| Abstract | The lagoons of the Po River delta are potentially exposed to complex mixtures of contaminants, nevertheless, there is a substantial lack of information about the biological effects of these contaminants in the Po delta lagoons. These environments are highly dynamic and the interactions between chemical and environmental stressors could prevent the proper identification of biological effects and their causes. In this study, we aimed to disentangle such interactions focusing on Manila clams, previously exposed to six lagoons of the Po delta, adopting three complementary tools: a) the detailed description via modelling techniques of lagoon dynamics for salinity and water temperature; b) the response sensitivity of a number of target genes (ahr, cyp4, ?-gst, ?-gst, hsp22, hsp70, hsp90, ikb, dbh, ach, cat, Mn-sod, Cu/Zn-sod, cyp-a, flp, grx, TrxP) investigated in clam digestive glands by Real Time PCR; and c) the relevance of DNA adducts determined in clams as markers of exposure to genotoxic chemicals. The lagoons showed specific dynamics, and two of them (Marinetta and Canarin) could induce osmotic stress. A group of genes (ahr, cyp4, Mn-sod, ?-gst, hsp-22, cyp-a, TrxP) seemed to be associated with overall lagoon characteristics as may be described by salinity and its variations. Lagoon modelling and a second group of genes (hsp70, hsp90, cat, ikb, ach, grx, Cu/Zn-sod) also suggested that moderate increases of river discharge may imply worse exposure conditions. Oxidative stress seemed to be associated with such events but it was slightly evident also under normal exposure conditions. DNA adduct formation was mainly associated with overwhelmed antioxidant defences (e.g. low Cu/Zn-sod) or seemingly with their lack of response in due time. In Po delta lagoons, Manila clam can be affected by chemical and environmental factors which can contribute to induce oxidative stress, DNA adduct formation and, ultimately, to affect clam condition and health. |
| Text | 394000 2018 10.1016/j.marenvres.2018.10.010 Scopus 2 s2.0 85055687345 ISI Web of Science WOS WOS 000452943500026 Po river Ruditapes philippinarum Lagoon sediments Lagoon modelling Salinity Gene expressions Oxidative stress DNA adducts Contaminant mixtures Gene expression and genotoxicity in Manila clam Ruditapes philippinarum modulated by sediment contamination and lagoon dynamics in the Po river delta Stefani F.; Casatta N.; Ferrarin C.; Izzotti A.; Maicu F.; Vigano L. CNR National Research Council of Italy, IRSA Water Research Institute, Via del Mulino 19, Brugherio, MB, 20861, , Italy; CNR National Research Council of Italy, ISMAR Marine Sciences Institute in Venice, Castello 2737/f, Venezia, 30122, , , Italy; CNR National Research Council of Italy, ISMAR Marine Sciences Institute in Venice, Castello 2737/f, Venezia, 30122, , , Italy; Department of Health Sciences, University of Genoa, Via Pastore 1, Genoa, 16132, , Italy; IRCCS Policlinico San Martino, Genoa, , , Italy; IRCCS Policlinico San Martino, Genoa, , , Italy The lagoons of the Po River delta are potentially exposed to complex mixtures of contaminants, nevertheless, there is a substantial lack of information about the biological effects of these contaminants in the Po delta lagoons. These environments are highly dynamic and the interactions between chemical and environmental stressors could prevent the proper identification of biological effects and their causes. In this study, we aimed to disentangle such interactions focusing on Manila clams, previously exposed to six lagoons of the Po delta, adopting three complementary tools a the detailed description via modelling techniques of lagoon dynamics for salinity and water temperature; b the response sensitivity of a number of target genes ahr, cyp4, gst, gst, hsp22, hsp70, hsp90, ikb, dbh, ach, cat, Mn sod, Cu/Zn sod, cyp a, flp, grx, TrxP investigated in clam digestive glands by Real Time PCR; and c the relevance of DNA adducts determined in clams as markers of exposure to genotoxic chemicals. The lagoons showed specific dynamics, and two of them Marinetta and Canarin could induce osmotic stress. A group of genes ahr, cyp4, Mn sod, gst, hsp 22, cyp a, TrxP seemed to be associated with overall lagoon characteristics as may be described by salinity and its variations. Lagoon modelling and a second group of genes hsp70, hsp90, cat, ikb, ach, grx, Cu/Zn sod also suggested that moderate increases of river discharge may imply worse exposure conditions. Oxidative stress seemed to be associated with such events but it was slightly evident also under normal exposure conditions. DNA adduct formation was mainly associated with overwhelmed antioxidant defences e.g. low Cu/Zn sod or seemingly with their lack of response in due time. In Po delta lagoons, Manila clam can be affected by chemical and environmental factors which can contribute to induce oxidative stress, DNA adduct formation and, ultimately, to affect clam condition and health. 142 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85055687345 origin=inward Gene expression and genotoxicity in Manila clam R. philippinarum modulated by sediment contamination and lagoon dynamics in the Po river delta file articolo in formato pdf MAR ENV RES 2018 PRINTED VERSION.pdf Articolo in rivista Applied Science Publishers, 0141 1136 Marine environmental research Marine environmental research Mar. environ. res. Marine environmental research. Journal of marine environmental research CASATTA NADIA MAICU FRANCESCO christian.ferrarin FERRARIN CHRISTIAN fabrizio.stefani STEFANI FABRIZIO luigi.vigano VIGANO LUIGI |
