon Haloquadratum walsbyi was investigated by thin layer chromatography and electrospray ionization mass spectrometry. The analysis of neutral lipids showed the presence of vitamin MK 8, squalene, carotene, bacterioruberin and several retinal isomers. The major polar lipids were phosphatidylglycerophosphate methyl ester, phosphatidylglycerosulfate, phosphatidylglycerol and sulfated diglycosyl diether lipid. Among cardiolipins, the tetra phytanyl or dimeric phospholipids, only traces of bisphosphatidylglycerol were detected. When the cells were exposed to hypolonic medium, no changes in the membrane lipid composition occurred. Distinguishing it from other extreme halophiles of the Halobacteriaceae family, the osmotic stress did not induce the neo synthesis of cardiolipins in H. walsbyi. The difference may depend oil the three laminar structure of the cell wall, which differs significantly from that of other Haloarchaea. 2 Published version Articolo in rivista Hindawi Publishing Corporation 1472 3646 Archaea Archaea giuseppe.mascolo MASCOLO GIUSEPPE |