| Abstract | We present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both picocyanobacteria were tentatively designated Synechococcus lacustris Tous, formerly described as a metagenome assembled genome (MAG) from the same habitat, and Cyanobium usitatum Tous, described here for the first time. Both strains were purified in unicyanobacterial cultures, and their genomes were sequenced. They are broadly distributed in freshwater systems; the first seems to be a specialist on temperate reservoirs (Tous, Amadorio, Dexter, Lake Lanier, Sparkling), and the second appears to also be abundant in cold environments including ice-covered lakes such as Lake Baikal, Lake Erie or the brackish Baltic Sea. Having complete genomes provided access to the flexible genome that does not assemble in MAGs. We found several genomic islands in both genomes, within which there were genes for nitrogen acquisition, transporters for a wide set of compounds and biosynthesis of phycobilisomes in both strains. Some of these regions of low coverage in metagenomes also included antimicrobial compounds, transposases and phage defence systems, including a novel type III CRISPR-Cas phage defence system that was only detected in Synechococcus lacustris Tous. |
| Text | 392819 2018 10.1111/1462 2920.14377 Scopus 2 s2.0 85054294092 ISI Web of Science WOS 000447549700022 Picocyanobacteria Ecological and genomic features of two widespread freshwater picocyanobacteria Cabello Yeves P.J.; Picazo A.; Camacho A.; Callieri C.; Rosselli R.; Roda Garcia J.J.; Coutinho F.H.; Rodriguez Valera F. Evolutionary Genomics Group, Departamento de Produccion Vegetal y Microbiologia, Universidad Miguel Hernandez, Alicante, , Spain; Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, Valencia, , , Spain; Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, Valencia, , , Spain; Institute of Ecosystem Study CNR, Verbania, , Italy We present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both picocyanobacteria were tentatively designated Synechococcus lacustris Tous, formerly described as a metagenome assembled genome MAG from the same habitat, and Cyanobium usitatum Tous, described here for the first time. Both strains were purified in unicyanobacterial cultures, and their genomes were sequenced. They are broadly distributed in freshwater systems; the first seems to be a specialist on temperate reservoirs Tous, Amadorio, Dexter, Lake Lanier, Sparkling , and the second appears to also be abundant in cold environments including ice covered lakes such as Lake Baikal, Lake Erie or the brackish Baltic Sea. Having complete genomes provided access to the flexible genome that does not assemble in MAGs. We found several genomic islands in both genomes, within which there were genes for nitrogen acquisition, transporters for a wide set of compounds and biosynthesis of phycobilisomes in both strains. Some of these regions of low coverage in metagenomes also included antimicrobial compounds, transposases and phage defence systems, including a novel type III CRISPR Cas phage defence system that was only detected in Synechococcus lacustris Tous. Published version http //www.scopus.com/inward/record.url eid=2 s2.0 85054294092 partnerID=q2rCbXpz Articolo 2018_Environmental_Microbiology_20_10_3757_3771.pdf Articolo in rivista Blackwell Science. 1462 2912 Environmental microbiology Print Environmental microbiology Print Environ. microbiol. Print cristiana.callieri CALLIERI CRISTIANA |
