Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Biodegradation of Diclofenac by the bacterial strain Labrys portucalensis Fl 1 |
| Abstract | Diclofenac (DCF) is a widely used non-steroidal anti-inflammatory pharmaceutical which is detected in the environment at concentrations which can pose a threat to living organisms. In this study, biodegradation of DCF was assessed using the bacterial strain Labrys portucalensis F11. Biotransformation of 70% of DCF (1.7-34 mu M), supplied as the sole carbon source, was achieved in 30 days. Complete degradation was reached via co-metabolism with acetate, over a period of 6 days for 1.7 mu M and 25 days for 34 mu M of DCF. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. DCF degradation by strain F11 proceeds mainly by hydroxylation reactions; the formation of benzoquinone imine species seems to be a central step in the degradation pathway. Moreover, this is the first report that identified conjugated metabolites, resulting from sulfation reactions of DCF by bacteria. Stoichiometric liberation of chlorine and no detection of metabolites at the end of the experiments are strong indications of complete degradation of DCF by strain F11. To the best of our knowledge this is the first report that points to complete degradation of DCF by a single bacterial strain isolated from the environment. |
| Source | Ecotoxicology and environmental safety 152, pp. 104–113 |
| Keywords | DiclofenacLabrys portucalensis F11BiodegradationMetabolitesCo-metabolism |
| Journal | Ecotoxicology and environmental safety |
| Editor | Elsevier [etc.], Amsterdam, Netherlands [etc.], Paesi Bassi |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.ecoenv.2018.01.040 |
| Authors | Moreira, Irina S.; Bessa, Vania S.; Murgolo, Sapia; Piccirillo, Clara; Mascolo, Giuseppe; Castro, Paula M. L. |
| Text | 397351 2018 10.1016/j.ecoenv.2018.01.040 ISI Web of Science WOS 000427807100013 Diclofenac Labrys portucalensis F11 Biodegradation Metabolites Co metabolism Biodegradation of Diclofenac by the bacterial strain Labrys portucalensis Fl 1 Moreira, Irina S.; Bessa, Vania S.; Murgolo, Sapia; Piccirillo, Clara; Mascolo, Giuseppe; Castro, Paula M. L. Univ Catolica Portuguesa Porto; CNR; CNR NANOTEC Diclofenac DCF is a widely used non steroidal anti inflammatory pharmaceutical which is detected in the environment at concentrations which can pose a threat to living organisms. In this study, biodegradation of DCF was assessed using the bacterial strain Labrys portucalensis F11. Biotransformation of 70% of DCF 1.7 34 mu M , supplied as the sole carbon source, was achieved in 30 days. Complete degradation was reached via co metabolism with acetate, over a period of 6 days for 1.7 mu M and 25 days for 34 mu M of DCF. The detection and identification of biodegradation intermediates was performed by UPLC QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. DCF degradation by strain F11 proceeds mainly by hydroxylation reactions; the formation of benzoquinone imine species seems to be a central step in the degradation pathway. Moreover, this is the first report that identified conjugated metabolites, resulting from sulfation reactions of DCF by bacteria. Stoichiometric liberation of chlorine and no detection of metabolites at the end of the experiments are strong indications of complete degradation of DCF by strain F11. To the best of our knowledge this is the first report that points to complete degradation of DCF by a single bacterial strain isolated from the environment. 152 Published version Articolo in rivista Elsevier etc. 0147 6513 Ecotoxicology and environmental safety Ecotoxicology and environmental safety Ecotoxicol. environ. saf. Ecotoxicology and environmental safety. Environmental research. Section B EES sapia.murgolo MURGOLO SAPIA clara.piccirillo PICCIRILLO CLARA giuseppe.mascolo MASCOLO GIUSEPPE |