| Abstract | [object Object]Massive antimicrobial use in animal farming is considered as the greatest contributor to the presence of antimicrobial-resistant bacteria (ARB) in food of animal origin. Nevertheless, sewage from treated animals may impact on vegetables grown on fertilised fields, but it is largely unknown whether and to what extent ARB are transferred to vegetables and the human gut. It could be hypothesised that food of animal and vegetal origin have a different role in ARB transfer to the human gut and that different diets could be characterised by different antimicrobial resistance gene (ARG) loads. This study included three groups comprising vegans (n = 26), vegetarians (n = 32) and omnivores (n = 43). Metadata regarding food consumption and anthropometric parameters were collected. Gut microbial communities were investigated by 16S rDNA analysis. Four ARGs (sul2, tetA, blaTEM and strB) were quantified by qPCR. The results showed a lower total load of investigated ARGs in vegan diet (pairwise comparison adjusted results: omnivorous-vegan, P = 0.0119; omnivorous-vegetarian, P = 0.7416; and vegan-vegetarian, P = 0.0119). No significant differences in abundance of each gene separately were found between the three groups. Neither the amount of animal protein nor the occurrence of ARGs was significant in explaining differences in the gut microbial community of individuals, and a large proportion of the differences between community composition (PERMANOVA, 46.87%) was not explained by the analysed variables. The results support the role of omnivorous and vegetarian diets in accumulating ARGs, suggesting a possible role for animal-derived food consumption. |
| Text | 393669 2018 10.1016/j.ijantimicag.2018.07.023 Scopus 2 s2.0 85054158140 microbiome diet Assessing antimicrobial resistance gene load in vegan, vegetarian and omnivore human gut microbiota Losasso C.; Di Cesare A.; Mastrorilli E.; Patuzzi I.; Cibin V.; Eckert E.M.; Fontaneto D.; Vanzo A.; Ricci A.; Corno G. Department of Food Safety, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell Universita 10, Legnaro, , Italy; DISTAV, Universita degli studi di Genova, Corso Europa 26, Genoa, , Italy; DEI, Universita di Padova, Via Gradenigo 6/b, Padua, , Italy; Microbial Ecology Group, CNR ISE, Corso Tonolli 50, Pallanza, Verbania, , Italy; SIAN, ULSS8 Berica, via IV Novembre 46, Vicenza, , Italy object Object Massive antimicrobial use in animal farming is considered as the greatest contributor to the presence of antimicrobial resistant bacteria ARB in food of animal origin. Nevertheless, sewage from treated animals may impact on vegetables grown on fertilised fields, but it is largely unknown whether and to what extent ARB are transferred to vegetables and the human gut. It could be hypothesised that food of animal and vegetal origin have a different role in ARB transfer to the human gut and that different diets could be characterised by different antimicrobial resistance gene ARG loads. This study included three groups comprising vegans n = 26 , vegetarians n = 32 and omnivores n = 43 . Metadata regarding food consumption and anthropometric parameters were collected. Gut microbial communities were investigated by 16S rDNA analysis. Four ARGs sul2, tetA, blaTEM and strB were quantified by qPCR. The results showed a lower total load of investigated ARGs in vegan diet pairwise comparison adjusted results omnivorous vegan, P = 0.0119; omnivorous vegetarian, P = 0.7416; and vegan vegetarian, P = 0.0119 . No significant differences in abundance of each gene separately were found between the three groups. Neither the amount of animal protein nor the occurrence of ARGs was significant in explaining differences in the gut microbial community of individuals, and a large proportion of the differences between community composition PERMANOVA, 46.87% was not explained by the analysed variables. The results support the role of omnivorous and vegetarian diets in accumulating ARGs, suggesting a possible role for animal derived food consumption. 52 Published version https //www.sciencedirect.com/science/article/pii/S092485791830222X via%3Dihub losasso 2018_Losasso et al IntJAntAg.pdf Articolo in rivista Elsevier Science Publishers 0924 8579 International journal of antimicrobial agents Print International journal of antimicrobial agents Print Int. j. antimicrob. agents Print International journal of antimicrobial agents. Print gianluca.corno CORNO GIANLUCA diego.fontaneto FONTANETO DIEGO andrea.dicesare DI CESARE ANDREA estermaria.eckert ECKERT ESTER MARIA |
